PHYTOCHEMICAL ESTIMATION AND ANTIOXIDANT ACTIVITY OF VARIOUS EXTRACT OF Stigma maydis (CORN SILK)

Main Article Content

Karuna Singh
Dharmendra Ahuja

Keywords

Phytochemicals, corn silk, solvents, antioxidant activity, free radical scavenging activity

Abstract

The stigmas, or delicate, yellowish threads, from the female flowers of the maize plant are collected to form corn silk. Traditional uses for corn silk include diuretic, antilithiasic, uricosuric and treatment for prostatitis, gout, kidney stones, cystitis, and nephritis. The study examined the phytochemical components, total antioxidant activity, and free radical scavenging ability of many maize silk extracts. Various solvents, including benzene, chloroform, ethanol, ethyl acetate, methanol, and petroleum ether, were used to extract phytochemicals from corn silk. Positive screening results for flavonoids, alkaloids, phenols, steroids, glycosides, carbohydrates, terpenoids, and tannins were obtained from the phytochemical screening process. Methanol was the optimal extract rant for phytochemicals. Because DPPH is a stable antioxidant, its ability to scavenge free radicals was assessed using the DPPH test. Methanol proved to be the most effective solvent for extracting phytochemicals. The assessment of free radical scavenging activity was conducted by employing the DPPH test, which utilizes DPPH as a reliable and stable antioxidant. The methanolic extract exhibited the highest level of DPPH scavenging activity and overall antioxidant activity. The inclusion of flavonoids, alkaloids, phenols, steroids, glycosides, and tannins in maize silk may contribute to these activities.

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References

1. Amar KC, Ghash D and Tripathy S. J Endocrinol Repord 2009; 13(1):17-26.
2. Bako SP, Bakfur MJ, John I and Bela EL. Int J Bot 2005; 1(2):147-150.
3. Bamidele V, Owoyele, Negedu M, Onasanwo SA and Oguntoye SO. J Med Food 2010; 13(2):343-347.
4. BoneDE and Carrington MF. Food Chem 2005; 91:485-494.
5. Ceska O and Styles ED. Phytochem 2011; 23(8):1822-1823.
6. Chandini S K, Ganesan P and Bhaskar N. Food Chem 2008; 107:707-713.
7. Chandra S, Meng J, Zhang Y, Yan J and Li J. J Agri Food Chem 2008; 56(15):6506-6511.
8. Ebrahimzadeh MA, Pourmorad F and Hafezi M. Turk J Bio 2007; 32:43-49.
9. Eman and Alam A. J American Sci 2011; 7(4):726-729.
10. Harborne JB. Phytochemical methods: A guide to Modern Technique of Plant Analysis. Chapman and Hall Ltd., London 1973; 49-188.
11. Jayaprakasha GK, Girennavar B, and Patil BS. Bioresource Tech 2008; 99(10):4484-4494.
12. Liu RH, Mohmoud and Tanabe H. J Nutri 2009; 134:3479-3485.
13. Maksimovic ZA and Kovacevic N. Fitoterapia 2003; 74:144-147.
14. Matkowski A, Tasarz P and Szypula E. J Med Plant 2008; 2(11):321-330.
15. Meenakshi S, Manicka GD, Mozhi TS, Arumugam M and Balasubramanian T. Global J Pharmacol 2009; 3(2):59-62.
16. Mensor LI, Menezes FS, Leitao GG, Reis AS, dos Santos T, Coube CS and Leitao SG. Phytother res 2001; 127-130.
17. Ogunlana OE and Ogunlana OO. Res J Agri Bio Sci 2008; 4(6):666-671.
18. Rajani GP and Ashok P. Ind J Pharmacol 2009; 41(56):227-232.
19. Saez GT, Tormos C, Giner V, Chaves J, Lozano JV, Iradi A and Redon J. Am J hypertension 2007; 17:809-816.
20. CI, K. C., & Indira, G. (2016). Quantitative estimation of total phenolic, flavonoids, tannin and chlorophyll content of leaves of Strobilanthes Kunthiana (Neelakurinji). J. Med. Plants, 4, 282-286.
21. Athavale, A., Jirankalgikar, N., Nariya, P., & Des, S. (2012). Evaluation of In-vitro antioxidant activity of panchagavya: a traditional ayurvedic preparation. Int J Pharm Sci Res, 3, 2543-9.
22. Tangco J.V.V., Angustia D.A., Jelynne P.T. (2015). Nutrional Analysis, Phytochemical Screening & Total Phenolic Content of Basella alba leaves from Philippines. International Journal of Pharmacognosy & Phytochemical research, Philippines, 7(5);1031-1033.
23. Parthasarathy S, Bin Azizi J, Ramanathan S, Ismail S, Sasidharan S, Said MI, et al., (2009) Evaluation of antioxidant and antibacterial activities of aqueous, methanolic and alkaloid extracts from Mitragyna speciosa (Rubiaceae Family) leaves. Molecules 14: 3964-3974.
24. Dasgupta, N., & De, B. (2004). Antioxidant activity of Piper betle L. leaf extract in vitro. Food chemistry, 88(2), 219-224.
25. Quisumbing, E. (1978). Medicinal Plants of the Philippines. Quezon City, Philippines.
26. Phatak, R. S., & Hendre, A. S. (2014). Total antioxidant capacity (TAC) of fresh leaves of Kalanchoe pinnata. Journal of Pharmacognosy and Phytochemistry, 2(5), 32-35