ANTIBIOTIC RESISTANCE PROFILES OF MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA IN CLINICAL ISOLATES: A RETROSPECTIVE STUDY FROM JINNAH POSTGRADUATE MEDICAL CENTRE, PAKISTAN
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Keywords
Multidrug-Resistant Pseudomonas Aeruginosa, Antibiotic Resistance, Clinical Isolates, Retrospective Analysis
Abstract
Objective: Pseudomonas aeruginosa is a major pathogen causing hospital-acquired infections worldwide, with multidrug-resistant (MDR) strains posing a significant public health challenge. This study aimed to investigate the antibiotic resistance profiles of P. aeruginosa isolates from diverse clinical samples to guide effective treatment strategies.
Methodology: This retrospective study was conducted at the Clinical Pathology Laboratory, Jinnah Postgraduate Medical Centre (JPMC), Karachi, Pakistan, from April to December 2021. P. aeruginosa isolates from various clinical specimens were analyzed along with demographic details. Bacterial identification and antimicrobial susceptibility testing were performed using standard microbiological techniques and the Vitek 2 system. Data analysis was carried out using R Studio.
Results: A total of 1066 P. aeruginosa isolates were identified, with the highest frequency (33%) in individuals under 20 years of age. The majority of isolates came from pus (47%) and urine (23%) samples. High resistance rates were observed for amikacin (77%), ceftizoxime (43%), and norfloxacin (40%). In contrast, cefoperazone-sulbactam exhibited the lowest resistance at 15%.
Conclusion: The findings emphasize the need for antibiotic susceptibility testing in managing P. aeruginosa infections. Although amikacin and tobramycin showed high sensitivity, increasing resistance trends for gentamicin and amikacin warrant careful antibiotic selection and monitoring. Treatment should be tailored based on local resistance data, as resistance patterns vary for antibiotics such as piperacillin-tazobactam and imipenem. Regular surveillance of resistance trends is crucial to ensure effective treatment strategies.
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Dr. Amtul Quddos Latif
Associate Professor Pathology, Main Clinical Pathology Laboratory, Jinnah Postgraduate Medical Centre (JPMC), Karachi, Pakistan
Dr. Kauser Saldera
Professor Physiology, Basic Medical Sciences Institute (JPMC), Karachi, Pakistan
Dr. Erum Amir
Assistant Professor, Clinical Pathology, Department of Pathology, Karachi Medical and Dental College (KMDC), Karachi, Pakistan.
Dr. Farhana Zulfiqar
Assistant Professor Pathology, Main Clinical Pathology Laboratory, Jinnah Postgraduate Medical Centre (JPMC), Karachi, Pakistan
Dr. Rabia Arshad
Professor of Pharmacology, Dow International Dental College, Dow University of Health Sciences, Karachi, Pakistan
Dr Shazia Bhutto
Medical Officer, National Institute of Child Health, Karachi, Pakistan