ANTIGLYCATION AND ANTI-AGES ACTIVITY OF SULFOSALICYLIC ACID (IN VITRO STUDY)
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Keywords
Hyperglycemia, advanced glycation end products (AGEs), Sulfosalicylic acid, Periodate borohydride, TBA, bovine serum albumin (BSA).
Abstract
Diabetes mellitus is one of the most common diseases that is present nearly all over the world and it is characterized by hyperglycemia. Hyperglycemia results from protein glycation and the continuing increase of AGEs. These AGEs form on biomolecules and possess complex structures that produce protein fluorescence and cross-linking that leads to tissue damage. Salicylic acid is known as a potent inhibitor of glycation and AGEs. A number of its derivatives i.e. salicylate, 5-aminosalicylic acid, acetylsalicylic acid (aspirin), and para-amino salicylic acid are known to have the potential to reduce glycation levels and AGEs. Our study investigated glycation and AGEs inhibitory activity of sulfosalicylic acid, a synthetic compound using glucose-BSA invitro conditions. The study was performed by making sixteen combinations using different concentrations of glucose, inhibitors with BSA and these were incubated at 37oC and 50oC simultaneously for five weeks. Glycation level was assessed by TBA and periodate assays. ELISA measured AGEs. The formation of glycated BSA was quantitated by measuring browning intensity. The results showed that browning was increased from 1st to 5th week of incubation due to an increase in glycation. Our results showed that a 10 mM concentration of sulfosalicylic acid showed a good response to minimize the glycation and AGE production as compared to its lower concentrations. The results of this study demonstrated that sulfosalicylic acid inhibited the glycation of BSA by glucose in a dose-dependent manner. Periodate borohydride assay showed that it is a more suitable glycation method when compared to the TBA method.
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Tanzila Sahar
Department of Biochemistry, Government College Women University Faisalabad-38000, Faisalabad, Pakistan.
Farah Naz Channa
Department of Biotechnology, Sardar Bahadur Khan Women, University, Quetta-87300, Pakistan.
Irum Javid
Department of Biochemistry, Sardar Bahadur Khan Women, University, Quetta-87300, Pakistan.
Shamim Khan
Department of Pharmacy, Sardar Bahadur Khan Women, University, Quetta-87300, Pakistan.
Nageen
Department of Chemistry, Government College Women University Faisalabad-38000, Faisalabad, Pakistan.
Rimsha Saleem
Department of Biochemistry, Government College Women University Faisalabad-38000, Faisalabad, Pakistan.
Naila Rafiq
Department of Biochemistry, Government College Women University Faisalabad-38000, Faisalabad, Pakistan.