DIAGNOSTIC ACCURACY OF REAL- TIME PCR METHOD IN DETECTION OF CANDIDA ALBICANS IN INFECTED ROOT CANAL
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Keywords
Candida albicans, Infected root canal, Real-time PCR, culture
Abstract
This observational cross-sectional analytical study was conducted in the Department of Conservative Dentistry and Endodontics of Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh to detect candida albicans in root canal samples from endodontic infection. First, samples of infected root canal were taken from 50 patients who required root canal treatment. The carious lesion and coronal restoration were inactivated, the damaged tooth was isolated, and was decontaminated. Without using a chemical solvent, the root canal filling was removed. A radiograph was used to measure and confirm the canal’s length. The microbiology lab received the paper points containing samples for identification of candida albicans by using two diagnostic methods culture and real-time PCR method assessing accuracy of real-time PCR. Candida albicans was isolated in 22 (44%) by real-time PCR on the other hand 10 (20%) by culture. Candida albicans is the most frequent fungus in root canals forming biofilms, regularly found in primary endodontic infections than in persistent infections, in which this yeast is resistant to irrigants and causes endodontic failure.
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Mst. Dil Afroz Bhuiyan
Department of Conservative Dentistry and Endodontics, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka, Bangladesh.
Md. Mujibur Rahman Howlader
Md. Mujibur Rahman Howlader, Department of Conservative Dentistry and Endodontics, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka, Bangladesh.
Elise Shahin Shamsuddin
Department of Conservative Dentistry and Endodontics, Chattagram International Dental College & Hospital, University of Chittagong, Chittagong, Bangladesh.
Asaduzzaman Rakib
Department of Conservative Dentistry and Endodontics, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka, Bangladesh
Mozammal Hossain
Mozammal Hossain, Department of Conservative Dentistry and Endodontics, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka, Bangladesh.