“A STUDY ON ANTIFUNGAL SUSCEPTIBILITY PATTERN, MOLECULAR PROFILING AND ITS SEQUENCING ANALYSIS OF TRICHOPHYTON RUBRUM ISOLATED FROM A TERTIARY CARE CENTRE”.
Main Article Content
Keywords
Antifungal Susceptibility, Trichophyton rubrum, Molecular Profiling, DNA, PCR, Sequencing
Abstract
Introduction: Trichophyton rubrum is the most common aetiological agent of human dermatophytoses. These infections mainly occur in keratinised layers such as skin, hair and nails because the fungus uses keratin as a nutrient source. T. rubrum being the most common etiological agent isolated from clinically diagnosed lesions of cutaneous dermatophytosis. It has a significantly higher capacity of transmission than other anthropophilic dermatophytes.
Aim and Objective: To study the Antifungal Susceptibility Pattern , Molecular Profiling and its Sequencing Analysis of Trichophyton rubrum isolated from patients.
Material and Methods : This was a prospective study carried out in the Department of Microbiology. A total of 380 clinically suspected cases of superficial fungal infection were collected with proper informed consent. Dermatophytosis was confirmed in cases by direct microscopy and culture methods. Antifungal drug susceptibility profiles, MIC of isolates were 50 90 determined using the broth microdilution method according to the CLSI guidelines 2022. The DNA isolation was done using the Qiagen DNA extraction kit followed by the conventional PCR. The sequencing methods were used for the genetic analysis of virulence gene for the confirmation.
Results : In the present study out of the total of 380 clinically suspected cases of superficial fungal infection 39 isolates of Trichophyton rubrumwere studied. The ratio of Male were more 32 (82.05%) as compared to the Female 7(17.94% ). The maximum number of isolates was found in the Skin 36(92.30%) followed by the Nail 3 (7.69%). The ITZ was most sensitive toward T. rubrum than KTZ, TBF, and FCZ. The molecular characterization for the detection of 18s gene was performed which was confirmed by the Sequencing Analysis.
Conclusion : The fungal genome's genetic diversity has led to an increase in antifungal resistance against T. rubrum during the past few decades. The gold standard method for identifying T. rubrum is molecular identification, which plays a significant role in the development of strategies for the prevention and treatment of fungal infections.
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Deepak Kumar
Assistant Professor and Head1, Department of Microbiology, Uma Nath Singh Autonomous State Medical College, Jaunpur, India
Gautam Kumar Das
Junior Resident-3rd Year2, Department of Biochemistry, Assam Medical College & Hospital, Dibrugarh, Assam, India
Deepika Shukla
Assistant Professor and Head3, Department of Microbiology, Maharana Pratap Dental College and Hospital Kothi, Mandhana, Kanpur, Uttar Pradesh, India.
Nashra Afaq
Research Associate4, Department of Microbiology and Central Research Laboratory, Rama Medical College Hospital and Research Centre, Mandhana, Kanpur, Uttar Pradesh, India
Deepak Shukla
Anesthesiologist & Critical Care Specialist5, Department of Anesthesia, Indian Naval Hospital Kalyani, VIZAG, Andhra Pradesh, India.
Amit Kumar Singh
Assistant Professor, Department of Microbiology6, Prasad Institute of Medical sciences, Lucknow, India.