BIOTECHNOLOGICAL APPLICATIONS IN FORENSICS: QUESTIONING THE EFFECT OF SUN LIGHT CONDITIONS ON DNA INTEGRITY EXTRACTED FROM BLOOD AND HAIR SAMPLES
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Keywords
Forensic science, DNA, Forensic biotechnology
Abstract
Biotechnology is deemed to be one of the leading sciences by the fact that is being related to many beneficial applications in our life. One of these applications, is the utilizing the biotechnology in forensic science, which is popularly known as forensic biotechnology, additionally, as the science of biotechnology depends primarily on DNA extraction, it has a vital role in arresting offenders and protecting society. In forensic biotechnology, we deal directly with vital biological evidence such as blood, semen, hair, and various tissues, resulting in imaging of the DNA fingerprint that in the end, leads to capturing the offenders. In conducting our research, we have done several techniques to examine the effect of sunlight conditions on DNA extracts obtained from both, blood and hair samples. In the sake of quantification of the DNA extracts, Nano Drop spectrophotometer device was used to obtain concentrations, 260/280 and 260/230 ratios of the samples, values of 260/280 ratio for fresh blood samples, blood samples kept under sun light conditions and blood samples stored in shadow conditions was 2.342, 1260 and 1.355 respectively. Furthermore, integrity of the DNA samples was determined using agarose gel electrophoresis, random amplified polymorphic DNA and STR analysis, results showed poor and faint bands that referred to all DNA extracts obtained from samples whether exposed to sunlight or not, caused by the low quality and quantity of the DNA samples obtained. Although several former researches proved the effect of the sunlight conditions on DNA, the low quality compared to control samples made it difficult to observe that effect.
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Fayez Althobaiti
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Abdulaziz Lafi T. Alanazi
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Ala’a Ali Abuharbah
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Lojain Abdullah A. Alharbi
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Yara Ali S. Alhussaini
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Ghadi Abdulaziz Alhazmi
Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia