NANOPRESERVED TOMATOES FOR IMPROVED POST HARVEST MANAGEMENT
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Keywords
Chitosan, harpin, chitosan loaded nanoparticles, harpin nanoparticles, post-harvest management, tomato
Abstract
Postharvest management in tomato is crucial for global supply and also to maintain freshness and keeping them in a diseased-free stage for prolonged periods. Nanomaterials have become better choice to preserve the healthiness of the fruit by extending their shelf life and maintaining the pathogen free conditions. In the current study, we have developed harpin (hrp-encoded elicitor of the hypersensitive response) loaded chitosan nanoparticles (CSNPs) for improved post-harvest management in tomato. The newly synthesized harpin loaded CSNPs (CSHNPs) were characterized via UV-Vis Spectroscopy, Transmission Electron Microscopy (TEM), powder X-Ray Diffraction (XRD), Atomic Force Microscopy (AFM) confirming the nanoparticle nature of CSNPs and CSHNPs. Furthermore, topical application of CSNPs and CSHNPs on freshly harvested tomatoes revealed the significant increase in the levels of polyphenol oxidase (PPO), peroxidase (POD) and phenolic compounds. From the current studies, it is evident that CSNPs and CSHNPs can potentially help in controlling post-harvest storage problems in tomato and other fresh vegetable fruits.
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Praveen Mamidala
Department of Biotechnology, Telangana University, Dichpally, Nizambad,
Telangana 503322, India.
Alex Chaturvedi
Department of Plant Sciences, University of Hyderabad, Hyderabad-500046, India
Ebenezer Madam
Department of Biotechnology, Telangana University, Dichpally, Nizambad, Telangana 503322, India.