Assessment Of Antifungal and Cytotoxicity On Aqueous Solution Of Acai Berry With Probiotics Against Candida albicans

Abstract

Aim: To assess antifungal and cytotoxic effects of acai berry with probiotics.
Materials and methods: Six millilitres of rose-bengal broth were produced, sterilised, and added to three test tubes. In the range of “5*10 billion cfu/ml”, Candida suspension was applied to each of the three test tubes. the incubation period of 1, 2, 3, or 4 hours. To determine the lowest inhibitory activity, the percentage of dead cells is then computed at a “wavelength of 600 nm”. “200 ml of distilled water” were used to dissolve “2g of iodine-free salt” to test for cytotoxicity. We filled “6 well Elisa plates” with “10–12 cc of saline water”. The combined acai berry and probiotics were then added in accordance with the concentration levels after 10 nauplii were gradually added to each well (“20 l, 40 l, 60 l, 80 l, and 100 l, plus control”). 24 hours were spent incubating the plates.
Results: The results are calculated by day 1and day 2. On day 1, at concentration of 5μl,10μl,20μl,40μl,80μl and the control group the number of nauplii alive was 10. On day 2, at concentration of 5μl 8 nauplii alive, at 10μl 8nauplii was alive,20μl 7 nauplii was alive, 40μl 7 nauplii was alive, 80μl 6 nauplii was alive.
Conclusion: Result shows the acai berry and probiotics formations shows antifungal property and good cytotoxic effect, in future can be used in management of oral candidiasis associated lesion.