COMPARISON OF VARIANT GENETICS IN GSTP1 GENE IN LOCAL BREAST CANCER PATIENTS AND HEALTHY INDIVIDUALS
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Keywords
Breast Cancer, GSTP1, Gentic Polymorphism, Alw26I, Ethnic Variation
Abstract
The Glutathione S-transferases P-1 (GSTP1) was Phase II metabolic enzyme caused detoxification of xenobiotics. Polymorphism in this enzyme was seen in different ethnic groups and might be related to difference in metabolism. Some carcinogens and mutagens are considered directly or indirectly involved in developing distinct sort of carcinoma. Single nucleotide polymorphism might lead to varying metabolic activity of resulting enzymes and could be tested for relation with disease occurrence. GSTP1 had two polymorphic sites that had conversions usually in the exon 5, A→G transition by nucleotide 313 near to codon 105 (Ile105↔Val105). A Polymerase Chain Reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis was conducted by using Alw261. All the digests were electrophoresed on a gel with optimized to 3.5% ultrapure agarose. It was found that the mutant genotype G/G was significantly high in breast cancer patients compared to normal individuls
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Naila Rafiq
Department of Biochemistry, Government College Women University Faisalabad-38000, Faisalabad, Pakistan
Sadia Falak
Department of Biochemistry, The University of Faisalabad-38000, Faisalabad,Pakistan.
Irum Javid
Department of Biochemistry, Sardar Bahadur Khan Women, University, Quetta-87300, Pakistan.
Naheed Sajjad
Department of Biotechnology, Sardar Bahadur Khan Women, University, Quetta-87300, Pakistan.
Farah Naz Channa
Department of Biotechnology, Sardar Bahadur Khan Women, University, Quetta-87300, Pakistan.
Farrukh Bashir
Department of Chemistry, Sardar Bahadur Khan Women, University, Quetta-87300, Pakistan.
Tanzila Sahar
Department of Biochemistry, Government College Women University Faisalabad-38000, Faisalabad, Pakistan
Shamaila Rafiq
Departmetnt of BiochemistryUniversity of Agriculture, Faisalabad-38000, Pakistan.
Fiza Munir
Department of Biochemistry, Government College Women University Faisalabad-38000, Faisalabad, Pakistan