PRECONDITIONING OF STEM CELLS WITH SILVER NANOPARTICLES CAN REDUCE LIVER FIBROSIS IN MICE
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Keywords
Silver, Nanoparticles, Mice, Liver, Plant, Fibrosis
Abstract
Liver fibrosis is the seven largest cause of death worldwide. Human life is very dangerously threatened by advanced liver fibrosis, which has a high morbidity and fatality rate. The only widely approved treatment option for those with severe liver fibrosis is an organ transplant. But due to shortage of donors, immune rejection and high cost of operation have made the liver transplantation process difficult. Therefore, there is a dire need to treat the danger liver disease on time. The aim of this study was to enhance the efficacy of stem cells by preconditioning with biosynthesized silver nanoparticles for the reduction of liver fibrosis. Silver Nanoparticles were extracted from Citrullus Colocynthis leaves and characterized by UV-vis, FTIR, XRD, SEM, and TEM. liver injury was induced in Balb/C mice by four weeks of injection with CCl4. Mice were divided into three groups , Normal, CCl4 control and preconditioning stem cells with selenium nanoparticles treated group. Biochemical such as ALT and Bilirubin, Histopathological of H & E staining and molecular at gene expression levels results showed that preconditioning stem cells with silver nanoparticles is a novel idea treat liver diseases, as these cells have strong hepatoprotective ability to reduce live fibrosis in mice.
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Muhammad Ayaz
Department of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, Khyber Pakhtunkhwa, Pakistan
Maryam Iqbal
Department of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, Khyber Pakhtunkhwa, Pakistan
Sapna Quraish
Department of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, Khyber Pakhtunkhwa, Pakistan
Nazia Imran
Department of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, Khyber Pakhtunkhwa, Pakistan
Huma Rafiq
Department of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, Khyber Pakhtunkhwa, Pakistan
Abbas Khan
Department of Chemistry, Abdul Wali Khan University Mardan, Mardan 23200, Khyber Pakhtunkhwa, Pakistan
Sulaiman Shams
Department of Biochemistry, Abdul Wali Khan University Mardan, Mardan 23200, Khyber Pakhtunkhwa, Pakistan