Evaluation Of Antioxidant and Anti-Inflammatory Effects Of 1,25 Dihydroxycholecalciferol Formulation- An Invitro Study
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Keywords
1,25 dihydroxycholecalciferol, vitamin D, formulation, antioxidant, anti-inflammatory
Abstract
Background: Vitamin D is a fat-soluble pro-hormone that is mainly obtained through the exposure to sun. Vitamin D is present mainly in two forms: vitamin D2, also known as ergocalciferol, and vitamin D3, also known as cholecalciferol. Vitamin D plays an essential role in maintaining bone metabolism. Due to lifestyle modifications, there is a worldwide reported cases of vitamin D deficiency due to inadequate sun exposure and lack of intake of foods rich in Vitamin D.
Aim: To prepare a formulation containing 1,25 dihydroxycholecalciferol. To determine the antioxidant and anti-inflammatory effects of the formulation containing 1,25 dihydroxycholecalciferol
Materials And Methods: In the present study, preparation of the formulation was carried out initially and then the antioxidant and anti-inflammatory activity of the formulation was assessed. The antioxidant activity of the formulation was carried out using DPPH Assay method and Hydroxyl Radical Scavenging Assay method. The anti-inflamnatory activity of the formulation was assessed using albumin denaturation assay method and egg albumin denaturation assay method.
Results: The antioxidant and anti-inflammatory activity was found to be better at low concentration. At concentrations of 10-30μl, the antioxidant activity of the prepared formulation was similar to that of the standard. The anti-inflammatory activity was found to be highest at 10 μl concentration.
Conclusion: The prepared formulation had better antioxidant and anti-inflammatory activity at lower concentrations.
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