ASSESSMENT OF HISTO-PHYSIOLOGICAL AND BIOCHEMICAL CHANGES IN QUAILS (COTURNIX COTURNIX) SUPPLEMENTED BY ZINC AND PROTEXIN REARED UNDER HEAT STRESS
Main Article Content
Keywords
Heat stress, Zinc, growth, performance, biochemical analysiS, histo-physiology.
Abstract
Abstract
This study examined the effects of zinc and protexin supplementation on quails exposed to heat stress (HS). It was revealed that protexin and zinc positively influenced the metabolic parameters of heat-stressed quails, enhancing their overall health and immunity. A total number of one hundred thirty-five (n=135) quails were selected and equally divided into nine groups with each group having 3 replicates (n=5). These groups were designated as T0, T1, T2, T3, T4, T5, T6, T7 and T8 in which T0 was set as control receiving only basal diet. T1 and T2 groups were supplemented with 100mg/kg of zinc+protexin and 150mg/kg of zinc+protexin respectively, without HS. The groups T3, T4, T5 and T6 were exposed to HS and supplemented 100mg/kg zinc, 150mg/kg zinc, 100mg/kg zinc+protexin and 150mg/kg zinc+protexin respectively. While T7 was supplemented with protexin only and T8 was kept only under HS without any supplementation. Results revealed that the birds of HS group had a reduced spleen weight 33.33%, bursa of fabricius 1.56%, liver weight 3.51%, villus width of the jejunum 0.11%, villus length of the jejunum 0.44%, villus length of the ileum 0.35%, Aspartate Aminotransferase (AST) 4.12%, glucose 3.26%, total protein 2.44% and albumin 0.30% concentrations compared to control. Whereas, total cholesterol 1.95%, triglyceride 2.67%, Alanine Aminotransferase (ALT) 10.45%, goblet cell of duodenum 6.75%, goblet cell of jejunum 4.47%, goblet cell of ileum 8.78%, villus width of the duodenum 2.24%, villus length of the duodenum 0.18%, villus width of the ileum 0.22%, intestinal length 10.29% were increased in heat stressed birds as compared to control. Zinc and protexin supplementation reduced the negative effects of heat stress (HS) by increasing spleen weight, glucose, albumin and total protein AST concentrations. The ALT, intestinal length, triglyceride and cholesterol level came back to the normal level with supplementation of zinc and protexin. It was observed that zinc either alone or in combination with protexin mitigated the effects of heat stress on the histo-physiological and biochemical parameters of quails.
References
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Aysha Maqsood
Department of Environmental Sciences, Bahauddin Zakariya University, Multan, Pakistan
Saira Parveen
Department of Environmental Sciences, Bahauddin Zakariya University, Multan, Pakistan
Sadaf Gulzar
Department of Environmental Sciences, Bahauddin Zakariya University, Multan, Pakistan
Maria Gulzar
Department of Pathobiology, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan
Muhammad Usman Saleem
Department of Bio-Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan
Sheraz Ahmed Bhatti
Department of Pathobiology, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan
Abdul Asim Farooq
Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan
Muhammad Arshad Javid
Department of Anatomy & Histology, FAH&VS, Sindh Agriculture University, Tandojam, Pakistan
Muhammad Abdul Basit
Department of Bio-Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan
Murtaz ul Hasan
Department of Parasitology & Microbiology, Faculty of Veterinary and Animal Sciences, PMAS Arid Agriculture University Rawalpindi, Pakistan
Muhammad Nawaz
Department of Environmental Sciences, Bahauddin Zakariya University, Multan, Pakistan