PHYTOCHEMICAL PROFILING, IN VITRO AND IN VIVO SINGLE AND SYNERGISTIC CYTOTOXIC, ANTI-HEMOLYTIC, ANTIOXIDANT AND HEPATOPROTECTIVE ACTIVITIES OF NIGELLA SATIVA, OLEAEUROPEA, AND ALLIUM SATIVUM

Abstract

Background: The liver is essential for preserving metabolic homeostasis and protecting cells from oxidative stress-related harm. Natural treatments made from medicinal plants have attracted a lot of attention recently because of their possible hepatoprotective, anti-hemolytic, cytotoxic and antioxidant qualities. This study investigated at the individual as well as synergetic impacts of Nigella sativa, Oleaeuropea, and Allium sativum to unravel the intricate biological effects in both in vivo&in vitro settings.

Method: The research involved comprehensive phytochemical profiling of plants to identify bioactive chemicals, evaluating cytotoxic effects using in vitro techniques, assessing anti-hemolytic properties, and examining antioxidant properties of the extracts. To shed light on the oxidative stress and hepatoprotective properties of these plants randomized controlled trial was carried out on 42 albino mice. The mice were randomly divided into7 groups with 6 each. Hepatoxicity was induced by CCL4. The first group was given CMC; second group was treated withsilymarin (200mg/kg). The 3^rd, 4^th, and 5^th groups were treated with plant extract nigella sativa, Allium sativum and Oleaeuropea respectively (200mg/kg). The last group was treated with combined extract of these plants (100mg/kg).

Results: The findings of this study demonstrated that there was significant anti-oxidant and hepatoprotective activity. Significant reduction was noted in ALT, AST and ALP. Furthermore, these plants extract also produced serum protein such as albumin & glutathione peroxidase level.

Conclusion: The findings of this study made a substantial contribution to the complex interactions between the biological activities displayed by these plants and their phytochemical composition.