HISTO-MORPHOMETRIC ANALYSIS OF UTERINE TISSUE IN ALBINO MICE FOLLOWING EXPOSURE TO THE ANTHROPOGENIC CHEMICAL THIAMETHOXAM
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Keywords
Histology, Histomorphometry, Actara, ANOVA
Abstract
The use of Thiamethoxam (Brand name Actara 25 WG) is commonly used as insecticide mostly in tea gardens and paddy field as well to destroy the bugs and beetles and eliminates a broad range of soil pests. The present study was conducted to analyze the effect of thiamethoxam on uterine endometrial lining of Swiss albino mice. Six adult mice were divided into three groups of which Group-1 consists of two untreated mice maintained in laboratory condition, the Group-2 consists of two mice given daily Actara at the dose of 2mg/gm body weight and the Group-3 consists of two mice given daily Actara at the dose of 4mg/mg body weight for a period of 14 days administered via oral gavage. Histopathological study was performed following the processes of microtomy per the method of (Luna et al., 1968), and histomorphometric study was performed as per method of (Girija et al., 2015). Histopathological changes like endometrial edema, endometrial epithelial hyperplasia of the mucosa or glands, hyperplasia/edema of the myometrium and the result is significant at p < 0.005. when morphometric analysis of the epithelial layers of the uterus study were done by one-way ANOVA between the control and treated groups.
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Manzur Hassan
M.Sc Student, Department of Zoology, Bhattadev University, Pathsala, Assam-781325.
Dr. Akshay Kumar Haloi
Assistant Professor, Department of Zoology, Bhattadev University, Guwahati, Assam-7811325.
Dr. Puranjit Das
Assistant Professor, Department of Zoology, Bhattadev University, Guwahati, Assam-7811325.
Jinti Das
M.Sc Student, Department of Zoology, Gauhati University, Guwahati-781014.
Shamim MD H.A. Roshid
M.Sc Student, Department of Chemistry, Bodoland University, Kokrajhar-783370.