TO EVALUATE THE ANTIOXIDANT ACTIVITY OF ETHANOLIC EXTRACT OF ALOE BARBADENSIS (EEAB), SALIX TETRASPERMA (EEST) AND TENACETUM PARTHENIUM (EETP) LEAVES.
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Keywords
invitro antioxidant assay, ethanolic extract, DPPH assay, Superoxide scavenging assay
Abstract
Aim: To evaluate the antioxidant activity of Ethanolic extract of Aloe barbadensis (EEAB), Salix tetrasperma (EEST) and Tenacetum parthenium (EETP) leaves
Method: The crude drug was subjected to pharmacognostical evaluation and standardization for confirmation of its purity, then alcoholic extract of all these plants was subjected to qualitative phytochemical analysis and quantitatvely it was idnetified for total phenolic and flavanoid content. In vitro anti oxidant activity was further evaluated by using DPPH scavenging assay, reducing power assay and Superoxide scavenging activity and IC50 value was calculated.
Results: In this investigation, the in-vitro antioxidant effect of AB, TP and ST extract on DPPH radical scavenging activity of AB, TP and ST ethanol extract exhibited percent inhibition 83.39, 76.71 and 77.98% and its IC50 value were found to be 25.97, 42.28, and 47.17μg/ml. Ascorbic acid was used as a reference compound which exhibited percent inhibition 82.61% and showed IC50 value of 18.08 μg/ml. The AB, TP and ST ethanol extract displayed SOS activity which exhibited percent inhibition of 78.38, 6.17 and 71.67% and showed IC50 value of 29.95, 47.87 and 54.17μg/ml. Similarly, for SOS activity, Ascorbic acid was used as a reference compound which exhibited percent inhibition 85.59% and showed IC50 value of 14.6μg/ml.
Conclusion: The reducing capacity of a compound may serve as a significant indicator of its potential antioxidant activity. Dietary antioxidant such as ascorbic acid was used for comparison. Compounds with reducing power indicate that they are electron donors and can reduce the oxidized intermediates of lipid peroxidation processes, so that they can act as primary and secondary antioxidants. The ethanolic extract of Aloe barbadensis showed strong reducing capacity as compared to other extract
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Dinesh Kumar
Research Scholar, Faculty of Pharmaceutical Science, Jayoti Vidyapeeth Women’s University, Jaipur- 303122, Rajasthan, INDIA
Ahuja Dharmendra
Dean & Director, Faculty of Pharmaceutical Science, Jayoti Vidyapeeth Women’s University, Jaipur- 303122, Rajasthan, INDIA