EFFECTS OF MORINGA LEAVES EXTRACT ON GLUCOSE HOMEOSTASIS, AND INSULIN RESISTANCE IN DIABETES INDUCED RABBITS
Main Article Content
Keywords
Moringa alofera leave extract, Diabetese Mellitus, Insulin resistance
Abstract
Introdcution: Diabetes mellitus (DM) stands out as a significant worldwide public health issue, given its substantial contribution to cardiac-related fatalities, vision impairment, kidney dysfunction, as well as its association with depression and suicide. Moringa alofera leave extract (MOLE) has shown novel biological activity against many diseases including Diabetese Mellitus.
Aim: This pre-clinical study trail on animal model is intended to determine the effect of Moringa alofera on the regulation of glucose homeostasis in diabetes induced rabbits.
Methodology: Twenty-four rabbits of both genders and 1-2 kg of weight were included in the study. Rabbits were divided into 4 groups (i.e., Group-A = non-diabetic control, Group-B = Diabetic control, Group-C = Diabetic Rabbits treated with 200 mg/kg of MOLE, and Group-D = Diabetic Rabbits treated with 400mg/kg of MOLE). Data was analysised using SPSS version 21.
Results: Final fasting blood sugar levels reduced significanty in experimental groups as compared to controls; p <0.05. While, HbA1c levels reduced insignificantly in experimental groups than controls; p > 0,05. Insulin resistance significantly improved in experimental groups than controls, p > 0.05.
Conclusion: As per study conclusion the glucose homeostasis and insulin resistance were observed to be improved in Rabbits treated with Moringa leaves extract.
References
2. Akhtar S, Khan Z, Rafiq M, Khan A. Prevalence of type II diabetes in District Dir Lower in Pakistan. Pakistan journal of medical sciences. 2016 May;32(3):622.
3. Galicia-Garcia U, Benito-Vicente A, Jebari S, Larrea-Sebal A, Siddiqi H. Pathophysiology of type 2 diabetes mellitus. International journal of molecular sciences. 2020 Aug 30;21(17):6275.
4. Tsalamandris S, Antonopoulos AS, Oikonomou E, Papamikroulis GA, Vogiatzi G, Papaioannou S, Deftereos S, Tousoulis D. The role of inflammation in diabetes: current concepts and future perspectives. European cardiology review. 2019 Apr;14(1):50.
5. Rahman MS, Hossain KS, Das S, Kundu S, Adegoke EO, Rahman MA. Role of insulin in health and disease: an update. International journal of molecular sciences. 2021 Jun 15;22(12):6403..
6. Imamura F, Mukamal KJ, Meigs JB, Luchsinger JA, Ix JH, Siscovick DS, Mozaffarian D. Risk factors for type 2 diabetes mellitus preceded by β-cell dysfunction, insulin resistance, or both in older adults: the Cardiovascular Health Study. American journal of epidemiology. 2013 Jun 15;177(12):1418-29.
7. Guo, S., Dai, C., Guo, M., Taylor, B., Harmon, J. S., Sander, M., Robertson, R.P., Powers, A.C., & Stein, R. (2013). Inactivation of specific β cell transcription factors in type 2 diabetes. The Journal of clinical investigation, 123(8), 3305-3316.
8. Tabák, A. G., Herder, C., Rathmann, W., Brunner, E. J., & Kivimäki, M. (2012). Prediabetes: a high-risk state for diabetes development. The Lancet, 379(9833), 2279-2290.
9. Sapra, A., Bhandari, P., & Wilhite Hughes, A. (2021). Diabetes Mellitus (Nursing).
10. Larkin, A. T., Hoffman, C., Stevens, A., Douglas, A., & Bloomgarden, Z. (2015). Determinants of adherence to diabetes treatment: 糖尿病治疗依从性的决定因素. Journal of diabetes, 7(6), 864-871.
11. Vargas-Sánchez, K., Garay-Jaramillo, E., & González-Reyes, R. E. (2019). Effects of Moringa oleifera on glycaemia and insulin levels: A Review of animal and human studies. Nutrients, 11(12), 2907.
12. Maganti AV, Maier B, Tersey SA, Sampley ML, Mosley AL, Özcan S, Pachaiyappan B. Transcriptional activity of the islet β cell factor Pdx1 is augmented by lysine methylation catalyzed by the methyltransferase Set7/9. Journal of Biological Chemistry. 2015 Apr 10;290(15):9812-22.
13. Malenczyk, K., Szodorai, E., Schnell, R., Lubec, G., Szabó, G., Hökfelt, T., & Harkany, T. (2018). Secretagogin protects Pdx1 from proteasomal degradation to control a transcriptional program required for β cell specification. Molecular metabolism, 14, 108-120.
14. Torres J, Mehandru S, Colombel JF, Peyrin-Biroulet L. Crohn's disease. The Lancet. 2017 Apr 29;389(10080):1741-55.
15. Fernández‐Manjarrés JF, Gerard PR, Dufour J, Raquin C, Frascaria‐Lacoste N. Differential patterns of morphological and molecular hybridization between Fraxinus excelsior L. and Fraxinus angustifolia Vahl (Oleaceae) in eastern and western France. Molecular ecology. 2006 Oct;15(11):3245-57..
16. Paikra, B. K., & Gidwani, B. (2017). Phytochemistry and pharmacology of Moringa oleifera Lam. Journal of pharmacopuncture, 20(3), 194.
17. Hinsinger, D. D., Gaudeul, M., Couloux, A., Bousquet, J., & Frascaria-Lacoste, N. (2014). The phylogeography of Eurasian Fraxinus species reveals ancient transcontinental reticulation. Molecular phylogenetics and evolution, 77, 223-237.
18. Zhang, Y.; Liu, D. Flavonol kaempferol improves chronic hyperglycemia-impaired pancreatic beta-cell viability and insulin secretory function. Eur. J. Pharmacol. 2011, 670, 325–332.
19. Stohs, S. J., & Hartman, M. J. (2015). Review of the safety and efficacy of Moringa oleifera. Phytotherapy Research, 29(6), 796-804.
20. Wada, K., Masamune, T., Ino, H., Ueda, K., Ishiyama, T., Sessler, D. I., & Matsukawa, T. (2019). Severe hypoglycemia reduces the shivering threshold in rabbits. BMC anesthesiology, 19(1), 1-6.
21. Nelson EA, Keller GL, Mitchell TW, Pennypacker B, Rebbeck P, Rogers IT. A jugular bleeding technique in rabbits. Lab animal. 2010 Jan;39(1):17-22.
22. Nuhu F. Effect of Moringa leaf meal (MOLM) on nutrient digestibility, growth, carcass and blood indices of weaner rabbits.2010.
23. Dougnon TJ, Aboh BA, Honvou S, Youssao I. Effects of substitution of pellet of Moringa oleifera to commercial feed on rabbit’s digestion, growth performance and carcass trait. Journal of Applied Pharmaceutical Science. 2012 Sep 28;2(9):015-9.
24. El-Wardany I, El-Badawi AY, Helal FI, Ali NG, Aboelazab OM. Growth performance and hematological changes of growing New Zealand White rabbits fed diets supplemented with some natural antioxidants under heat stress conditions. Egyptian Journal of Nutrition and Feeds. 2015 Nov 1;18(2 Special):237-45.
25. Olurishe, C.; Kwanashie, H.; Zezi, A.; Danjuma, N.; Mohammed, B. Chronic administration of ethanol leaf extract of Moringa oleifera Lam. (Moringaceae) may compromise glycaemic efficacy of Sitagliptin with no significant effect in retinopathy in a diabetic rat model. J. Ethnopharmacol. 2016, 194, 895–903.
26. Villarruel-López A, López-De La Mora DA, Vázquez-Paulino OD, Puebla-Mora AG, Torres-Vitela MR, Guerrero-Quiroz LA, Nuño K. Effect of Moringa oleifera consumption on diabetic rats. BMC complementary and alternative medicine. 2018 Dec;18(1):1-0.
27. Udeogu, Ejike, Uzochukwu Amakom, and Shampa Roy-Mukherjee. 2021. Empirical Analysis of an Augmented Schumpeterian Endogenous Growth Model. Journal of Economic Development 46: 53–84.
28. Al-Malki, A.L.; El Rabey, H.A. The Antidiabetic Effect of Low Doses of Moringa oleifera Lam. Seeds on Streptozotocin Induced Diabetes and Diabetic Nephropathy in Male Rats. Biomed. Res. Int. 2015, 2015, 1–13
29. Eldaim, M.A.A.; Elrasoul, A.S.A.; Elaziz, S.A.A.; Shaban, A. An aqueous extract from Moringa oleifera leaves ameliorates hepatotoxicity in alloxan-induced diabetic rats. Biochem. Cell Biol. 2017, 95, 524–530.
30. Zeid W., Hamed M., Mansour N., Diab R. (2020). Prevalence and associated risk factors of self-medication among patients attending El-Mahsama family practice center, Ismailia, Egypt. Bull. Natl. Res. Cent. 44, 92.
Article Sidebar
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
All articles published in JPTCP are licensed under Copyright Creative Commons Attribution-NonCommercial 4.0 International License.
Dr. Shujaullah Talib
MBBS, M.Phil. Assistant Professor Department of Pharmacology Mohammad Medical and Dental College Mirpurkhas.
Dr. Prof. Dr. Syed Mahboob Alam
MBBS, Ph.D. Professor & Head of Pharmacology & Therapeutics BMSI, JPMC, KARACHI
Dr. Qamar Zaman Phull
MBBS MPhil. Assistant Professor Department of Pharmacology Bilawal Medical College for Boys (LUMHS) Jamshoro
Dr. Aziz Ahmed Solangi
Demonstrator/ Lacturer, Pharmacology department, BMC/LUMHS/Jamshoro
Altaf Hussain
Assistant professor in zoology, Government degree college Malir Cantt Karachi Sindh
Mamoona Qureshi
M. Phil Scholar, Faculty of Pharmacy, University of Sindh