EVALUATION OF CARDIOSPERMUMHALICACABUM LINN. ETHANOLIC EXTRACT FOR ANTIOXIDANT AND ANTICANCERACTIVITY
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Keywords
Cardiospermum halicacabum, antioxidant (DPPH scavenging activity), anticanceractivity (MTT Assay), L929 and PC-3 cell line
Abstract
PlantclimbingCardiospermumhalicacabumLinn.Cardiospermumhalicacabumvar.and
Cardiospermum microcarpumare synonyms forCardiospermum halicacabumLinn. The lesserballoon vine, balloon plant, love in a puff, heart seed vine, and heart pea are other names for it. Itsgrowthtypeisaperennialherbaceousvinewithslender,downystemsthatclimbswithtendrilsupto 3m on a support. The monoecious flower species has both male and female flowers on the sameplant. In its native surroundings, this species can be found in grasslands, shrublands, forest margins,wastelands,andcultivatedareas. The herb is used to cure rheumatism, abdominal pain, orchitis,skindisorders,cough,andhyperthermiaintraditionalIndianmedicine.CardiospermumhalicacabumL. whole plant ethanolic extracts were tested for their ability to scavenge DPPH atdoses of 20µg, 40µg, 60µg, 80µg, and 100µg. The results suggest that 57µg is the half maximuminhibitoryconcentration(IC50).Cardiospermum halicacabumL. whole plant ethanolic extractswere tested for anticancer activity using the MTT assay at doses of 5µg, 25µg, 50µg, 75µg, and100µg.Cardiospermum halicacabumL., the sample given, exhibited mild to moderate cytotoxicityto L929 cell line, mild to severe cytotoxicity to PC-3 cells . As would be predicted, the controlrevealed no cytotoxicity. The results suggest the ethanolic extract possesses an cytotoxic propertywith an IC50 values of 75 µg for normal cell lines and IC50 value of 22 µg for prostate cancer celllines.Thus,wereachtheconclusion that the whole plant ethanolic extract ofCardiospermumhalicacabumL.is asuperiorcrude medicineofchoice forantioxidantand anticanceractivity.
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