APOLIPOPROTEIN B rs1042031 POLYMORPHISM AND LIPID PROFILE OF CORONARY ARTERY DISEASE IN THE PAKISTANI POPULATION
Main Article Content
Keywords
Apolipoprotein B, genetic polymorphism, lipid profile, RFLP
Abstract
Apolipoprotein B (APOB) is linked to atherosclerosis emergence and Coronary Artery disease (CAD) pathophysiology. Single nucleotide polymorphism (SNP) in the APOB gene influences disease susceptibility. This study aimed to determine the association of rs1042031 and serum levels of lipids on the risk of CAD in a population from Pakistan. A cross-sectional study of 200 subjects was conducted at the University of Health Sciences, Lahore. Angiographically diagnosed cases of CAD were recruited from the Punjab Institute of Cardiology, Lahore, during 2021-2022. The lipid profile was done through the calorimetric method. Genotyping was done through Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The frequency of AA genotype was significantly higher in CAD cases 98 (72.6%) as compared to non-CAD group 4 (6.2%) (p=0.018). The adjusted odds ratio for the AA genotype was 2.09 and 0.937 for the GA genotype. Compared with the lipid profile, a significant increase in cholesterol, serum LDL, and total lipids was observed for the AA genotype in the CAD group (p<0.001).
Conclusion: The AA genotype of the APOB rs1042031 gene is a risk factor for CAD in the Pakistani population.
References
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Hira Sohail
Institute of Molecular Biology and Biotechnology, The University of Lahore, Lahore; Pakistan
Madeeha Shahzad
Insitute of Molecular Biology and Biotechnology, The University of Lahore, Lahore; Pakistan
Saba Khaliq
University of Health Sciences, Lahore; Pakistan
Farhat Ijaz
CMH Lahore Medical College and Institute Of Dentistry, Lahore; Pakistan
Qurban Ali
Department of Plant Breeding and Genetics, University of the Punjab, Lahore, Pakistan
Muhammad Arshad Javed
Department of Plant Breeding and Genetics, University of the Punjab, Lahore, Pakistan
Peng Jin-liang
Department of Emergency, The Affiliated Ganzhou Hospital of Nanchang University,341000 Ganzhou, Jiangxi Province, P.R. China
Qiu Chong-Rong
Department of Emergency, The Affiliated Ganzhou Hospital of Nanchang University,341000 Ganzhou, Jiangxi Province, P.R. China