COMPLEXIN GENE AND MALE INFERTILITY
Main Article Content
Keywords
Complexin, Expression, Male Infertility, RT-PCR
Abstract
Infertility is the failure to have offspring after one year of regular unprotected intercourse. Acrosomal exocytosis is a prerequisite for successful fertilization and it involves numerous proteins. Complexin genes play an essential role in a controlled secretion of acrosome content. The objective of the present study was to determine the expression of the complexin gene in healthy and infertile male spermatozoa by using Real-Time Polymerase Chain Reaction. The demographic parameters like age, semen volume, sperm count, motility and immotile sperm were analyzed. There was no significant difference in the age and semen volume between normal and infertile groups. A significant difference was observed when the sperm count (p< 0.0000008) and motility (p<0.00000001) were compared between the groups. RT-PCR data analysis showed a fold change difference of 0.0433. From this fold change difference, we determine that our gene of interest is downregulated and the student’s t-test was also applied which also showed that there is downregulation of the complexin gene in infertile men. The difference observed in analyzed data indicates that there is a significant role of the complexin gene in male infertility and their expression was inversely proportional to each other in normal and infertile males and complexin is surely playing a major role in male infertility. However, it is highly recommended that future studies investigate the cause of the down-regulation of the complexin gene as it plays an important role in the exocytosis of sperm nuclei.
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Muhammad Abu-Bakar Sidique
Department of Biosciences, COMSATS University Islamabad, Park Rd, Islamabad Capital Territory 45550, Pakistan
Muhammad Ali khan
Department of Biochemistry, Abbottabad University of Science and Technology Havalian KPK, Pakistan
Dr. Nusrat Shaheen
Department of Biochemistry, Abbottabad University of Science and Technology Havalian KPK, Pakistan
Dr. Humaira Hussain
Department of Biochemistry, Abbottabad University of Science and Technology Havalian KPK, Pakistan
Dr. Iqra khan
Department of Biotechnology, Fatimah Jinnah University Rawalpindi, Pakistan
Dr. Syed Tahir Abbas Shah
Department of Biosciences, COMSATS University Islamabad, Park Rd, Islamabad Capital Territory 45550, Pakistan
Muhammad Irfan
Department of Biosciences, COMSATS University Islamabad, Park Rd, Islamabad Capital Territory 45550, Pakistan
Ayesha Hassan
Department of Biosciences, COMSATS University Islamabad, Park Rd, Islamabad Capital Territory 45550, Pakistan
Tauheed Ahmad
Department of Biochemistry, Biotechnology and Bioinformatics, The Islamia University of Bahawalpur, Pakistan