COMPARISON OF THE CELLULAR COMPOSITION OF VARIOUS PLATELET-RICH PLASMA PREPARATIONS
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Keywords
platelet-rich plasma, preparations, cell composition
Abstract
Purpose of the study is to evaluate variations in formulations used to prepare platelet-rich plasmas (PRPs) result in differences in the cellular composition and biomolecular characteristics. Study design was controlled laboratory study. In this study five preparation procedures were performed for 14 healthy subjects, including 2 manual procedures (single-spin at 900g for 5 minutes; double-spin at 900g for 5 minutes and then 1500g for 15 minutes) and 3 methods with commercial kits (Arthrex ACP, Biomet GPS, and Prodizen Prosys). Results of the study showed that the DS PRP had a higher concentration of platelets and leukocytes than did the SS PRP. Every PRP preparation exhibited an increase in PDGF, TGF, VEGF, and FGF release when compared with whole blood samples. In short word the DS method generally led to a higher concentration of platelet relative to the SS method.
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Barkat Ali
Department of Pharmacology, Faculty of Pharmacy, Ziauddin University
Zahida Memon
Department of Pharmacology, Faculty of Health Sciences, Ziauddin University
Mirza Tasawer Baig
Department of Pharmacy Practice, Faculty of Pharmacy, Ziauddin University
Muhammad Latif
Department of Orthopedic, Sindh Govt. Hospital Laiquatabad
Maryam Ahmed
Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi
Syed Zia ul Hasnain
Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi
Aisha Jabeen
Department of Pharmacology, Faculty of Pharmacy, Ziauddin University