EVALUATION OF ANTIBACTERIAL POTENTIAL OF SECONDARY METABOLITES PRODUCING BACTERIA ISOLATED FROM RHIZOSPHERE OF CALENDULA OFFICINALIS
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Keywords
Antibacterial, Secondary Metabolites, Bacteria, Rhizosphere, Calendula officinalis
Abstract
The main objective of the present study was isolation and identification of bacteria and determination of their secondary metabolites producing ability that had inhibitory activity against pathogenic bacteria. The soil samples were collected from rhizosphere of Calendula officinalis. The samples were serially diluted and streaked on nutrient agar plates. The isolated species were morphologically and biochemically characterized. These isolates were evaluated for production of secondary metabolites. Antimicrobial activity of secondary metabolites was performed against pathogenic bacteria isolated from diabetic foot ulcer patients i.e. Klebseilla pneumonia, Salmonella specie, Pseudomonas specie. and Shigella specie. The bacteria isolated and identified from the rhizosphere were Actinomycetes, Staphylococcus aureus, and Escherichia coli. The secondary metabolites produced by these species were evaluated for their antimicrobial potential against Salmonella spp. Pseudomonas spp., Klebseilla pneumonia and Shigella spp. In crude form. Actinomycetes showed good results against Salmonella as compare to S. aureus and E. coli. These soil isolates had effective antimicrobial potential as crude form of secondary metabolites was evaluated in this study. Thus, it is concluded from this study that these microbes and there products can be used in pure form for the development of novel potent antibiotics for agricultural or pharmaceutical purposes.
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Sidra Farooq
Department of Health and Biological Sciences, Abasyn University, Peshawar, 25000, Khyber Pakhtunkhwa Pakistan
Muhammad Nazir Uddin
Centre for Biotechnology and Microbiology, University of Swat, Pakistan
Sumayya Qayum
Centre for Plants Sciences and Biodiversity, University of Swat, Pakistan
Sudhair Abbas Bangash
Faculty of Life Science, Department of Pharmacy Sarhad University of Science and Information Technology, Peshawar.
Babar Khan
Centre for Biotechnology and Microbiology, University of Swat, Pakistan
Maria Bibi
Department of Health and Biological Sciences, Abasyn University, Peshawar, 25000, Khyber Pakhtunkhwa Pakistan.
Iqbal Nisa
Department of Microbiology, Women University, Swabi, Pakistan
Iqra Fiaz
Department of Botany, Government College Women University, Faisalabad, Pakistan
Zeeshan khan
Department of Microbiology, Khyber Medical University, Peshawar, Pakistan
Fawad Khan
Department of Microbiology, Hazara University Manshera, Pakistan
Amjad Khan
Department of Microbiology, Abbottabad University of Science and Technology Havelian, Khyber Pakhtunkhwa Pakistan.