PPHARMACOGNOSTIC STUDIES OF ELAEAGNUS ANGUSTIFOLIA L. FROM BOI, DISTRICT ABBOTTABAD
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Keywords
Anti-microbial activity, Bioassay, Culture media, Elaeagnus angustifolia L, MDR microbial strains, Nano particles, Pharmacognosy.
Abstract
Elaeagnus and Elaeagnus angustifolia L. are thought to be the most ancient genera in the Elaeagnaceae family. There are perhaps 50–70 species in these families. The microscopic characteristics of the leaves and stems include their size, fracture, odor, size, and flavor. Microscopical analysis reveals vascular bundles, the top and lower leaf surfaces, trichomes, parenchyma, and sclerenchyma cells, as well as the stomatal index. Methanolic, ethanolic, and distilled water extracts of both parts were also studied for their phytochemical properties. The results of phytochemical analysis showed that secondary metabolites, such as sugars, oils, phenols, and alkaloids, among others, exist. The goal of the current study was to evaluate this plant's anti-bacterial, anti-fungal, and antioxidant properties. The anti-bacterial activity was examined using several bacterial strains. Plant shown strong resistance against bacterial strains. The highest zone of inhibition for the bacterial strain Salmonella typhi was seen in the leaf and leaf extract (22mm) and in the stem (21mm). In addition, plants demonstrated potent anti-fungal efficacy against diverse fungi strains. The fungal strain Mycosphaerella citri, which is measured at 15mm in the stem methanolic extract and 17mm in the leaves, has the biggest estimated zone of inhibition. Through DPPH, the antioxidant capacity of plants was also examined. Elaeagnus angustifolia L. demonstrated plants' high antioxidant capability. On plants, cytotoxic and phytotoxic activities were carried out as well. Lemna Minor L. was utilized against the research plant to examine any potential phytotoxic effects. Results indicated that the plant has a high level of phytotoxicity, therefore it will likely be employed as a weedicide going forward. Using brine shrimps (BSLA), cytotoxic activity was also performed on plants; the leaves and stem of the plants displayed cytotoxic effects.
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Aymin Laraib Syed
Department of Botany, Hazara University, Mansehra
Nain Taara Bukhari
Department of Pathology/Microbiology, Bahria University of Medical and Dental College and Health sciences
Tayyaba Bibi
Department of Microbiology, Abbottabad University of Science and Technology
Malik Faizan Shaukat
Department of Horticulture, University of Haripur, Pakistan
Hamid Ali Khan
Directorate of ORIC, Bacha Khan University Charsadda, Khyber Pakhtunkhwa, Pakistan
Nosheen Khalid
Department of food Science and Technology, University of Haripur, Pakistan
Iqbal Nisa
Department of Microbiology, Women University Swabi, Pakistan
Nabila Qayum
Centre for Biotechnology and Microbiology, University of Swat, Pakistan
Atiya Hussain Khowaja
Department of Acute Care, Westways Staffing service, USA
Babar Khan
Centre for Biotechnology and Microbiology, University of Swat, Pakistan
Sayab Khan
Department of Micrbiology, Hazara University, Mansehra, KPK, Pakistan
Syed Shehzad Ali Shah
Department of Botany, Hazara University, Mansehra
Ghulam Mujtaba Shah
Department of Botany, Hazara University, Mansehra