Investigation of in vitro cytocompatibility of Annona Muricata selenium nanoparticles (SeNps) and its impact on cell migration in human Gingival Fibroblast cells
Main Article Content
Keywords
Annona muricata, cytotoxicity, selenium nanoparticles, Cell proliferation, Apoptosis
Abstract
Background: Annona muricata has been used to treat a wide range of illnesses, making it a crucial species for ethnomedicine. Different parts of A. muricata are used in developing an alternative medicinal extract. Interestingly over the past few decades, metallic nanoparticles have captured the attention of scientists, and selenium is an essential component of amino acids. Hence in the present study A. muricata extracts coated with selenium particles (SeNps) were used to analyze the cytotoxicity of the extract in the gingival fibroblast cells.
Methods: Periodontal ligament tissues were taken from healthy adolescent patients' interdental papillae and the primary culture of gingival fibroblast was established. After two to three passages, the cells were treated with A. muricata SeNps. The cell proliferation, migration, and apoptosis assays were performed before and after treatment with A. muricata SeNps.
Results and Conclusion: The morphology of the isolated fibroblast were in good condition and the cytotoxicity assays revealed that the proliferation and migration increased on treatment with A. muricata SeNps while the apoptosis was less. Thus the findings from the current study proved that A. muricata SeNps had less cytotoxic effects and could be used as a potential therapeutic compound in human diseases.
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