Assessment Of Effects of Manipulation of Stem Cell Conditioning Media with Ethyl Gallate in Regenerative Strategies
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Keywords
stem cell, conditioning media, ethyl gallate, proliferation
Abstract
Introduction: In multicellular organisms , stem cells are undifferentiated or partially differentiated cells that can differentiate into various types of cells and proliferate indefinitely to produce more of the same stem cell . stem cell growth requires conditional media. The conditional media refers to the collection of proteins that contains a signal peptide and are processed via the endoplasmic reticulum and golgi apparatus through the classical secretion pathway.
Materials And Methods: Culture the fibroblasts stem cells using ethyl gallate stem cell conditioning medium. Incubated the stem cell at regular time intervals. The culture stem cell were assessed using scratch test assay.
Result: There was increased proliferation of stem cell in ethyl gallate stem cell conditioned media.
Conclusion: Ethyl gallate is a useful stem cell conditioning substance to increase stem cell growth.
References
2. Ibrahim MF, Allam FA. Potential stem cell—Conditioned medium and their derived exosomes versus omeprazole in treatment of experimental model of gastric ulcer. Acta Histochemica. 2022 May 1;124(4):151896.
3. McKee C, Chaudhry GR. Advances and challenges in stem cell culture. Colloids and surfaces B: Biointerfaces. 2017 Nov 1;159:62-77.
4. Nishikawa SI, Jakt LM, Era T. Embryonic stem-cell culture as a tool for developmental cell biology. PNature reviews Molecular cell biology. 2007 Jun;8(6):502-7.
5. Moeller HC, Mian MK, Shrivastava S, Chung BG, Khademhosseini A. A microwell array system for stem cell culture. Biomaterials. 2008 Feb 1;29(6):752-63.
6. Chen KG, Mallon BS, McKay RD, Robey PG. Human pluripotent stem cell culture: considerations for maintenance, expansion, and therapeutics. Cell stem cell. 2014 Jan 2;14(1):13-26.
7. Sasayama S, Hara T, Tanaka T, Honda Y, Baba S. Osteogenesis of multipotent progenitor cells using the epigallocatechin gallate-modified gelatin sponge scaffold in the rat congenital cleft-jaw model. International journal of molecular sciences. 2018 Nov 29;19(12):3803.
8. Lee S, Lee J, Byun H, Kim SJ, Joo J, Park HH. Evaluation of the anti-oxidative and ROS scavenging properties of biomaterials coated with epigallocatechin gallate for tissue engineering. Acta Biomaterialia. 2021 Apr 1;124:166-78.
9. Monzen S, Mori T, Takahashi K, Abe Y, Inanami O, Kuwabara M, Kashiwakura I. The effects of (−)-epigallocatechin-3-gallate on the proliferation and differentiation of human megakaryocytic progenitor cells. Journal of radiation research. 2006 Jun 1;47(2):213-20.
10. Nguyen QV, Duwoon K, Wang SL, Eun JB. Effect of Terminalia nigrovenulosa extracts and their isolated compounds on intracellular ROS generation and MMP expression in HT1080 cells. Research on Chemical Intermediates. 2016 Mar;42(3):2055-73.
11. Van Der Sanden B, Dhobb M, Berger F, Wion D. Optimizing stem cell culture. Journal of cellular biochemistry. 2010 Nov 1;111(4):801-7.
12. Hinsch K, Zupanc GK. Isolation, cultivation, and differentiation of neural stem cells from adult fish brain. Journal of neuroscience methods. 2006 Nov 15;158(1):75-88.
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