IDENTIFICATION OF BEER MICROFLORA THROUGH MICROBIOLOGICAL AND MOLECULAR BIOLOGICAL TECHNIQUES
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Keywords
Microbiological techniques, Molecular biological techniques, spontaneously fermented beer, Non-conventional yeasts, phenotypic traits, genotypic analysis
Abstract
Microbiological and molecular biological techniques play an important role in identification of the beer microflora. The purpose of the project is to identify and assessed the microbial strains of spontaneously ferment beer for their stability to brewing beer. On identification of suitable strain, the similar food-grade will be purchased to produce the approved food premises beer. This project concentrates on two major parts; one is microbiology-based identification of beer microflora which helps in detection of phenotypic traits of the microbial strains. Five industrial media (NA, M-R-S, VRGB, rose Bengal and universal beer agar) were used to evaluate the beer microbial strains. Second part describes the genotypic analysis of microbial strains through PCR, Big dye sequencing and NCBI-BLAST tool. From partial sequence of yeast isolates, Pichia spp. Pichia occidentalis and Candida boidinii is obtained which are non-conventional yeasts. With increasing demand of beer, these non-conventional yeasts are getting attention due to their unique flavour and aroma production. Overall, despite the poor sequencing data which shows ultimately that those species are present in the beer wort sample but due to partial sequence matching, the end results are ambiguous. If the sequences would have more complete, then a better mapped of more specific and reliable strains could be possible. Some limitations of the project are also discussed. This project also provides some suggestion to mitigate those limitations.
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Farwa Saeed
Faculty of Health and Life Sciences, Coventry University
Muhammad Saud Javed
Department of Medicine (MBBS), Yangtze University, China.
Ayesha kousar
Laboratory of Microbiology, department of translational physiology, epidemiology and public health, Merelbeke, Department of Biochemistry and Microbiology, Gent University, Belgium.
Ateeqa Aslam
Laboratory of Virology, department of translational physiology, epidemiology and public health, Merelbeke, Department of Biochemistry and Microbiology, Ghent University Belgium.
Dr Daniel Amund
Faculty of Health and Life Sciences, Coventry University.