ANTIOXIDANT EFFECT OF DIFFERENT PARTS OF CASSIA AURICULATA IN STREPTOZOTOCIN INDUCED DIABETIC RATS
Main Article Content
Keywords
antioxidants, Cassia auriculta flower buds extract, Cassia auriculta leaf extract, lipid peroxidation
Abstract
Diabetes is a common chronic disease where therapeutics innovation is much needed. The search for novel antidiabetic molecules can be greatly facilitated by high throughput metabolomic characterization of herbal medicines. Cassia auriculata is a shrub used in ayurvedic medicine and native to India. Oral administration of Cassia auriculta flower buds extract (CFBEt), leaf extract (CLEt) and seeds extract (CSEt) of diabetic rats for 45 days resulted in significant reduction in blood glucose and significant increase in plasma insulin levels. In addition, CFBEt, CLEt and CSEt caused significant increase in the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, reduced glutathione, vitamin C and vitamin E in liver and kidney of diabetic rats with significant decrease in thiobarbituric acid reactive substances (TBARS) and hydroperoxides formation in liver and kidney, suggesting its role in protection against lipid peroxidation induced membrane damage. The antidiabetic and antioxidant effects of CFBEt are more potent than those of CLEt, CSEt and glibenclamide.
References
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27. Raza H, Ahmed I, John A, Sharma AK. Modulation of xenobiotic metabolism and oxidative stress in chronic streptozotcoin induced diabetic rats fed with Momordica charantia fruit extract. J Biochem Mol Toxicol 2000; 14: 131-139.
28. Machin LJ, Bendich A. Free radical tissue damage: protective role of antioxidant nutrient FASEB J 1987; 1: 441-445.
29. Yoshito T, Masayuki M, Hiroshi Y, Makoto M. The effect of -todcopherol as an antioxidant on the oxidation of membrane protein thiols induced by free radicals generated in different sites. Arch Biochem Biophys 1991; 285: 344-350.
30. Murugan P, Pari L. Effect of tetrahydrocurcumin on lipid peroxidation and lipids in streptozotocin - nicotinamide induced diabetic rats. Basic & Clinical Pharmacology & Toxicology. 2006; 99:122-127.
31. Frei B. Ascorbic acid protects lipids in human plasma and LDL against oxidative damage. Am J Clin Nutr 1991; 54: 113S.
32. Infers H, Sies H. The protection by ascorbate and glutathione against microsomal lipid peroxidation is dependent of Vitamin E. Eur J Biochem 1988; 174: 353-389.
33. Meister A, Anderson ME. Glutathione. Annu Rev Biochem 1987; 52: 711-760.
34. Murugan P, Pari L. Antioxidant effect of tetrahydrocurcumin in streptozotocin - nicotinamide induced diabetic rats. Life sciences. 2006; 79: 1720-1728.
35. Murugan P, Pari L. Effect of tetrahydrocurcumin on plasma antioxidants in streptozotocin- nicotinamide induced experimental diabetes. Journal of Basic & Clinical Physiology & Pharmacology. 2006; 17: 231-244.
36. Pari L, Murugan P. Tetrahydrocurcumin prevents brain lipid peroxidation in streptozotocin-induced diabetic rats. Journal of Medicinal Food. 2007; 10 (2): 323-329.
2. Zipora Tietel. Metabolomics of Cassia Auriculata Plant Parts (Leaf, Flower, Bud) and Their Antidiabetic Medicinal Potentials. A Journal of Integrative BiologyVol. 25, No. 5
3. Pari L, Murugan P. Influence of Cassia auriculata flowers on Insulin Receptors in Streptozotocin Induced Diabetic Rats: Studies on Insulin Binding to Erythrocytes. African Journal of Biochemistry Research. 2007; 1 (7): 148-155.
4. Murugan P. Effect of cassia auriculata l on plasma antioxidants in streptozotocinnicotinamide induced experimental diabetes. International Journal of Information Research and Review. Vol. 02, Issue, 05, pp.6930-6934, May, 2015.
5. Shrotri DS, Aiman R. The relationship of the post-absorptive state to the hypoglyceamic action. Ind. J. Med. Res.1960. 48: 162 – 168.
6. Murugan P. Taner’s Cassia (Cassia auriculata L) extract prevents hemoglobin glycation tail tendon collagen properties in experimental diabetic rats. Journal of cell and tissue research. 2010; 10 (1): 2109-2114.
7. Latha M, Pari L . Preventive effects of Cassia auriculata L. flowers on brain lipid peroxidation in rats treated with streptozotocin diabetes. Molecular and Cellular Biochemistry.2003, 243, 23–28.
8. Masiello, P., Broca, C., Gross, R., Roye, M., Manteghetti, M., Hillaire-Buys, D., Novelli, M., Ribes, G. Experimental NIDDM: development of a new model in adult rats administered streptozotocin and nicotinamide. Diabetes. 1998, 47, 224-229.
9. John, A., Lott Turner, K. Evaluation of trinder’s glucose oxidase method for measuring glucose in serum and urine. Clinical chemistry. 1975 21/12, 1754-1760.
10. Fraga, C.G., Leibouitz, B.E., Toppel, A.L. Lipid peroxidation measured as TBARS in tissue slices: characterization and comparison with homogenates and microsomes. Free radical biology and medicine.1988, 4, 155–161.
11. Jiang, Z.Y., Hunt, J.V., Wolff, S.D., 1992. Ferrous sulphate oxidation in the presence of xylenol orange for detection of lipid hydroperoxide in low-density lipoprotein. Analytical biochemistry 202, 384–389.
12. Sinha AK., 1972. Colorimetric assay of catalase. Analytical Biochemistry 47, 389–394.
13. Kakkar, P., Das, B., Viswanathan, P.N., 1984. A modified spectroscopic assay of superoxide dismutase. The indian journal of medical research 21, 130–132.
14. Rotruck, J.T., Pope, A.L., Ganther, H.E., 1973. Selenium biochemical role as a component of glutathione peroxidase purification assay. Science 179, 588–590.
15. Habig, W.H., Pabst, M.J., Jakpoby, W.B., 1974. Glutathione transferase, a first enzymatic step in mercapturic acid formation. The journal of biological chemistry 249, 7130–7139.
16. Omaye, S.T., Turbull, T.D., Sauberlich, H.C., 1979. Selected method for the determination of ascorbic acid in animal cells, tissues and fluids. McCormic DB, Wright DL (eds.) methods. Enzymology academic press, New York 62, 3 – 11
17. Baker, H., Frank, O., Angelis, B., Feingold, S., 1951. Plasma tocopherol in man at various times after ingesting free or acetylated tocopherol. Nutritional report investication 21, 531 – 536.
18. Ellman, G.C., 1959. Tissue sulfhydryl groups. Archives of biochemistry and biophysics. 82, 70–77.
19. Duncan, B.D., 1957. Multiple ranges tests for correlated and heteroscedastic means. Biometrics 13, 359-364.
20. Murugan P. Preventive effects of cassia auriculata on brain lipid peroxidation streptozotocin diabetic rats. International Journal of Information Research and Review .Vol. 02, Issue, 05, pp.6924-6929, May, 2015.
21. Kakkar R, Mantha SV, Radhi J, Prasad K, Kalra J. Increased oxidative stress in rat liver and pancreas during progression of streptozotocin induced diabetes. Clin Sci 1998; 94: 623-632.
22. Feillet-Coudray C, Rock E, Coudray C, Grzelkowska K, Azais-Braesco V, Dardevet D, Mazur A. Lipid peroxidation and antioxidant status in experimental diabetes. Clin Chim Acta 1999; 284: 31-43.
23. Oberley, L.W: Free radicals and diabetes. Free. Radic. Biol. Med. 1988, 5: 113-124.
24. Baynes JW, Thrope SR. Role of oxidative stress in diabetic complications. Diabetes 1999; 48: 1-9.
25. Vucic M, Gavella M, Bozikov V, Ashcroft SJH, Rocic B. Superoxide dismutase activity in lymphocytes and polymorphonuclear cells of diabetic patients. Eur J Clin Chem Clin Biochem 1997; 35: 517-521.
26. Kumuhekar HM, Katyane SS. Altered kinetic attributes of Na+- K+ ATPase acivity in kidney, brain and erythrocyte membrane in alloxan diabetic rats. Ind J Exp Biol 1992; 30: 26–32.
27. Raza H, Ahmed I, John A, Sharma AK. Modulation of xenobiotic metabolism and oxidative stress in chronic streptozotcoin induced diabetic rats fed with Momordica charantia fruit extract. J Biochem Mol Toxicol 2000; 14: 131-139.
28. Machin LJ, Bendich A. Free radical tissue damage: protective role of antioxidant nutrient FASEB J 1987; 1: 441-445.
29. Yoshito T, Masayuki M, Hiroshi Y, Makoto M. The effect of -todcopherol as an antioxidant on the oxidation of membrane protein thiols induced by free radicals generated in different sites. Arch Biochem Biophys 1991; 285: 344-350.
30. Murugan P, Pari L. Effect of tetrahydrocurcumin on lipid peroxidation and lipids in streptozotocin - nicotinamide induced diabetic rats. Basic & Clinical Pharmacology & Toxicology. 2006; 99:122-127.
31. Frei B. Ascorbic acid protects lipids in human plasma and LDL against oxidative damage. Am J Clin Nutr 1991; 54: 113S.
32. Infers H, Sies H. The protection by ascorbate and glutathione against microsomal lipid peroxidation is dependent of Vitamin E. Eur J Biochem 1988; 174: 353-389.
33. Meister A, Anderson ME. Glutathione. Annu Rev Biochem 1987; 52: 711-760.
34. Murugan P, Pari L. Antioxidant effect of tetrahydrocurcumin in streptozotocin - nicotinamide induced diabetic rats. Life sciences. 2006; 79: 1720-1728.
35. Murugan P, Pari L. Effect of tetrahydrocurcumin on plasma antioxidants in streptozotocin- nicotinamide induced experimental diabetes. Journal of Basic & Clinical Physiology & Pharmacology. 2006; 17: 231-244.
36. Pari L, Murugan P. Tetrahydrocurcumin prevents brain lipid peroxidation in streptozotocin-induced diabetic rats. Journal of Medicinal Food. 2007; 10 (2): 323-329.
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Nov 20, 2020
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Murugan P, & Jawahar S. (2020). ANTIOXIDANT EFFECT OF DIFFERENT PARTS OF CASSIA AURICULATA IN STREPTOZOTOCIN INDUCED DIABETIC RATS. Journal of Population Therapeutics and Clinical Pharmacology, 27(4), 55-64. https://doi.org/10.53555/jptcp.v27i4.2425
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Author Biographies
Murugan P
Principal (i/c), Assistant professor in Biochemistry, Faculty of Science, Bharathidhasn University Model College, Vedharanyam-614810, Tamil Nadu, India.
Jawahar S
Assistant professor of Agronomy, Faculty of Agriculture, Annamalai University, Annamalai nagar-608002, Tamil Nadu, India
How to Cite
Murugan P, & Jawahar S. (2020). ANTIOXIDANT EFFECT OF DIFFERENT PARTS OF CASSIA AURICULATA IN STREPTOZOTOCIN INDUCED DIABETIC RATS. Journal of Population Therapeutics and Clinical Pharmacology, 27(4), 55-64. https://doi.org/10.53555/jptcp.v27i4.2425