Biosynthesis of lemon seed conjugated silver nanoparticles lemon seeds and its anti-microbial activity against oral pathogens
Main Article Content
Keywords
Biosynthesis, pathogens, anti-microbial, nanoparticles
Abstract
Introduction: Citrus fruits are native and abundantly grown in South Asia. They are good source of vitamin C and have for ever been in the medical books for their biological/biomedical importance and there is considerable evidence that citrus fruits may help reduce risk and return the growth of several serious diseases this research particularly aims to assess the anti-microbial activity of silver nanoparticles of lemon seeds on oral pathogens since this side is yet to be explored.
Materials and methods: The lemon seed was ground and prepared with silver nanoparticles using silver nitrate and centrifuged using micro pipette, the solution was tested on petri plates where oral pathogens were cultivated the final readings were taken and grab voice plotted using SPSS software.
Results and discussion: The results were overwhelming and almost as good as a standard antibiotic. This could potentially pave way to pharma industry since lemon seeds were never used before.
Conclusion: Silver nanoparticles with lemon seeds shows antimicrobial properties. Further in vitro and in vivo studies are needed.
References
2. Gislene, G.F., N.J. Locatelli, C.F. Paulo and L.S. Giuliana, 2000. Antibacterial activity of plant extracts and phytochemicals on antibiotic resistant bacteria. Braz. J. Microbiol., 31: 247-256.
3. Kawaii, S., T. Yasuhiko, K. Eriko, O. Kazunori, Y. Masamichi, K. Meisaku, ChihiroIto and F. Hiroshi, 2000. Quantitative study of flavonoids in leaves of Citrus plants J. Agric. Food Chem., 48:3865-3871.
4. Sohn, H.Y., K.H. Son, C.S. Know and S.S. Kang, 2004. Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. Phytomedicine, 11: 666-672.
5. Richards RM. Antimicrobial action of silver nitrate. Microbios. 1981 Jan 1;31(124):83-91.
6. Goudarzi M, Mehdipour M, Hajikhani B, Sadeghinejad S, Sadeghi-Nejad B. Antibacterial properties of citrus limon and pineapple extracts on oral pathogenic bacteria (Streptococcus mutans and Streptococcus sanguis). International
Journal of Enteric Pathogens. 2019 Aug 3;7(3):99-103.
7. Segata N, Haake SK, Mannon P, Lemon KP, Waldron L, Gevers D, Huttenhower C, Izard J. Composition of the adult digestive tract bacterial microbiome based on seven mouth surfaces, tonsils, throat and stool samples. Genome biology. 2012 Jun;13:1-8.
8. Lemes RS, Alves CC, Estevam EB, Santiago MB, Martins CH, SANTOS TC, Crotti AE, Miranda ML. Chemical composition and antibacterial activity of essential oils from Citrus aurantifolia leaves and fruit peel against oral pathogenic bacteria. Anais da Academia Brasileira de Ciências. 2018 Apr;90:1285-92.
9. Spacciapoli P, Buxton D, Rothstein D, Friden P. Antimicrobial activity of silver nitrate against periodontal pathogens. Journal of periodontal research. 2001 Apr;36(2):108-13.
10. Thangavelu L, Adil AH, Arshad S, Devaraj E, Mallineni SK, Sajja R, Chakradhar A, Karobari MI. Antimicrobial properties of silver nitrate nanoparticle and its application in endodontics and dentistry: a review of literature. Journal of Nanomaterials. 2021 Nov 9;2021:1-2.
11. Miyake Y, Hiramitsu M. Isolation and extraction of antimicrobial substances against oral bacteria from lemon peel. Journal of food science and technology. 2011 Oct;48:635-9.
12. Daniel AI, Daniyan SY, Busari BM. Preliminary phytochemical and Antimicrobial Activity of Citrus x limon (L) Burm. f.(lemon) leaf extract against some pathogenic microorganisms.
13. Sekar M, Abdullah MZ. Formulation, evaluation and antimicrobial properties of polyherbal toothpaste. Int J Curr Pharm Res. 2016;8(3):105-7.
14. Sathivel et al. 2008; Sekar et al. 2019; Rajeshkumar et al. 2019; Lakshmi et al. 2015; Felicita et al. 2012; Thejeswar and Thenmozhi 2015; Saravanan et al. 2021; Menon and Thenmozhi 2016; Sahu et al. 2014; Wang et al. 2019
15. Thejeswar and Thenmozhi 2015; Saravanan et al. 2021; Menon and Thenmozhi 2016; Sahu et al. 2014; Wang et al. 2019)(Jayaraj et al. 2015; Sekhar et al. 2001; Khalid et al. 2017; Khalid et al. 2016; Putchala et al. 2013
16. Neelakantan et al. 2011; Thejeswar and Thenmozhi 2015; Saravanan et al. 2021; Menon and Thenmozhi 2016; Mohanavel et al. 2020; Priyanka et al. 2017; Babu and Jayaraman 2020; Gopalakannan et al. 2012; Sahu et al. 2014; Wang et al. 2019
17. Gupta et al. 2010; Balagopal and Arjunkumar 2013; Ramesh et al. 2016; Chozhavendhan et al. 2020; Karthiga et al. 2018; Sajan et al. 2011; Asha et al. 2022; Poorni et al. 2019; Venkatesan et al. 2018; Dhinesh et al. 2017
Article Sidebar
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.