Assessment of the antimicrobial effect of a novel Chitosan-Sunflower seed gel – An in vitro study
Main Article Content
Keywords
Chitosan Gel, Sunflower seed extract, antimicrobial efficacy
Abstract
Background: The development of multidrug resistant microbes has led to increasing interest towards natural products with antibacterial properties. 2 such products are chitosan and sunflower seed oil. Given the importance of discovering novel treatments for patients with wounds, the goal of this study was to assess the antibacterial activity of a novel chitosan-sunflower seed gel in vitro.
Materials and Method: A novel chitosan-sunflower seed gel was prepared. The antimicrobial activity of the prepared gel, in three different concentrations (25, 50 and 100μl), was tested by Kirby-Bauer disc diffusion method against 5 different microbes: Staphylococcus aureus, Candida albicans, Lactobacillus, Enterococcus faecalis and Streptococcus mutans. The inhibition zones were measured using a vernier calliper.
Result: The 100μl dilution of the gel had the best antimicrobial activity against all the tested microorganisms. The antimicrobial activity against Candida albicans was good and was comparable to that of the control antibiotic used (83%), followed by that against Enterococcus faecalis (66.66%), Streptococcus mutans (64%), Staphylococcus aureus (50%) and Lactobacillus (40%) at the 100μl dilution.
Conclusion: The chitosan-sunflower seed gel is a new and propitious alternative with antimicrobial properties against the tested microbial agents. Further studies in a clinical setting are warranted to corroborate the in vitro results of this study so that appropriate treatment protocols can be established.
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12. Isoshima D, Yamashiro K, Matsunaga K, Shinobe M, Nakanishi N, Nakanishi I, et al. Assessment of pathogenesis of infective endocarditis by plasma IgG antibody titer test against periodontal bacteria. Clin Case Rep. 2017 Oct;5(10):1580–6.
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16. J PC, Marimuthu T, C K, Devadoss P, Kumar SM. Prevalence and measurement of anterior loop of the mandibular canal using CBCT: A cross sectional study. Clin Implant Dent Relat Res. 2018 Aug;20(4):531–4.
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19. Rodrigues HG, Vinolo MAR, Sato FT, Magdalon J, Kuhl CMC, Yamagata AS, et al. Oral Administration of Linoleic Acid Induces New Vessel Formation and Improves Skin Wound Healing in Diabetic Rats. PLoS One. 2016 Oct 20;11(10):e0165115.
20. Siddique R, Sureshbabu NM, Somasundaram J, Jacob B, Selvam D. Qualitative and quantitative analysis of precipitate formation following interaction of chlorhexidine with sodium hypochlorite, neem, and tulsi. J Conserv Dent. 2019 Jan-Feb;22(1):40–7.
21. Lezcano I, Nuñez N, Espino M, Gómez M. Antibacterial Activity of Ozonized Sunflower Oil, Oleozón, AgainstStaphylococcus aureusandStaphylococcus epidermidis [Internet]. Vol. 22, Ozone: Science & Engineering. 2000. p. 207–14. Available from: http://dx.doi.org/10.1080/01919510008547221
22. Montevecchi M, Marucci G, Dorigo A, Checchi L. Permanent mucosal discoloration correlated to magnesium-enriched hydroxyapatite: A case report [Internet]. Vol. 30, Dental Materials. 2014. p. e97. Available from: http://dx.doi.org/10.1016/j.dental.2014.08.198
23. Geweely N. Synthesis, structural characterization, anticandidal, antitumor activities and cell wall modulation of novel potentially active nitrogen–oxygen donor ligand
and its metal (II) complexes against some pathogenic yeasts [Internet]. Vol. 25, New Biotechnology. 2009. p. S87. Available from: http://dx.doi.org/10.1016/j.nbt.2009.06.356
24. Daud FV, Ueda SMY, Navarini A, Mímica LMJ. The use of ozonized oil in the treatment of dermatophitosis caused by Microsporum canis in rabbits [Internet]. Vol. 42, Brazilian Journal of Microbiology. 2011. p. 274–81. Available from: http://dx.doi.org/10.1590/s1517-83822011000100035
25. Valacchi G, Zanardi I, Lim Y, Belmonte G, Miracco C, Sticozzi C, et al. Ozonated oils as functional dermatological matrices: Effects on the wound healing process using SKH1 mice [Internet]. Vol. 458, International Journal of Pharmaceutics. 2013. p. 65–73. Available from: http://dx.doi.org/10.1016/j.ijpharm.2013.09.039
26. Manhezi AC, Bachion MM, Pereira AL. [The use of essential fatty acids in the treatments of wounds]. Rev Bras Enferm. 2008 Sep;61(5):620–8.
27. Vijayakumar Jain S, Muthusekhar MR, Baig MF, Senthilnathan P, Loganathan S, Abdul Wahab PU, et al. Evaluation of Three-Dimensional Changes in Pharyngeal Airway Following Isolated Lefort One Osteotomy for the Correction of Vertical Maxillary Excess: A Prospective Study. J Maxillofac Oral Surg. 2019 Mar;18(1):139–46.
28. Nandakumar M, Nasim I. Comparative evaluation of grape seed and cranberry extracts in preventing enamel erosion: An optical emission spectrometric analysis. J Conserv Dent. 2018 Sep-Oct;21(5):516–20.
29. Leuba JL, Stossel P. Chitosan and Other Polyamines: Antifungal Activity and Interaction with Biological Membranes [Internet]. Chitin in Nature and Technology. 1986. p. 215–22. Available from: http://dx.doi.org/10.1007/978-1-4613-2167-5_29
30. Moraes PC de, de Moraes PC, de Souza Marques IC, Basso FG, Rossetto HL, de Carvalho Panzeri Pires-de-Souza F, et al. Repair of Bone Defects with Chitosan-Collagen Biomembrane and Scaffold Containing Calcium Aluminate Cement [Internet]. Vol. 28, Brazilian Dental Journal. 2017. p. 287–95. Available from: http://dx.doi.org/10.1590/0103-6440201601454
31. Zivanovic S, Li J, Michael Davidson P, Kit K. Physical, Mechanical, and Antibacterial Properties of Chitosan/PEO Blend Films [Internet]. Vol. 9, Biomacromolecules. 2008. p. 1355–1355. Available from: http://dx.doi.org/10.1021/bm800180k
32. Kamjumphol W, Chareonsudjai P, Chareonsudjai S. Antibacterial activity of chitosan against Burkholderia pseudomallei. Microbiologyopen [Internet]. 2018 Feb;7(1). Available from: http://dx.doi.org/10.1002/mbo3.534
33. D’Almeida M, Attik N, Amalric J, Brunon C, Renaud F, Abouelleil H, et al. Chitosan coating as an antibacterial surface for biomedical applications [Internet]. Vol. 12, PLOS ONE. 2017. p. e0189537. Available from: http://dx.doi.org/10.1371/journal.pone.0189537
34. Zheng LY, Zhu JF. Study on antimicrobial activity of chitosan with different molecular weights [Internet]. Vol. 54, Carbohydrate Polymers. 2003. p. 527–30. Available from: http://dx.doi.org/10.1016/j.carbpol.2003.07.009
35. Hussainy SN, Nasim I, Thomas T, Ranjan M. Clinical performance of resin-modified glass ionomer cement, flowable composite, and polyacid-modified resin composite in noncarious cervical lesions: One-year follow-up. J Conserv Dent. 2018 Sep-Oct;21(5):510–5.
36. Kachhara S, Nallaswamy D, Ganapathy DM, Sivaswamy V, Rajaraman V. Assessment of intraoral scanning technology for multiple implant impressions - A systematic review and meta-analysis. J Indian Prosthodont Soc. 2020 Apr 7;20(2):141–52.
37. Merchant A, Ganapathy DM, Maiti S. Effectiveness of local and topical anesthesia during gingival retraction: Anesthesia during cord packing. Brazilian Dental Science [Internet]. 2022; Available from: https://bds.ict.unesp.br/index.php/cob/article/download/2591/4477
38. Abhay SS, Ganapathy D, Veeraiyan DN, Ariga P, Heboyan A, Amornvit P, et al. Wear Resistance, Color Stability and Displacement Resistance of Milled PEEK Crowns Compared to Zirconia Crowns under Stimulated Chewing and High-Performance Aging. Polymers [Internet]. 2021 Oct 30;13(21). Available from: http://dx.doi.org/10.3390/polym13213761
39. Jain AR, Dhanraj M. A clinical review of spacer design for conventional complete denture. Early Pregnancy. 2016;8(5):1.
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Jun 1, 2023
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Ojasvini Arora, Subhashree Rohinikumar, S Rajeshkumar, Nabeel Ahmed, & Dhanraj Ganapathy. (2023). Assessment of the antimicrobial effect of a novel Chitosan-Sunflower seed gel – An in vitro study. Journal of Population Therapeutics and Clinical Pharmacology, 30(12), 160-167. https://doi.org/10.47750/jptcp.2023.30.12.018
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How to Cite
Ojasvini Arora, Subhashree Rohinikumar, S Rajeshkumar, Nabeel Ahmed, & Dhanraj Ganapathy. (2023). Assessment of the antimicrobial effect of a novel Chitosan-Sunflower seed gel – An in vitro study. Journal of Population Therapeutics and Clinical Pharmacology, 30(12), 160-167. https://doi.org/10.47750/jptcp.2023.30.12.018