A COMPARATIVE STUDY OF IMMUNOHISTOCHEMICAL MARKERS IN THE DETECTION OF EARLY MYOCARDIAL INFARCTION
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Keywords
atherosclerosis, pathology, risk factors, markers
Abstract
immunohistochemical techniques have proven effective in forensic pathology use as they can be applied to tissue samples fixed in formalin and embedded in paraffin. In this work, we selected a panel of antibodies, desmin, troponin T (cTnT), and myoglobin (MB), for the detection of early
myocardial infarction in human autopsy hearts with suspected sudden cardiac death to compare the sensitivity and the reliability of these markers and to study the potential effect of autolysis on their sensitivity. Histologic sections from the myocardium from 20 autopsy cases were studied by immunohistochemistry with the three different antibodies, using a standard avidin–biotin–peroxidise system. Desmin, cTnT, and MB depletion occurred very
early after ischemic injury and preceded the histologic
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Dr Ritu Saxena
Associate professor, department of pathology , IQ city foundation, Durgapur