Evaluation Of Antimicrobial And Cytotoxic Effects Of 1,25 Dihydroxycholecalciferol Formulation- An Invitro Study
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Keywords
1,25 dihydroxycholecalciferol, vitamin D, formulation, antioxidant, anti-inflammatory
Abstract
Background: Vitamin D is a fat-soluble pro-hormone that can be obtained through exposure to the sun or derived from ingested foods or supplements. It is available in two forms: vitamin D2, also known as ergocalciferol, and vitamin D3, also known as cholecalciferol. It is important to remember, however, that many individuals may have inadequate exposure to UV light due to lack of outdoor activity and the use of protective sunscreen lotions and clothing
Aim: To prepare a formulation containing 1,25 dihydroxycholecalciferol. To determine the antimicrobial and cytotoxic effects of the formulation containing 1,25 dihydroxycholecalciferol .
Materials And Methods: In the present study, preparation of the formulation was carried out initially and then the antimicrobial and cytotoxic activity of the formulation was assessed. The antimicrobial activity of the mouthwash formulation was carried out using agar well diffusion method. Different concentrations of nanoparticles were tested against Streptococcus mutans, E.faecalis lactobacillus and Candida albicans. The cytoxicity activity of the mouthwash was assessed using brine shrimp lethality assay method.
Results: The cytotoxic activity was found to be better at low concentration.8 nauplii survived at 5 μL, 10 μL, 20 μL after 24 hours.The antimicrobial activity of the mouthwash formulation showed that the mouthwash formulation had the highest zone of inhibhition seen in 100 μL concentration on C.albicans almost similar to the contrl antibiotic in comparison to the oral pathogens.
Discussion:1,25 dihydroxycholecalciferol has many medicinal properties. Our study has evaluated the concentration of the formulation and has found positive results.
Conclusion: The prepared formulation had better antimicrobial activity and exhibhited less cytotxicity at lower concentrations.
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