EXPLORING HPLC ANALYSIS, ANTIOXIDANT POWER, HEMOLYSIS INHIBITION, AND ANTI-MUTAGENIC POTENTIAL IN VARIOUS EXTRACT FRACTIONS OF SESSUVIUM SESSUVOIDES
Main Article Content
Keywords
Sessuvium sessuvoides, Phytochemicals, HPLC, antioxidant, hemolytic, anti-mutagenic
Abstract
Medicinal plants, rich in bioactive compounds, are extensively studied for their therapeutic potential. Sessuvium sessuvoides, a plant from the Cholistan desert, Pakistan, is used in traditional medicine. The aim of present study was to analyze the phytochemical analysis and its antioxidant, cytotoxic and antimutagenic potential of Sessuvium sessuvoides. The quantification of total phenolic and flavonoid content was carried out using the Folin-Ciocalteu and aluminum chloride colorimetric assay respectively. For the identification of phytocompounds, the HPLC was used. DPPH radical scavenging activity was used to determine the antioxidant potential. Cytotoxicity was assessed by RBCs hemolysis and antimutagenic potential was analyzed by Ames assay. Total phenolic compounds in fractions varied widely, ranging from 134.2±4.1 and 279.4±3.1 mg/g expressed as gallic acid equivalents (GAE). SSCE exhibited the highest total phenolic content. The content of flavonoid expressed as catechin equivalents, varied from 208.684±0.4 to 219.737.9±2.2 mg catechin equivalent/g extract. SSCE exhibited the highest total flavonoid content. The HP-LC analysis confirmed the presence of Salicylic acid, Chlorogenic acid, p-coumaric acid, Gallic acid, Caffeic acid, Ferulic acid, Quercetin, hydroxybenzoic acid and Rutin. It exhibits antioxidant activity, with the methanol fraction showing the highest potential. The plant fractions display cytotoxic effects on human erythrocytes, with the ethanol fraction being the least harmful. Notably, all fractions are non-mutagenic, suggesting protective properties against genetic mutations. In conclusion, the study provides compelling evidence of the phytochemical richness of Sessuvium sessuvoides, particularly in terms of phenolic and flavonoid content. This study not only adds to our understanding that Sessuvium sessuvoides has significant antioxidant, antihemolytic and antimutagenic potential. Future research should focus on the identification and isolation of specific bioactive compounds responsible for these health-promoting properties.
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23. GUPTA, A. K. J. Q. S. O. I. M. P. V. 2003. Quality standards of Indian medicinal plants. Volume 1.
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26. HELMY, S. A., EL-MOFTY, S., EL GAYAR, A. M., EL-SHERBINY, I. M., YOUSRA, M. J. B. & PHARMACOTHERAPY 2022. Novel doxorubicin/folate-targeted trans-ferulic acid-loaded PLGA nanoparticles combination: In-vivo superiority over standard chemotherapeutic regimen for breast cancer treatment. 145, 112376.
27. HUDSON, E. A., DINH, P. A., KOKUBUN, T., SIMMONDS, M. S., GESCHER, A. J. C. E. B. & PREVENTION 2000. Characterization of potentially chemopreventive phenols in extracts of brown rice that inhibit the growth of human breast and colon cancer cells. 9, 1163-1170.
28. IQBAL, M., NISAR, J., ADIL, M., ABBAS, M., RIAZ, M., TAHIR, M. A., YOUNUS, M. & SHAHID, M. J. C. 2017. Mutagenicity and cytotoxicity evaluation of photo-catalytically treated petroleum refinery wastewater using an array of bioassays. 168, 590-598.
29. JIMÉNEZ-BONILLA, P., ZHANG, J., WANG, Y., BLERSCH, D., DE-BASHAN, L.-E., GUO, L. & WANG, Y. J. B. T. 2020. Enhancing the tolerance of Clostridium saccharoperbutylacetonicum to lignocellulosic-biomass-derived inhibitors for efficient biobutanol production by overexpressing efflux pumps genes from Pseudomonas putida. 312, 123532.
30. KAHKESHANI, N., FARZAEI, F., FOTOUHI, M., ALAVI, S. S., BAHRAMSOLTANI, R., NASERI, R., MOMTAZ, S., ABBASABADI, Z., RAHIMI, R. & FARZAEI, M. H. J. I. J. O. B. M. S. 2019. Pharmacological effects of gallic acid in health and diseases: A mechanistic review. 22, 225.
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32. KUMAR, S., NARWAL, S., KUMAR, V. & PRAKASH, O. J. P. R. 2011. α-glucosidase inhibitors from plants: A natural approach to treat diabetes. 5, 19.
33. LIN, D., XIAO, M., ZHAO, J., LI, Z., XING, B., LI, X., KONG, M., LI, L., ZHANG, Q. & LIU, Y. J. M. 2016. An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes. 21, 1374.
34. LODOVICI, M., CALDINI, S., MORBIDELLI, L., AKPAN, V., ZICHE, M. & DOLARA, P. J. T. 2009. Protective effect of 4-coumaric acid from UVB ray damage in the rabbit eye. 255, 1-5.
35. MERROUNI, A. J. J. M. E. S., 14 , 153 I., Elachouri. M.(2023) NEMMAP Database: A platform for exploring the Ethnobotanical, Pharmacological, and Phytochemical profile of North-Eastern Morocco Medicinal plants. 160.
36. MRADU, G., SAUMYAKANTI, S., SOHINI, M., ARUP, M. J. I. J. O. P. & RESEARCH, P. 2012. HPLC profiles of standard phenolic compounds present in medicinal plants. 4, 162-167.
37. NASEEM, Z., ZAHID, M., HANIF, M. A. & SHAHID, M. J. P. J. O. E. S. 2020. Environmentally friendly extraction of bioactive compounds from Mentha arvensis using deep eutectic solvent as green extraction media. 29, 3749-3757.
38. NAVEED, M., HEJAZI, V., ABBAS, M., KAMBOH, A. A., KHAN, G. J., SHUMZAID, M., AHMAD, F., BABAZADEH, D., FANGFANG, X., MODARRESI-GHAZANI, F. J. B. & PHARMACOTHERAPY 2018. Chlorogenic acid (CGA): A pharmacological review and call for further research. 97, 67-74.
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Dec 11, 2023
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Abdul Wadood Chishti, Muhammad Akram, Abid Rashid, Sabira Sultana, & Sultan Ayaz. (2023). EXPLORING HPLC ANALYSIS, ANTIOXIDANT POWER, HEMOLYSIS INHIBITION, AND ANTI-MUTAGENIC POTENTIAL IN VARIOUS EXTRACT FRACTIONS OF SESSUVIUM SESSUVOIDES. Journal of Population Therapeutics and Clinical Pharmacology, 30(19), 403-421. https://doi.org/10.53555/jptcp.v30i19.3673
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Author Biographies
Abdul Wadood Chishti
Department of Eastern Medicine, Government College University Faisalabad
Muhammad Akram
Department of Eastern Medicine, Government College University Faisalabad
Abid Rashid
Department of Eastern Medicine, Government College University Faisalabad
Sabira Sultana
Department of Eastern Medicine, Government College University Faisalabad
Sultan Ayaz
Department of Eastern Medicine, Government College University Faisalabad
How to Cite
Abdul Wadood Chishti, Muhammad Akram, Abid Rashid, Sabira Sultana, & Sultan Ayaz. (2023). EXPLORING HPLC ANALYSIS, ANTIOXIDANT POWER, HEMOLYSIS INHIBITION, AND ANTI-MUTAGENIC POTENTIAL IN VARIOUS EXTRACT FRACTIONS OF SESSUVIUM SESSUVOIDES. Journal of Population Therapeutics and Clinical Pharmacology, 30(19), 403-421. https://doi.org/10.53555/jptcp.v30i19.3673