FLOUXITINE VS. ACETAMINOPHEN IN DEVELOPING ANIMAL AUTISM MODEL: A COMPARATIVE ANALYSIS
Main Article Content
Keywords
autism spectrum disorder, Negative geotaxis,, Surface righting reflex, Nest seeking, swimming performance
Abstract
Abstract
Background: Autism Spectrum Disorder (ASD) includes difficulties in social interaction, communication, and the presence of repetitive behaviors and interests. While the mechanism of ASD remains unclear, a combination of genetic and environmental factors, alongside disruptions in neurotransmission systems, might play significant role in its pathophysiology.
Objective: This study compares the effectiveness of fluoxetine and acetaminophen in developing animal models of autism, providing insights into which drug better elicits ASD-like behaviors.
Methodology: Three groups of Wistar Rats were used in study. Saline group, fluoxetine (FLX) and Acetaminophen(ACET) in which changes in developmental parameters were assessed by comparing body weight, length and eye opening according to standardized protocol. While behavioral assessment tests includes negative geotaxis, surface righting, nest seeking response, forelimb grip strength and swimming performance.
Results: In current study, highly significant changes were observed in motor function of FLX group in negative geotaxis test at PND 6 and 10. However, in surface righting reflex ACET group displayed significant improvement in re-orientation time at PND 9. In battery tests like nest seeking, swimming and forelimb grip strength test FLX group take more time to compete their task as compare to control, while ACET exhibited significant increase in time to perform forelimb grip strength task.
Conclusion: In conclusion, while fluoxetine may exhibit greater efficacy in inducing autism-like behaviors in animal models however it’s chronic and high-dose usage elevated morbidity and mortality rates. Conversely, acetaminophen may offer a safer alternative.
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Sarah Jameel KHAN
Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi,
Shadab Ahmed
Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi,
Sidra Siddiqui
Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, Karachi
Farzana Sadaf
Department of Pharmacology, Faculty of Pharmacy, Hamdard University, Karachi
Hina Ilyas
Department of Pharmacology, Faculty of Pharmacy, Hamdard University, Karachi
Sana Siddiqui
Department of Pharmaceutics, Faculty of Pharmacy, Hamdard University, Karachi
Hira Ahmed
Department of Pharmacognosy, Faculty of Pharmacy, Hamdard University, Karachi
Aatka Ali
Department of Pharmaceutics, Faculty of Pharmacy, Iqra University, Karachi