EFFECT OF WHOLE PLANT EXTRACT OF AGERATUM CONYZOIDES L. AGAINST SOME BACTERIAL STRAINS
Main Article Content
Keywords
Plant Extract, Medicinal Plant, Bacterial Strains, Ageratum conyzoides L
Abstract
The Ageratum conyzoides L. is an annual aromatic herb in different areas of Sialkot in Punjab. Two bacterial species Escherichia coli and Rhodococcus rhodochorus were used to test antibacterial activity of plant extract of billy goat weed. Muller Hinton nutrient agar medium was used by mixing it with bacterial species. The microdilution technique was used for MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentrations) values. Two extracts i.e. methanolic and aqueous extracts were used. The concentration of 100.0 mg/ml formed 14.0 mm2 zone of inhibition against Rhodococcus rhodochorus which was greater zone than other concentrations of aqueous extract. The concentration of 100.0 mg/ml formed 17.0 mm2 zone of inhibition against Rhodococcus rhodochorus which was greater zone than other concentrations of methanolic extract. Escherichia coli formed no any zone of inhibition against both extracts so it was noted as 0.0mm2. Hence, in both of extracts methanolic extract showed greater effectivity against Rhodococcus rhodochorus i.e. 17.0mm2 and 14.0mm2 respectively. Greater inhibition zone mean that plant Ageratum conyzoides L. severely affects the pathogenic bacteria Rhodococcus rhodochorus, so this plant extract may be used as an effective bio-control agent.
References
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19. Panicker, V. P., Haridas, P., Jayavardhanan, K. K., & John, B.(2017). Evaluation of wound healing properties of methanolic extract of Ageratum conyzoides L. Journal of Livestock Science 8, 201-203.
20. Paulsamy, S., &Karthika, K.(2012). Screening of in vitro antioxidant activity of methanolic leaf and root extracts of Hypochaeris radicata L. (Asteraceae). Journal of Applied Pharmaceutical Science, 2(7), 149-154.
21. Quijano, L., Calderon, J. S., Gomez, F., & Rios, T. (1980). Two polymethoxyflavones from Ageratum conyzoides. Phytochemistry, 21(12), 2965-2967.
22. Ravindran J., Samuel T., Alex E., William J. (2012), Adulticidal activity of Ageratum conyzoides Linn. Leaf extracts against three vector mosquito species. Asian Pacific Journal of Tropical Disease, 2(3):177
23. Reddy, S., Kumar, S., & Ganapaty, S. (2012). Evaluation of Ageratum conyzoides Whole Plant for its Anti-Diabetic Activity. Journal of Advanced Scientific Research, 3(02), 67-70.
24. Rizvi, S. M. D., Shakil, S., Zeeshan, M., Khan, M. S., Shaikh, S., Biswas, D., & Kamal, M. A. (2014). An enzoinformatics study targeting polo-like kinases-1 enzyme: Comparative assessment of anticancer potential of compounds isolated from leaves of Ageratum conyzoides. Pharmacognosy Magazine, 10(1), 14.
25. Sharma K.R., (2020), In-vitro antioxidant, antidiabetic and toxic effect of Ageratum conyzoides from Chitwan district Nepal. Journal of Balkumari College. 9(1): 48-54
26. Shin, S. Y., Koh, D., Lim, Y., & Lee, Y. H. (2021). Inhibition of EGR-1-dependent MMP1 transcription by ethanol extract of Ageratum conyzoides in HaCaT keratinocytes. Molecular Biology Reports, 48(1), 1-11.
27. Singh, V., Singh, H., Sharma, G. P., & Raghubanshi, A. S. (2011). Eco-physiological performance of two invasive weed congeners (Ageratum conyzoides L. and Ageratum houstonianum Mill.) in the Indo-Gangetic plains of India. Environmental Monitoring and Assessment, 178, 415-422.
28. Talukdar, T., & Mukherjee, S. K.(2008). Comparative study of cypselas in three common species of Asteraceae. Pleione, 2(1), 147-149.
29. Tedonkeng Pamo E, Tendonkeng F, Kana J R, Khan Payne V, Boukila B, Lemoufouet J, Miegoue E, Nanda AS (2005): A study of the acaricidal properties of an essential oil extracted from the leaves of Ageratum houstonianum. Veterinary Parasitology 128: 319–323.
30. Tennyson, S., Balaraju, K., Park, K., Ravindran, K. J., Eapen, A., & William, S. J. (2012). In vitro antioxidant activity of Ageratum conyzoides L. (Asteraceae). Asian Pacific Journal of Tropical Disease, 2, 712-714.
31. Tennyson, S., Ravindran, J., Eapen, A., & William, J.(2012). Repellent activity of Ageratum conyzoides L. (Asteraceae) leaf extracts against Anophelesstephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Asian Pacific Journal of Tropical Disease, 2(6), 478-480.
32. Tennyson, S., Ravindran, J., Eapen, A., & William, J.(2015). Ovicidal activity of Ageratum conyzoides L. (Asteraceae) leaf extracts against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Asian Pacific Journal of Tropical Disease, 5(3), 199-203.
33. Veiga, A., Maria da Graça, T. T., Rossa, L. S., Mengarda, M., Stofella, N. C., Oliveira, L. J., ... & Murakami, F. S. (2019). Colorimetric microdilution assay: Validation of a standard method for determination of MIC, IC50%, and IC90% of antimicrobial compounds. Journal of Microbiological Methods, 162, 50-61.
34. Verma, A., Rizvi, S. M. D., Shaikh, S., Ansari, M. A., Shakil, S., Ghazal, F., & Rehman, A. (2014). Compounds isolated from Ageratum conyzoides inhibit the activity of matrix metalloproteinases (MMP-2 and MMP-9): An oncoinformatics study. Pharmacognosy Magazine, 10(37), 18.
35. Woldeyes, S., Adane, L., Tariku, Y., Muleta, D., & Begashaw, T. (2012). Evaluation of antibacterial activities of compounds isolated from Sida rhombifolia Linn. Malvaceae). Natural Product Chemical Research, 1(101), 2.
36. Zeeshan, M., Rizvi, S. M. D., Khan, M. S., & Kumar, A. (2012). Isolation, partial purification and evaluation of bioactive compounds from leaves of Ageratum conyzoides L. Excli Journal, 11, 78.
2. Baghaeifar, Z., Hosseininiya, E., Mehrafza, A., Khodaei, L., & Ghadirpour, F. (2022). The Study of Microgametophyte and Megagametophyte Development in Ageratum conyzoides L. Journal of Plant Research, 35(3), 649-663.
3. Bhattacharjee I, Ghosh A, Chandra G (2005): Antimicrobial activity of the essential oil of Cestrum diurnum (L.). African Journal Biotechnology 4: 371–374.
4. Chandra, S., Shahi, A. K., Dutt, P., & Tava, A. (1996). Essential oil composition of Ageratum conyzoides L. from Jammu region of India. Journal of Essential Oil Research, 8(2), 129-134.
5. Chandraker, S. K., Lal, M., Ghosh, M. K., Tiwari, V., Ghorai, T. K., & Shukla, R.(2020). Green synthesis of copper nanoparticles using leaf extract of Ageratum houstonianum Mill. and study of their photocatalytic and antibacterial activities. Nano Express, 1(1), 010033.
6. Egorov, N. S. (1985). Antibiotics: A scientific approach. Mir publishers, Russia, (pp. 440).
7. Ferrara, G., Iapichino, G., Airò, M., Sabatino, L., & Germanà, M. A. (2016, March). Micropropagation of Ageratum conyzoides by nodal segments. In IX International Symposium on In Vitro Culture and Horticultural Breeding 1187 (pp. 29-36).
8. Ferrazzano, G. F., Scioscia, E., Sateriale, D., Pastore, G., Colicchio, R., Pagliuca, C., ...& Pagliarulo, C. (2017). In vitro antibacterial activity of pomegranate juice and peel extracts on cariogenic bacteria. Bio-Medical Research International, 10(1), 1143-1155
9. Hadidy, D., El Sayed, A. M., El Tantawy, M., & El Alfy, T. (2019). Phytochemical analysis and biological activities of essential oils of the leaves and flowers of Ageratum conyzoides L. cultivated in Egypt. Journal of Essential Oil Bearing Plants, 22(5), 1241-1251.
10. Hoi, T. M., Hung, N. H., Huong, L. T., Hau, D. V., Duyen, D. T. H., Setzer, W. N., & Ogunwande, I. A. (2023). Chemical Compositions of Essential Oils and Antimicrobial Activities of Asteraceae: The Leaves of Blumea lacera (Burm. f.) DC., Tridax procumbens (L.), and Ageratum conyzoides., from Vietnam. Journal of Essential Oil Bearing Plants, 26(1), 130-142.
11. Kumar N. (2014), Biological potential of a weed Ageratum conyzoides L.: A review Indo. American Journal Pharmaceutical Research 4(6):2683
12. Kurade, N. P., Jaitak, V., Kaul, V. K., & Sharma, O. P. (2010). Chemical composition and antibacterial activity of essential oils of Lantana camara, Ageratum conyzoides and Eupatorium adenophorum. Pharmaceutical Biology, 48(5), 539-544.
13. Lamsal, A., Devkota, M. P., Shrestha, D. S., Joshi, S., & Shrestha, A. (2019). Seed germination ecology of Ageratum conyzoides L.: A major invasive weed in Nepal. Public Library of Science One, 14(11), e0225430.
14. Lu, X. N., Liu, X. C., Liu, Q. Z., & Liu, Z. L. (2014). Isolation of Insecticidal constituents from the essential oil of Ageratum conyzoides against Liposcelis bostrychophila L. Journal of Chemistry, 2014, 1-6.
15. Ming, L. C. (1999). Ageratum conyzoides: A tropical source of medicinal and agricultural products. Perspectives on new crops and new uses, 1988, 469-473.
16. Njateng, G. S. S., Kuiate, J. R., Gatsing, D., Tamokou, J. D., Mouokeu, R. S., & Kuete, V. (2010). Antidermatophytic activity and dermal toxicity of essential oil from the leaves of Ageratum conyzoides (Asteraceae). Journal of Biological Sciences, 10(5), 448-454.
17. Okunade, A. L. (2002). Ageratum conyzoides L. (Asteraceae). Fitoterapia, 73(1), 1-16.
18. Pamo, E. T., Tendonkeng, F., Kana, J. R., Payne, V. K., Boukila, B., Lemoufouet, J., ... & Nanda, A. S. (2005). A study of the acaricidal properties of an essential oil extracted from the leaves of Ageratum conyzoides. Veterinary Parasitology, 128(3-4), 319-323.
19. Panicker, V. P., Haridas, P., Jayavardhanan, K. K., & John, B.(2017). Evaluation of wound healing properties of methanolic extract of Ageratum conyzoides L. Journal of Livestock Science 8, 201-203.
20. Paulsamy, S., &Karthika, K.(2012). Screening of in vitro antioxidant activity of methanolic leaf and root extracts of Hypochaeris radicata L. (Asteraceae). Journal of Applied Pharmaceutical Science, 2(7), 149-154.
21. Quijano, L., Calderon, J. S., Gomez, F., & Rios, T. (1980). Two polymethoxyflavones from Ageratum conyzoides. Phytochemistry, 21(12), 2965-2967.
22. Ravindran J., Samuel T., Alex E., William J. (2012), Adulticidal activity of Ageratum conyzoides Linn. Leaf extracts against three vector mosquito species. Asian Pacific Journal of Tropical Disease, 2(3):177
23. Reddy, S., Kumar, S., & Ganapaty, S. (2012). Evaluation of Ageratum conyzoides Whole Plant for its Anti-Diabetic Activity. Journal of Advanced Scientific Research, 3(02), 67-70.
24. Rizvi, S. M. D., Shakil, S., Zeeshan, M., Khan, M. S., Shaikh, S., Biswas, D., & Kamal, M. A. (2014). An enzoinformatics study targeting polo-like kinases-1 enzyme: Comparative assessment of anticancer potential of compounds isolated from leaves of Ageratum conyzoides. Pharmacognosy Magazine, 10(1), 14.
25. Sharma K.R., (2020), In-vitro antioxidant, antidiabetic and toxic effect of Ageratum conyzoides from Chitwan district Nepal. Journal of Balkumari College. 9(1): 48-54
26. Shin, S. Y., Koh, D., Lim, Y., & Lee, Y. H. (2021). Inhibition of EGR-1-dependent MMP1 transcription by ethanol extract of Ageratum conyzoides in HaCaT keratinocytes. Molecular Biology Reports, 48(1), 1-11.
27. Singh, V., Singh, H., Sharma, G. P., & Raghubanshi, A. S. (2011). Eco-physiological performance of two invasive weed congeners (Ageratum conyzoides L. and Ageratum houstonianum Mill.) in the Indo-Gangetic plains of India. Environmental Monitoring and Assessment, 178, 415-422.
28. Talukdar, T., & Mukherjee, S. K.(2008). Comparative study of cypselas in three common species of Asteraceae. Pleione, 2(1), 147-149.
29. Tedonkeng Pamo E, Tendonkeng F, Kana J R, Khan Payne V, Boukila B, Lemoufouet J, Miegoue E, Nanda AS (2005): A study of the acaricidal properties of an essential oil extracted from the leaves of Ageratum houstonianum. Veterinary Parasitology 128: 319–323.
30. Tennyson, S., Balaraju, K., Park, K., Ravindran, K. J., Eapen, A., & William, S. J. (2012). In vitro antioxidant activity of Ageratum conyzoides L. (Asteraceae). Asian Pacific Journal of Tropical Disease, 2, 712-714.
31. Tennyson, S., Ravindran, J., Eapen, A., & William, J.(2012). Repellent activity of Ageratum conyzoides L. (Asteraceae) leaf extracts against Anophelesstephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Asian Pacific Journal of Tropical Disease, 2(6), 478-480.
32. Tennyson, S., Ravindran, J., Eapen, A., & William, J.(2015). Ovicidal activity of Ageratum conyzoides L. (Asteraceae) leaf extracts against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Asian Pacific Journal of Tropical Disease, 5(3), 199-203.
33. Veiga, A., Maria da Graça, T. T., Rossa, L. S., Mengarda, M., Stofella, N. C., Oliveira, L. J., ... & Murakami, F. S. (2019). Colorimetric microdilution assay: Validation of a standard method for determination of MIC, IC50%, and IC90% of antimicrobial compounds. Journal of Microbiological Methods, 162, 50-61.
34. Verma, A., Rizvi, S. M. D., Shaikh, S., Ansari, M. A., Shakil, S., Ghazal, F., & Rehman, A. (2014). Compounds isolated from Ageratum conyzoides inhibit the activity of matrix metalloproteinases (MMP-2 and MMP-9): An oncoinformatics study. Pharmacognosy Magazine, 10(37), 18.
35. Woldeyes, S., Adane, L., Tariku, Y., Muleta, D., & Begashaw, T. (2012). Evaluation of antibacterial activities of compounds isolated from Sida rhombifolia Linn. Malvaceae). Natural Product Chemical Research, 1(101), 2.
36. Zeeshan, M., Rizvi, S. M. D., Khan, M. S., & Kumar, A. (2012). Isolation, partial purification and evaluation of bioactive compounds from leaves of Ageratum conyzoides L. Excli Journal, 11, 78.
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Aug 17, 2024
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Javed Kamar, Zainab Fatima, Sajid Hussain, Sohail Kumar, & Adeela Farooq. (2024). EFFECT OF WHOLE PLANT EXTRACT OF AGERATUM CONYZOIDES L. AGAINST SOME BACTERIAL STRAINS. Journal of Population Therapeutics and Clinical Pharmacology, 31(8), 2812-2830. https://doi.org/10.53555/e9aspq52
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Author Biographies
Javed Kamar
Department of Botany, PMAS Arid Agricultural University, Rawalpindi
Zainab Fatima
Department of Botany, Govt. Jinnah Islamia Graduate College, Sialkot
Sajid Hussain
Department of Botany, PMAS Arid Agricultural University, Rawalpindi
Sohail Kumar
Department of Zoology, Govt. Murray Graduate College, Sialkot
Adeela Farooq
Department of Botany, University of Gujrat, Gujrat-Pakistan
How to Cite
Javed Kamar, Zainab Fatima, Sajid Hussain, Sohail Kumar, & Adeela Farooq. (2024). EFFECT OF WHOLE PLANT EXTRACT OF AGERATUM CONYZOIDES L. AGAINST SOME BACTERIAL STRAINS. Journal of Population Therapeutics and Clinical Pharmacology, 31(8), 2812-2830. https://doi.org/10.53555/e9aspq52