ANTICANCER ACTIVITY OF HYDRO-ALCOHOLIC EXTRACTS OF FINGER MILLET AGAINST BREAST AND LUNG CANCER CELL LINES
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Keywords
finger millet, fermentation, GC-MS screening, breast cancer, lung cancer
Abstract
Globally cancer is the most public health concern and a common cause of death among all age groups, even with the availability of chemotherapeutic, radiation and surgical interventions. Phytochemicals are known for their anti-inflammatory, antioxidant and cancer-inhibitory activities. In the present study, GC-MS screening of finger millet extract was done along with impact assessment of methanolic and aqueous methanolic extracts of raw and fermented finger millet grains flour against breast and lung cancer cell lines (MTT assay) at various doses. GC-MS screening revealed the presence of phytosterols. The MTT assay confirmed the anticancer activity of finger millet extracts at 100, 200 and 400 µg/ml in a dose-dependent manner. However, the extract of the fermented samples prepared from aqueous methanol showed the highest cytotoxicity. So, there is a need for HPLC quantification of the screened phytochemicals and dose optimization for safety assessment. Furthermore, fermented samples should also be screened for compound identification and quantification.
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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Muhammad Amir
Department of Food Science and Technology, Faculty Food Science and Nutrition, Bahauddin Zakariya University, Multan, Pakistan.
Saeed Akhtar
Department of Food Science and Technology, Faculty Food Science and Nutrition, Bahauddin Zakariya University, Multan, Pakistan.
Tariq Ismail
Department of Food Science and Technology, Faculty Food Science and Nutrition, Bahauddin Zakariya University, Multan, Pakistan.
Wisha Saeed
Department of Food Science and Technology, Faculty Food Science and Nutrition, Bahauddin Zakariya University, Multan, Pakistan.
Tahir Maqbool
Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan.
Zeeshan Haider Naqvi
Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan.
Muhammad Naeem Zubairi
Government of Pakistan, Islamabad Capital Territory, Islamabad Food Authority.