Evaluation of cytotoxic and pro-apoptotic effects of Annona muricata SeNPs against the lung cancer line (A-549)
Main Article Content
Keywords
cytotoxic, proliferation, differentiation, angiogenesis, death
Abstract
Cancer is the leading cause of death worldwide, with 10 million deaths reported in 2020. Current cancer treatments are inadequate, particularly for advanced-stage patients, with a high fatality rate. Multidrug resistance (MDR) is a significant barrier in cancer treatment. Plant-derived compounds have fewer side effects than chemotherapy and are being investigated for cancer prevention and treatment. The anticancer characteristics of medicinal plants and their bioactive compounds can limit cancer growth by modifying cell proliferation, differentiation, angiogenesis, death, and metastasis. RIn this study, we aimed to biosynthesize SeNPs from A. muricata extract and test their anti-cancer activity against the lung cancer cell line (A549) in vitro. The cell viability assay and dual staining assay results revealed A. muricata mediated SeNPs, decreases the cell growth almost 80% at 40μg/ml concentration and almost 50% of cell death in 20μg/ml concentration. Further studies in animal models and functional studies will be helpful to discovered the anticancer efficacy of AM-SeNPs.
References
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26. K AP, Selvakumar SC, Selvaraj J, Mony U, Veeraraghavan VP, Sekar D, et al. Reviewing the potential application of miR-21 inhibitors in oral cancer therapeutics. Oral Oncol. 2022;125: 105713.
27. Kavarthapu A, Gurumoorthy K. Linking chronic periodontitis and oral cancer: A review. Oral Oncol. 2021;121: 105375.
28. Subramaniam N, Muthukrishnan A. Oral mucositis and microbial colonization in oral cancer patients undergoing radiotherapy and chemotherapy: A prospective analysis in a tertiary care dental hospital. J Investig Clin Dent. 2019;10: e12454.
29. Arjunkumar, R., Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, India, Nanomaterials for the management of periodontal diseases 2018 doi:10.1007/978-3-319-97634-1_11
30. Paulraj J., Nagar P., Antimicrobial efficacy of triphala and propolis-modified glass ionomer cement: An in vitro study, 2020
31. Neppala G., Maiti S., Rajeshkumar S., Ganapathy D.,Antimicrobial efficacy of temporary and permanent denture soft lining material modified by titanium-dioxide nanoparticles-an in-vitro study 2020 International Journal of Dentistry and Oral Science
32. Jacob B., Malli Sureshbabu N., Ranjan M., Ranganath A., Siddique R. The Antimicrobial Effect of Pomegranate Peel Extract versus Chlorhexidine in High Caries Risk Individuals Using Quantitative Real-Time Polymerase Chain Reaction: A Randomized Triple-Blind Controlled Clinical Trial 2021 International Journal of Dentistry 2021: doi10.1155/2021/5563945
2. Cancer. Available online: https://www.who.int/news-room/fact-sheets/detail/cancer (accessed on 31 August 2022).
3. Global Cancer Facts & Figures| American Cancer Society. Available online: https://www.cancer.org/research/cancer-facts-statistics/global.html (accessed on 31 August 2022).
4. Yuan, R.; Hou, Y.; Sun, W.; Yu, J.; Liu, X.; Niu, Y.; Lu, J.J.; Chen, X. Natural Products to Prevent Drug Resistance in Cancer Chemotherapy: A Review. Ann. N. Y. Acad. Sci. 2017, 1401, 19–27.
5. Gullett, N.P.; Ruhul Amin, A.R.M.; Bayraktar, S.; Pezzuto, J.M.; Shin, D.M.; Khuri, F.R.; Aggarwal, B.B.; Surh, Y.J.; Kucuk, O. Cancer Prevention with Natural Compounds. Semin. Oncol. 2010, 37, 258–281. [Google Scholar] [CrossRef]
6. Borchardt, J.K. Natural Product Chemistry for Drug Discovery. Drug News Perspect. 2002, 15, 187–192.
7. Urbi, Z.; Hossain, M.S.; Rahman, K.M.H.; Zayed, T.M. Grape: A Medicinal Fruit Species in the Holy Qur’an and Its Ethnomedicinal Importance. World Appl. Sci. J. 2014, 30, 253–265.
8. Dafni, A.; Böck, B. Medicinal Plants of the Bible-Revisited. J. Ethnobiol. Ethnomed. 2019, 15, 57.
9. Gupta, S.C.; Kim, J.H.; Prasad, S.; Aggarwal, B.B. Regulation of Survival, Proliferation, Invasion, Angiogenesis, and Metastasis of Tumor Cells through Modulation of Inflammatory Pathways by Nutraceuticals. Cancer Metastasis Rev. 2010, 29, 405–434
10. Syed Najmuddin, S.U.F.; Romli, M.F.; Hamid, M.; Alitheen, N.B.; Abd Rahman, N.M.A.N. Anti-Cancer Effect of Annona muricata Linn Leaves Crude Extract (AMCE) on Breast Cancer Cell Line. BMC Complement. Altern. Med. 2016, 16, 311.
11. Ilango, S., Sahoo, D. K., Paital, B., Kathirvel, K., Gabriel, J. I., Subramaniam, K., Jayachandran, P., Dash, R. K., Hati, A. K., Behera, T. R., Mishra, P., & Nirmaladevi, R. (2022). A Review on Annona muricata and Its Anticancer Activity. Cancers, 14(18), 4539. https://doi.org/10.3390/cancers14184539
12. Rady, I., Bloch, M. B., Chamcheu, C. N., Mbeumi, S. B., Anwar, M. R., Mohamed, H., Babatunde, A. S., Kuiate, R., Noubissi, F. K., El Sayed, K. A., Whitfield, G. K., & Chamcheu, J. C. (2018). Anticancer Properties of Graviola (Annona muricata): A Comprehensive Mechanistic Review. Oxidative Medicine and Cellular Longevity, 2018. https://doi.org/10.1155/2018/1826170
13. Kojima, N.; Abe, M.; Suga, Y.; Ohtsuki, K.; Tanaka, T.; Iwasaki, H.; Yamashita, M.; Miyoshi, H. Critical Role of a Methyl Group on the γ-Lactone Ring of Annonaceous Acetogenins in thePotent Inhibition of Mitochondrial Complex I. Bioorg. Med. Chem. Lett. 2013, 23, 1217–1219.
14. Kwon, J., Lee, S. M., Yoon, T. K., & Park, Y. (2019). Selenium nanoparticles: a paradigm shift in the treatment of diseases. Biomaterials research, 23(1), 1-9.
15. Kumar, S., Yadav, R. S., & Yadav, S. K. (2019). Synthesis, characterization, and application of selenium nanoparticles: A review. Journal of nanoscience, 2019.
16. Yajid, A. I., Ab Rahman, H. S., Kai Wong, M. P., & Wan Zain, W. Z. (2018). Potential Benefits of Annona muricata in Combating Cancer: A Review. The Malaysian Journal of Medical Sciences : MJMS, 25(1), 5-15. https://doi.org/10.21315/mjms2018.25.1.2
17. Gavamukulya, Y., Wamunyokoli, F., & El-Shemy, H. A. (2017). Annona muricata: Is the natural therapy to most disease conditions including cancer growing in our backyard? A systematic review of its research history and future prospects. Asian Pacific Journal of Tropical Medicine, 10(9), 835-848. https://doi.org/10.1016/j.apjtm.2017.08.009
18. Zeng, B. B., Wu, Y., Jiang, S., Yu, Q., Yao, Z. J., Liu, Z. H., ... & Wu, Y. L. (2003). Studies on Mimicry of Naturally Occurring Annonaceous Acetogenins: Non‐THF Analogues Leading to Remarkable Selective Cytotoxicity against Human Tumor Cells. Chemistry–A European Journal, 9(1), 282-290.
19. Mahmood, R. I., Kadhim, A. A., Ibraheem, S., Albukhaty, S., S., H., Abbas, R. H., Jabir, M. S., Mohammed, M. K., Nayef, U. M., AlMalki, F. A., & Sulaiman, G. M. (2022). Biosynthesis of copper oxide nanoparticles mediated Annona muricata as cytotoxic and apoptosis inducer factor in breast cancer cell lines. Scientific Reports, 12(1), 1-10. https://doi.org/10.1038/s41598-022-20360-y
20. Iyer, P. R. (2020). Antiproliferative effects on tumor cells of the synthesized gold nanoparticles against Hep2 liver cancer cell line. Egyptian Liver Journal, 10(1), 1-12. https://doi.org/10.1186/s43066-020-0017-4
21. Meenakshisundaram, S., Krishnamoorthy, V., Jagadeesan, Y., Vilwanathan, R., & Balaiah, A. (2020). Annona muricata assisted biogenic synthesis of silver nanoparticles regulates cell cycle arrest in NSCLC cell lines. Bioorganic Chemistry, 95, 103451. https://doi.org/10.1016/j.bioorg.2019.103451
22. Syed Najmuddin, Syed Umar Faruq et al. “Anti-cancer effect of Annona Muricata Linn Leaves Crude Extract (AMCE) on breast cancer cell line.” BMC complementary and alternative
medicine vol. 16,1 311. 24 Aug. 2016, doi:10.1186/s12906-016-1290-y
23. Rosaiah, G., Mangamuri, U. K., Sikharam, A. S., Devaraj, K., Kalagatur, N. K., & Kadirvelu, K. (2022). Biosynthesis of selenium nanoparticles from Annona muricata fruit aqueous extract and investigation of their antioxidant and antimicrobial potentials. Current Trends in Biotechnology and Pharmacy, 16(1), 101-107.
24. Moghadamtousi, S. Z., Kadir, H. A., Paydar, M., Rouhollahi, E., & Karimian, H. (2014). Annona muricata leaves induced apoptosis in A549 cells through mitochondrial-mediated pathway and involvement of NF-κB. BMC complementary and alternative medicine, 14, 299. https://doi.org/10.1186/1472-6882-14-299
25. Sekar D. Implications of long non-coding RNAs (lncRNAs) in the treatment of oral squamous cell carcinoma (OSCC). Oral Oncol. 2022;127: 105812.
26. K AP, Selvakumar SC, Selvaraj J, Mony U, Veeraraghavan VP, Sekar D, et al. Reviewing the potential application of miR-21 inhibitors in oral cancer therapeutics. Oral Oncol. 2022;125: 105713.
27. Kavarthapu A, Gurumoorthy K. Linking chronic periodontitis and oral cancer: A review. Oral Oncol. 2021;121: 105375.
28. Subramaniam N, Muthukrishnan A. Oral mucositis and microbial colonization in oral cancer patients undergoing radiotherapy and chemotherapy: A prospective analysis in a tertiary care dental hospital. J Investig Clin Dent. 2019;10: e12454.
29. Arjunkumar, R., Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Chennai, India, Nanomaterials for the management of periodontal diseases 2018 doi:10.1007/978-3-319-97634-1_11
30. Paulraj J., Nagar P., Antimicrobial efficacy of triphala and propolis-modified glass ionomer cement: An in vitro study, 2020
31. Neppala G., Maiti S., Rajeshkumar S., Ganapathy D.,Antimicrobial efficacy of temporary and permanent denture soft lining material modified by titanium-dioxide nanoparticles-an in-vitro study 2020 International Journal of Dentistry and Oral Science
32. Jacob B., Malli Sureshbabu N., Ranjan M., Ranganath A., Siddique R. The Antimicrobial Effect of Pomegranate Peel Extract versus Chlorhexidine in High Caries Risk Individuals Using Quantitative Real-Time Polymerase Chain Reaction: A Randomized Triple-Blind Controlled Clinical Trial 2021 International Journal of Dentistry 2021: doi10.1155/2021/5563945
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Jun 24, 2023
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Arthi Srinivasan, Rajesh Kumar, Pratibha Ramani, & Karthikeyan Ramalingam. (2023). Evaluation of cytotoxic and pro-apoptotic effects of Annona muricata SeNPs against the lung cancer line (A-549). Journal of Population Therapeutics and Clinical Pharmacology, 30(16), 254-259. https://doi.org/10.47750/jptcp.2023.30.16.033
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How to Cite
Arthi Srinivasan, Rajesh Kumar, Pratibha Ramani, & Karthikeyan Ramalingam. (2023). Evaluation of cytotoxic and pro-apoptotic effects of Annona muricata SeNPs against the lung cancer line (A-549). Journal of Population Therapeutics and Clinical Pharmacology, 30(16), 254-259. https://doi.org/10.47750/jptcp.2023.30.16.033