TO STUDY THE ANTIBACTERIAL ACTIVITY OF RICINUS COMMUNIS EXTRACTS AGAINST COMMON BACTERIAL ISOLATES OBTAINED FROM SURGICAL SITE INFECTION IN A TERTIARY CARE HOSPITAL AT KANPUR, UTTAR PRADESH
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Keywords
SSI, Disc diffusion, Kirby-Bauer, Antibacterial activity, Plant extract
Abstract
INTRODUCTION: There are many natural crude drugs from plants that have the potential to treat many diseases, in healing wounds and disorders and one of them is Ricinus communis.
OBJECTIVE: To study the antibacterial activity of Ricinus communis extracts against common bacterial isolates obtained from surgical site infection at a tertiary care hospital in Kanpur, Uttar Pradesh.
METHODS: This was a cross sectional study carried out in the Departments of Microbiology and Medicine Department of Rama Medical College Hospital and Research Centre, Kanpur. A total of 20 SSI infections, were studied, the pus samples were cultured and studied for identification & Antimicrobial susceptibility was performed according to CLSI guidelines 2023. The antimicrobial activity of Ricinus communis (castor oil) was studied. The Ricinus communis plant leaves were collected from the medical garden of RMCH&RC where the leaves were crushed cleaned with distilled water and different solvents of the extracts were prepared and impregmented with 20ul of the plant extract in prepared whatsmann paper disc.
RESULTS: Out of 45 pus samples studied, 20 were of SSI, 18 were from ear infection and 7 were from other samples in Gram positive organisms were 100% sensitive to Linezolid, and Gram negative organisms were 100% sensitive to Colistin. In the present study the crude extract of Ricinus communis leafs was most effective with methanol solvent in350 mg/mL. The highest zone of inhibition was observed against Staphylococcus aureus (22±0.2 mm) followed by Escherichia coli (19±0.3 mm), Klebsiella pneumoniae (18±0.3 mm) and least for for Pseudomonas aeruginosa (9±0.3 mm). It was noted that gram positive bacteria had significantly shown more the sensitive zones of inhibition as compared to the gram negative bacteria.
CONCLUSION: This study supports the antibacterial properties of Ricinus communis leaf extraction be used to treat various diseases like SSI.
References
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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Priyanka Raikwar
PG Student, Department of Microbiology, Rama Medical College Hospital Research Centre, Kanpur.
R. Sujatha
Professor and Head, Department of Microbiology, Rama Medical College Hospital Research Centre, Kanpur.
Nashra Afaq
Research Associate, Department of Microbiology, Rama Medical College Hospital Research Centre, Kanpur.
Mahesh Gupta
Professor and Head, Department of General Surgery, Rama Medical College Hospital Research Centre, Kanpur.