PHYTOCHEMICAL ANALYSIS, IN-VITRO AND IN-VIVO PHARMACOLOGICAL EVALUATION OF DRYOPTERIS JUXTAPOSITA CHRIST (FRONDS)
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Keywords
Dryopteris Juxtaposita Christ, Anti-depressant, Antibacterial activity, Phytochemical Screening
Abstract
Natural product research is one of the keys towards the development of pharmaceuticals. Due to the lack of understanding of the causes of many human, animal, and plant diseases, these challenging chemical compounds have the potential to be active therapeutics. In this research, a frond of the plant was collected in Shangla District, Khyber Pakhtunkhwa during May. It was identified as Dryopteris Juxtaposita Christ, Fronds are used locally to treat stomach ulcers, bone problems, constipation and also in vegetables. Shade-dried plant material was pulverized and extracted into methanol. It was then subfractionated into aqueous, ethyl acetate-hexane and chloroform fractions. Preliminary phytochemical investigation was done to find the primary and secondary metabolites in the sample. The organic extract was tested for antidepressant and antibacterial activity. The crude extract had very little effect compared to regular imipramine in the forced swimming test to evaluate antidepressant efficacy. Additionally, the fractions of the crude extract showed a certain amount of antibacterial activity. When it came to Salmonella typhi, the ethyl acetate fraction had the most activity (60%) and Escherichia coli, the n-hexane fraction had the highest activity (46.43%). Other fractions, though, showed minimal antimicrobial activity. Phytochemical analysis of the crude extract of the Fronds of Dryopteris Juxtaposita Christ revealed the presence of a high concentration of amino acids, proteins, tannins, saponins and cardiac glycosides in all extracts/fractions. Whereas, no lipids but trace amounts of steroids were detected in these extracts/fractions. Based on the appreciable activities this plant should be further studied to isolate and identify additional bioactive compounds. To determine possible In-vivo effects of different isolated compounds having potent antioxidant, cytotoxic activities and anticancer activities against known cancer cell lines. This will lead to the development of safe pharmaceuticals.
References
2. Ramaiah AK, Garampalli RKHJAJoPS, Research. In vitro antifungal activity of some plant extracts against Fusarium oxysporum f. sp. lycopersici. Asian Journal of Plant Science and Research. 2015; 5(1):22-27.
3. Subarnas A, Tadano T, Nakahata N, et al. A possible mechanism of antidepresant activity of beta-amyrin palmitate isolated from lobelia inflata leaves in the forced swimming test. Life Sciences. 1993; 52(3):289-296.
4. Aladeokin AC, Umukoro SJJonm. Psychopharmacological properties of an aqueous extract of Tetracarpidium conophorum Hutch. & Dalziel in mice. Journal of natural medicines. 2011; 65:411-416.
5. Oloya B, Namukobe J, Ssengooba W, Afayoa M, Byamukama RJTm, health. Phytochemical screening, antimycobacterial activity and acute toxicity of crude extracts of selected medicinal plant species used locally in the treatment of tuberculosis in Uganda. Tropical medicine and health. 2022;50(1):1-13
6. Muhammad N, Saeed M, Khan HJBc, medicine a. Antipyretic, analgesic and anti-inflammatory activity of Viola betonicifolia whole plant. BMC complementary and alternative medicine. 2012; 12:1-8.
7. Sofowora AJSBl. Medicinal plant and traditional medicine in Africa. Ibadan-Owerri-Kaduna-Lagos. Spectrum Book ltd. 1993:158.
8. Bokhari J, Khan MR, Haq IUJT, health i. Assessment of phytochemicals, antioxidant, and anti-inflammatory potential of Boerhavia procumbens Banks ex Roxb. Toxicology and industrial health. 2016; 32(8):1456-1466.
9. Tosun M, Ercisli S, Sengul M, Ozer H, Polat T, Ozturk EJBR. Antioxidant properties and total phenolic content of eight Salvia species from Turkey. Biological Research. 2009;42(2):175-181.
10. Sharififar F, Dehghn-Nudeh G, Mirtajaldini MJFc. Major flavonoids with antioxidant activity from Teucrium polium L. Food Chemistry. 2009; 112(4):885-888.
11. Ali MS, Mostafa K, Raihan MO, Rahman MK, Aslam MJIjddr. Antioxidant and Cytotoxic activities of Methanolic extract of Dryopteris filix-mas (L.) Schott Leaves. International Journal of Drug Development & Research. 2012; 4:223-229.
12. Zhao D-D, Zhao Q-S, Liu L, et al. Compounds from Dryopteris fragrans (L.) Schott with cytotoxic activity. Molecules. 2014; 19(3):3345-3355.
13. Erhirhie EO, Emeghebo CN, Ilodigwe EE, et al. Dryopteris filix-mas (L.) Schott ethanolic leaf extract and fractions exhibited profound anti-inflammatory activity. Avicenna journal of phytomedicine. 2019;9(4):396.
14. Khan H, Khan MA, Muhammad N, Ashraf N, Gul F, Tariq SAJP. Antiinflammatory and antioxidant activity of Joshanda partially mediated through inhibition of lipoxygenase. Phytopharmacology. 2012;3:19-28.
15. Abdelhalim A, Karim N, Chebib M, et al. Antidepressant, anxiolytic and antinociceptive activities of constituents from Rosmarinus officinalis. Journal of Pharmacy & Pharmaceutical Sciences. 2015;18(4):448-459.
16. Harborne J. Phytochemical methods London Chapman and Hall. Ltd; 1973.
17. Manas B, Rajesh Y, Kumar VR, Praveen B, Mangamma KJIJop, sciences b. Extraction, phytochemical screening and in-vitro evaluation of anti-oxidant properties of Commicarpus chinesis (aqueous leaf extract). International Journal of Pharma and Bio Sciences. 2010;1(4)
18. Zhang T, Wang L, Duan D-H, Zhang Y-H, Huang S-X, Chang YJM. Cytotoxicity-guided isolation of two new phenolic derivatives from Dryopteris fragrans (L.) Schott. Molecules. 2018; 23(7):1652.
19. Obianime A, Uche FJJoAS, Management E. The Phytochemical screening and the effects of methanolic extract of Phyllanthus amarus leaf on the Biochemical parameters of Male guinea pigs. Journal of Applied Sciences and Environmental Management. 2008;12(4)
20. Abdallah EM, Abdalla WEJJBMOA. Black pepper fruit (Piper nigrum L.) as antibacterial agent: A mini-review. Journal of Bacteriology & Mycology. 2018;6(2):141-145. DOI: 10.15406/jbmoa.2018.06.00192
21. Araujo RC, Neves FA, Formagio AS, et al. Evaluation of the anti-mycobacterium tuberculosis activity and in vivo acute toxicity of Annona sylvatic. BMC Complement Altern Med 14. 2014; 14:1-10. https://doi.org/10.1186/1472-6882-14-209
22. NCCLS (National Committee for Clinical Laboratory Standards), 2002. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard. National Committee for Clinical Laboratory Standards, Wayne, Pennsylvania,USA
23. Sher H, Ali A, Ullah Z, Sher HJER, Applications. Alleviation of Poverty through Sustainable Management and Market Promotion of Medicinal and Aromatic Plants in Swat, Pakistan: Alleviation of Poverty through Sustainable Management. Ethnobotany Research and Applications. 2022;23:1-19
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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Obaid Ullah
Department of Agricultural Chemistry & Biochemistry, Faculty of Nutrition Sciences, The University of Agriculture, Peshawar-Pakistan.
Mudassar Iqbal
Department of Agricultural Chemistry & Biochemistry, Faculty of Nutrition Sciences, The University of Agriculture, Peshawar-Pakistan.
Abdur Rauf
Department of Chemistry, University of Swabi, Anbar-23561, Khyber Pakhtunkhwa, Pakistan
W. ThomasShier
Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN, USA