FORMULATION, DEVELOPMENT AND EVALUATION OF ANTI-INFLAMMATORY AND ANTI-MICROBIAL EFFECTS OF A NOVEL POLYHERBAL MOUTHWASH - AN IN VITRO STUDY
Main Article Content
Keywords
Herbal Mouthwash, turmeric, ginger, Microbes, Curcumin
Abstract
AIM- The aim of the study was to prepare a poly herbal mouthwash and evaluate its antimicrobial and anti inflammatory efficacy against commercially available herbal mouthwash. The objective was to signify whether the novel herbal combination [extracts of Zingiber officinale(Ginger), Curcuma longa(Turmeric) and Syzygium aromaticum(Clove) (5% v/w)] could be a better alternative mouthwash to commercially available herbal mouthwash.
MATERIALS AND METHODS-
An in vitro study was undertaken in which extract of Zingiber officinale(Ginger), Curcuma longa(Turmeric) and Syzygium aromaticum(Clove) (5% v/w) . Seven different concentrations were prepared and tested against S. mutans, E.Faecalis, C.albicans, S.aureus in MHA agar medium. Plates were incubated aerobically at 37°C for 48 h, and the zone of inhibition was measured using a Vernier caliper. Commercially available herbal mouthwash(HIORA) was used as a control group. The data were analysed by descriptive analytic tests.
RESULTS-
Results of the study showed that the efficacy of novel polyherbal mouthwash showed comparatively less significant antimicrobial properties against the microorganisms as compared to commercially available herbal mouthwash. The minimum inhibitory concentration(MIC) was also found to be very high i.e., 100μg/ml.
CONCLUSION- There was no significant antimicrobial and anti-inflammatory effect of the polyherbal mouthwash as compared to commercially available herbal mouthwash (HIORA). Since this combination is readily available it can be a cost effective alternative to commercially available herbal mouthwash.
References
2. Tarnow D. Clinical Periodontology and Implant Dentistry, 5th edition. Implant Dentistry 2009; 18: 101.
3. Newman MG, Takei H, Klokkevold PR, et al. Newman and Carranza’s Clinical Periodontology E-Book. Elsevier Health Sciences, 2018.
4. Bennett B. Clinical Practice of the Dental Hygienist, 9th ed. Journal of Dental Education 2005; 69: 1393–1393.
5. Ramamurthy J, Lakshmi T. Pharmacological aspects of tea tree oil (TTO) and its role in dentistry-a comprehensive review. J Pharm Biomed Sci, https://www.cabdirect.org/globalhealth/abstract/20123047686 (2011).
6. Kalaga A, Addy M, Hunter B. The Use of 0.2% Chlorhexidine Spray as an Adjunct to Oral Hygiene and Gingival Health in Physically and Mentally Handicapped Adults. Journal of Periodontology 1989; 60: 381–385.
7. Santos A. Evidence-based control of plaque and gingivitis. Journal of Clinical Periodontology 2003; 30: 13–16.
8. Ramamurthy J, Mg V. COMPARISON OF EFFECT OF HIORA MOUTHWASH VERSUS CHLORHEXIDINE MOUTHWASH IN GINGIVITIS PATIENTS: A CLINICAL TRIAL. Asian Journal of Pharmaceutical and Clinical Research 2018; 11: 84.
9. Niveda R, Jaiganesh R. Knowledge and attitude toward mouthwashes and their uses among dental undergraduate and postgraduate students. Drug Invention Today; 12, http://search.ebscohost.com/login.aspx?direct=true&profile=ehost&scope=site&authtype=crawler&jrnl=09757619&AN=137153932&h=b8hjgj1fcHE2oPXhLfoAoXeDDOCJ8YsaPMxStS1lkTjKCs%2FFRMjWXdgSOmVhjJYiV62uI96x0AtPQcv16ScW3w%3D%3D&crl=c (2019).
10. Singh D, Kulkarni P, Jalaluddin M, et al. Comparative evaluation of antiplaque efficacy between essential oils with alcohol-based and chlorhexidine with nonalcohol-based mouthrinses. Journal of International Society of Preventive and Community Dentistry 2017; 7: 36.
11. Simmonds M. Ivan A. Ross, Medicinal plants of the world: Volume 3 Chemical constituents, traditional and modern medicinal uses, Humana Press Inc., New Jersey (2005) ISBN 1-58829-129-4 pp. 623. Phytochemistry 2006; 67: 754–754.
12. Chaudhari AU, Karhadkar VM, Waghmare PF, et al. Comparative Evaluation of Turmeric and Chlorhexidine Gluconate Mouthwash in Prevention of Plaque Formation and Gingivitis: A Clinical and Microbiological Study. The Journal of Contemporary Dental Practice 2011; 12: 221–224.
13. Bliddal H, Rosetzsky A, Schlichting P, et al. A randomized, placebo-controlled, cross-over study of ginger extracts and ibuprofen in osteoarthritis. Osteoarthritis Cartilage 2000; 8: 9–12.
14. Mahady GB, Pendland SL, Yun GS, et al. Ginger (Zingiber officinale Roscoe) and the gingerols inhibit the growth of Cag A+ strains of Helicobacter pylori. Anticancer Res 2003; 23: 3699–3702.
15. Ficker CE, Smith ML, Susiarti S, et al. Inhibition of human pathogenic fungi by members of Zingiberaceae used by the Kenyah (Indonesian Borneo). Journal of Ethnopharmacology 2003; 85: 289–293.
16. Rahmani AH, Shabrmi FMA, Aly SM. Active ingredients of ginger as potential candidates in the prevention and treatment of diseases via modulation of biological activities. Int J Physiol Pathophysiol Pharmacol 2014; 6: 125–136.
17. Giriraju A, Yunus GY. Assessment of antimicrobial potential of 10% ginger extract againstStreptococcus mutans,Candida albicans, andEnterococcus faecalis: Anin vitrostudy. Indian Journal of Dental Research 2013; 24: 397.
18. Shankland WE 2nd. Four common herbs seen in dental practice: properties and potential adverse effects. Cranio 2009; 27: 118–124.
19. Ogodo AC, Ekeleme UG. In-vitro antibacterial activity of garlic cloves and ginger rhizomes on food-borne pathogens. International Journal of Basic and Applied Sciences; 2. Epub ahead of print 2013. DOI: 10.14419/ijbas.v2i4.1347.
20. Antimicrobial Properties Of Extracts Of
21. Moon S-E, Kim H-Y, Cha J-D. Synergistic effect between clove oil and its major compounds and antibiotics against oral bacteria. Archives of Oral Biology 2011; 56: 907–916.
22. Cai L. Compounds fromSyzygium aromaticumPossessing Growth Inhibitory Activity Against Oral Pathogens. Journal of Natural Products 1996; 59: 987–990.
23. Uppuluri S, Ali SL, Nirmala T, et al. A review on Tinospora cordifolia. International Curr Pharmaceutic Rev Res.
24. Agarwal P, Nagesh L, Murlikrishnan. Evaluation of the antimicrobial activity of various concentrations of Tulsi (Ocimum sanctum) extract against Streptococcus mutans: An in vitro study. Indian Journal of Dental Research 2010; 21: 357.
25. Katiraee F. In vitro antifungal activity of essential oils against fluconazole-susceptible and-resistant Candida albicans. DOI: 10.26226/morressier.5ac39995d462b8028d899f6c.
26. Faria TRB, Furletti-Goes VF, Franzini CM, et al. Anti-inflammatory and antimicrobial effects of Zingiber officinale mouthwash on patients with fixed orthodontic appliances. American Journal of Orthodontics and Dentofacial Orthopedics 2021; 159: 21–29.
27. Goyal P. In vitro Evaluation of Antibacterial Activity of Various Crude Leaf Extracts of Indian Sacred Plant, Ocimum sanctum L. British Microbiology Research Journal 2011; 1: 70–78.
28. Rakshanaa TVR, Lakshmi T. Antibacterial efficacy of herbal mouthwash against oral microbes-in vitro assay. Journal of Advanced Pharmacy Education & Research| Jan-Mar; 7, https://www.speronline.com/japer/Articlefile/JAPER_9_2017.pdf (2017).
29. Vinod KS, Sunil KS, Sethi P, et al. A Novel Herbal Formulation versus Chlorhexidine Mouthwash in Efficacy against Oral Microflora. J Int Soc Prev Community Dent 2018; 8: 184–190.
30. Suhag A, Dixit J, Dhan P. Role of curcumin as a subgingival irrigant: a pilot study. Periodontal Practice Today, http://search.ebscohost.com/login.aspx?direct=true&profile=ehost&scope=site&authtype=crawler&jrnl=17423279&AN=37793258&h=fw%2BxPWRq%2FqkYt7nqhD%2FQ3YZOYS0T4rfiifnTjQBBASBlYa4WywPDPYvU2DUW5ovuZqTcHcI%2Bfai4zCgTmZuROA%3D%3D&crl=c (2007).
31. Chatterjee A, Debnath K, Rao NKH. A comparative evaluation of the efficacy of curcumin and chlorhexidine mouthrinses on clinical inflammatory parameters of gingivitis: A double-blinded randomized controlled clinical study. J Indian Soc Periodontol 2017; 21: 132–137.
32. Mahyari S, Mahyari B, Emami SA, et al. Evaluation of the efficacy of a polyherbal mouthwash containing Zingiber officinale, Rosmarinus officinalis and Calendula officinalis extracts in patients with gingivitis: A randomized double-blind placebo-controlled trial. Complement Ther Clin Pract 2016; 22: 93–98.
33. Azizi A, Aghayan S, Zaker S, et al. In Vitro Effect of Zingiber officinale Extract on Growth of Streptococcus mutans and Streptococcus sanguinis. Int J Dent 2015; 2015: 489842.
34. Cousido MC, Tomás Carmona I, García-Caballero L, et al. In vivo substantivity of 0.12% and 0.2% chlorhexidine mouthrinses on salivary bacteria. Clin Oral Investig 2010; 14: 397–402.
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Bhavana Garapati, Saveetha Dental College and Hospitals
Department of Periodontics, Saveetha Dental College and Hospitals, SIMATS
S Rajeshkumar, Saveetha Dental College and Hospitals
Department of Pharmacology, Saveetha Dental College and Hospitals