EXPLORATION, ISOLATION, PHENOTYPICAL, & BIOCHEMICAL CHARACTERIZATION OF INDIGENOUS METALLOTOLERANT BACTERIAL ISOLATES FROM HATTAR INDUSTRIAL ESTATE, HARIPUR: TOWARDS ENVIRONMENTAL REMEDIATION AND SUSTAINABLE INDUSTRIAL PRACTICES
Main Article Content
Keywords
Metallotolerant bacteria, Heavy metal contamination, Bioremediation, Environmental health, Sustainable industrial
Abstract
This study addresses heavy metal contamination in Hattar Industrial Estate, Haripur, Pakistan, emphasizing the global issue of environmental pollution. The research focuses on isolating and characterizing metallotolerant bacterial strains from the contaminated soil, aiming to contribute to sustainable industrial practices and environmental health. Soil samples from Hattar Industrial Estate were collected and metallotolerant bacteria were isolated using a serial dilution method. two strains (Strain A, Strain B) were selected for further analysis based on their resistance to heavy metals. Morphological and biochemical characterization, including catalase, citrate, indole, urease, coagulase, and oxidase tests, were performed to understand the strains' traits and metabolic capabilities. The isolated strains, identified as Kingella sp., Listeria sp., demonstrated distinct morphological characteristics. Biochemical tests revealed variations in catalase, citrate utilization, indole production, urease activity, coagulase reaction, and oxidase activity among the strains. The results indicated the strains' potential for biological remediation, with specific resistance to heavy metals. The study underscores the importance of bioremediation as an eco-friendly approach to mitigate heavy metal contamination. The isolated bacterial strains, resistant to various heavy metals, hold promise for sustainable remediation strategies. The research advocates for the application of these strains in addressing heavy metal pollution, contributing to environmental sustainability, and promoting eco-friendly practices in industrial areas.
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Ayisha Bibi
Department of Microbiology, Hazara University, Mansehra - Pakistan
Abdul Ghafar Khan
Mohi-Ud-Din Islamic University Nerian Sharif - AJ&K
Sumaira Noor
State K ey Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 21003 - China
Sadia Ikram
Department of Microbiology/Pathology, HBS Medical and Dental College, Islamabad - Pakistan
Institute of Molecular Biology and Biotechnology (IMBB), University of Lahore - Pakistan
Saima Inam
Department of Microbiology/Pathology, Sharif Medical and Dental College, Lahore - Pakistan
Institute of Molecular Biology and Biotechnology (IMBB), University of Lahore - Pakistan
Syed Zeeshan Haider
Institute of Molecular Biology and Biotechnology (IMBB), University of Lahore - Pakistan
Attiq Ullah
College of Life Sciences, Northeast Forestry University, Harbin 150040 - China
Sania Javed
Faculty of Biomedical and Health Sciences, Department of Microbiology, University of Haripur - Pakistan