PROSTATE CANCER IN SOUTHWEST PAKISTAN: MOLECULAR DETECTION BY METHYLATION-SPECIFIC PCR FROM URINE SPECIMENS
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Keywords
Prostate cancer, Benign prostatic hyperplasia, Methylation-specific polymerase chain reaction, Glutathione S-transferase P1
Abstract
Introduction: Prostate cancer (PCa) represents the second most prevalent malignancy among males, which is characterized by a high mortality rate. The aim of our study was to evaluate the methylation status of glutathione S-transferase P1 (GSTP1) in urine specimens from males with PCa and benign prostatic hyperplasia (BPH) and its usefulness in distinguishing between males with PCa and BPH by non-invasive methods.
Methodology: Voided urine specimens were collected from 78 patients with PCa and 57 patients with BPH. Genomic DNA was isolated and subjected to bisulfite modification. Methylation status of the GSTP1 gene was determined by conventional methylation-specific polymerase chain reaction (MSP) analysis.
Results: Promoter hyper methylation of the GSTP1 gene in voided urine samples was found in 71 of 78 (91%) males with PCa and in 7 of 57 (12%) males with BPH. The sensitivity and specificity of GSTP1 in discriminating between PCa and BPH males were 97% and 88%, respectively.
Conclusion: Gene analysis of GSTP1 using conventional MSP in urine specimens can be used as a non-invasive biomarker to distinguish between men with malignant and benign prostatic diseases.
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Muhammad Hashim Mengal
Department of Microbiology University of Balochistan, Quetta, Pakistan
Muhammad Kamran Taj
Centre for Advanced Studies in Vaccinology and Biotechnology, Quetta, Pakistan
Imran Nawaz
Department of Tumor and Cell Biology Karolinska Institute Stockholm Sweden