DRUGS PROFILING, QUANTIFICATION OF RIF/RESISTANCE OF MYCOBACTERIUM TUBERCULOSIS BY USING AUTOMATED GENEXPERT: A DIAGNOSTIC CHALLENGE
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Keywords
GeneXpert MTB/RIF system, Drugs profiling, Mycobacterium tuberculosis, RIF/ resistance detected, World Health
Abstract
The study was conducted from February 2022 to January 2023 with permission of the National Tuberculosis Control Program and Tertiary care Hospitals, Peshawar. Among a total of 1,390 individuals, 621 (44.6%) were male and 769 (55.5%) were female. Regarding Mycobacterium tuberculosis types, there were 1,153 cases (83%) of pulmonary TB, 237 cases (17%) of extra-pulmonary TB, 571 cases of Mycobacterium tuberculosis detection with low levels, and no detection of RIF/resistance genes. Additionally, there were 623 cases of Mycobacterium tuberculosis detection with medium levels, with 2 cases of RIF/resistance detected, and 196 cases of Mycobacterium tuberculosis detection with high levels, with 38 cases of RIF/resistance detected. First-line treatment was recommended for 457 patients (32.8%), with a mean of 244 (±106). Second-line treatment was recommended for 40 patients (2.8%), with a mean of 21.4 (±9.2). New patients accounted for 893 cases (64.2%), with a mean of 478.6 (±207). In recent times, there have been significant advancements in desktop technology, particularly the GeneXpert MTB/RIF system. This system has proven to be effective in detecting Mycobacterium tuberculosis in specific population groups, enabling prompt identification of drug resistance in infected patients.
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Awais khan
Department of Biotechnology and Genetic Engineering, Hazara University, Mansehra.
Department of innovative Technology of Biomedical Engineering Devices, Ming Chi University of technology, Taiwan.
Tasneem Noor Mohammad
Department of Microbiology, Northern Border University, Arar, Saudi Arabia.
Aamna Shah
Department of Pharmacy, Women Institute of Learning and Rehabilitation Sciences Abbottabad.
Sumbal Nosheen
Department of Pharmacy, The Children’s Hospital and University of Child Health Sciences, Lahore
Muhammad Zahid Ali
Department of Microbiology, University of Haripur, Haripur.
Department of Microbiology, Yahya Welfare Complex Hospital, Haripur