EVALUATION OF APICAL EXTRUSION OF INTRACANAL BIOFILM AFTER ROOT CANAL PREPARATION USING THREE DIFFERENT ROTARY INSTRUMENTATION SYSTEMS: AN IN VITRO STUDY
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Keywords
Apical Extrusion, Intracanal, Biofilm, Root Canal, Rotary Instrumentation, In Vitro Study
Abstract
Background- Endodontic instrumentation carries the risk of extrusion of debris and bacteria. The instrumentation technique used and the type of instrumentation influences this risk of extrusion.
Aim- The purpose of this study was to evaluate and compare the apical extrusion of intracanal biofilm after root canal preparation using Hyflex CM, K3XF and Heroshaper.
Objective- 1)To evaluate the amount of bacteria extruded apically after root canal preparation using Hyflex CM.
2)To evaluate the amount of bacteria extruded apically after root canal preparation using K3XF.
3)To evaluate the amount of bacteria extruded apically after root canal preparation using Heroshaper.
4)To compare the amount of bacteria extruded apically after root canal preparation using Hyflex CM, K3XF, Heroshaper.
Methodology- Sixty five extracted single-rooted human mandibular premolar teeth were used. Endodontic access cavities were prepared and root canals were then contaminated with a suspension of Enterococcus faecalis and dried. Re-inoculation process was done upto 30 days.The contaminated roots were divided into five groups. Group 1=Hyflex CM,Group 2=K3xf,Group 3=Heroshaper,Group 4= Negative Control Group,Group 5= Positive Control Group.Before instrumentation positive control group was checked by SEM.Group 1,2 and 3 were instrumented upto #25,4%.Bacteria extruded from the apical foramen during instrumentation were collected into vials.Samples were incubated in brain heart incubation agar at 370 C for 24 hr.The resulting bacterial titre ,in colony-forming units(CFU) per ml was determined. The data obtained were analysed using the Analysis of variance (ANOVA) Test and post hoc analysis.
Results- Heroshaper extruded least amount of bacteria followed by K3XF files. Maximum extrusion of E. faecalis was seen in hyflex CM group.
Conclusion- All the rotary instruments extruded bacteria apically after root canal preparation.
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Farheen Akhtar
Post Graduate, Department of Conservative dentistry and Endodontics, Hitkarini Dental College, Jabalpur, M.P, India.
Bonny Paul
Professor, Department of Conservative dentistry and Endodontics, Hitkarini Dental College, Jabalpur, M.P, India
Mantri Shivkumar
Professor and Head, Department of Conservative dentistry and Endodontics, Hitkarini Dental College, Jabalpur, M.P, India.
Kavita Dube
Professor, Department of Conservative dentistry and Endodontics, Hitkarini Dental College, Jabalpur, M.P, India.
Ankita
Post Graduate, Department of Conservative dentistry and Endodontics, Hitkarini Dental College, Jabalpur, M.P, India.