ICP-OES ANALYSIS OF ESSENTIAL, HEAVY, AND TRACE ELEMENTS IN THE MEDICINAL PLANTS OF UMERKOT DISTRICT, SINDH, PAKISTAN
Main Article Content
Keywords
Elements, Fagonia indica, Ipomoea carnea, Tinospora malabarica, Medicinal Plants, ICP-OES
Abstract
In this study, samples of medicinal plants from District Umerkot, Sindh, were analyzed for determining the concentrations of 17 essential elements, heavy metals, and trace metals such as Al, As, B, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Na, Ni, P, Pb, Se, and Zn. Determination of elemental profile of medicinal plants which are being used for therapeutic purposes in different regions. The analysis employed a 700 series Agilent inductively coupled plasma optical emission spectrometer (ICP-OES) coupled with ultrasonic CETAC U-6000AT + Nebulizer, following microwave-assisted acid digestion of the plant samples with MDS-6G closed microwave digester. The findings revealed significant concentrations of trace elements in medicinal plant extracts from the Umerkot, Sindh. Notably, Ipomoea Carnea samples exhibited elevated concentrations of essential elements, heavy metals, and trace metals such as 386.1, 0.0320, 0.8079,167.4, 0.194, 0.0016, 0.376, -1.932, 5.787, 33.72, 2.242, 15.56, 0.0115, 0.4936, 0.2371, 0.0494, 0.4785 mg/Kg, respectively. Fagonia indica samples displayed concentrations of 5.127, 0.0004, 0.6850, 121.7, 0.0036, 0.0024, 0.0181, 1.879, 1.844, 32.83, 0.3300, 11.83, 0.0019, 0.4054, 0.0054, 0.0202, and 0.0202 mg/Kg, respectively. However, Tinospora malabarica exhibited concentrations of 69.03, 0.0361, 1.555, 384.8, 0.0121, 0.0040, 0.0427, 0000, 7.100, 80.33, 3.242, 17.45, 0.0250, 0.14080, 9.550, 0.0181, 2.572 mg/Kg for Al, As, B, Ca, Cd, Co, Cr, Cu, Fe, Mg, Mn, Na, Ni, P, Pb, Se, and Zn, respectively. These elements can play a pivotal role in the plants' curative effects on human health.
References
[2] Tolo U, Kahwa C, Nuwagira I, Weisheit UA, Ikiriza H. Medicinal plants used in treatment of various diseases in the Rwenzori Region, Western Uganda. 2023; Ethnobot. Res. Appl., 25:1–16.
[3] Chhavi S, Sharma A, Dhiman A. An update on phytochemistry and therapeutic properties of Ipomoea carnea. J Pharmacogn Phytochem 2021; 10:01-06.
[4] Aziz MA, Adnan M, Khan AH, Shahat AA, Al-Said MS, Ullah R. Traditional uses of medicinal plants practiced by the indigenous communities at Mohmand Agency, FATA, Pakistan. J. Ethnobiol. Ethnomed 2018; 14(2): 1-16
[5] Ali K, Khan H. Fagonia indica; A Review on Chemical Constituents, Traditional Uses and Pharmacological Activities. Curr Pharm Des. 2021; 27(22):2648-2660.
[6] Sundar M, Lingakumar K. Investigating the efficacy of topical application of Ipomoea carnea herbal cream in preventing skin damage induced by UVB radiation in a rat model. Heliyon. 2023; 9(9).
[7] Fortune Business Insights (FBI) 2022 Fortune Business Insights (FBI) Herbal medicine market size & covid-19 impact analysis retrieved 30 october 2022. by application (pharmaceutical & nutraceutical, food & beverages and personal care & beauty products), by form (powder, liquid & gel, and tablets & capsule), and regional forecast. Market Res. Report. Food Suppl., Herbal Med. (2022), pp. 2022-2029. https://www.fortunebusinessinsights.com/herbal-medicine-market-106320.
[8] World Health Organization (WHO) 2003. World Health Organization (WHO).Guidelines On Good Agricultural and Collection Practices (GACP) For Medicinal Plants. WHO, Geneva, Switzerland (2003).
[9] Soetan KO, Olaiya CO, Oyewole OE. The importance of mineral elements for humans, domestic animals and plants: A review. Afr. J. Food Sci. 2010; 31;4(5):200-22.
[10] White PJ, Brown P. Plant nutrition for sustainable development and global health. Ann. Bot. 2010; 1;105(7):1073-80.
[11] Mehri A. Trace elements in human nutrition (II)–an update. Int. J. Prev. Med.. 2020 Jan 1;11(1):2.
[12] Mitra S, Chakraborty AJ, Tareq AM, Emran TB, Nainu F, Khusro A, Idris AM, Khandaker MU, Osman H, Alhumaydhi FA, Simal-Gandara J. Impact of heavy metals on the environment and human health: Novel therapeutic insights to counter the toxicity. J. King Saud Univ. Sci. 2022; 1;34(3):101865.
[13] Fagbohun OF, Olawoye B, Oriyomi OV, Joseph JS. Multivariate analyses of selected trace elements from Kigelia africana (Lam.) Benth. plant by ICP-OES: a chemometrics approach. JTEMIN. 2023; 1;5:100081.
[14] Barreca S, Orecchio S, Orecchio S, Abbate I, Pellerito C. Macro and micro elements in traditional meals of Mediterranean diet: Determination, estimated intake by population, risk assessment and chemometric analysis. J. Food Compos. Anal. 2023;1;123:105541.
[15] Jomova K, Makova M, Alomar SY, Alwasel SH, Nepovimova E, Kuca K, Rhodes CJ, Valko M. Essential metals in health and disease. Chem Biol Interact. 2022;1;367:110173.
[16] Briffa J, Sinagra E, Blundell R. Heavy metal pollution in the environment and their toxicological effects on humans. Heliyon. 2020; 1;6(9).
[17] Genchi G, Lauria G, Catalano A, Sinicropi MS, Carocci A. Biological activity of selenium and its impact on human health. Int. J. Mol. Sci.. 2023;30;24(3):2633.
[18] Liu L, Luo P, Wen P, Xu P. The role of magnesium in the pathogenesis of osteoporosis. Front. Endocrinol. 2024; 6;15:1406248.
[19] Austin DF. Ipomoea carnea jacq. vs. Ipomoea fistulosa Mart. ex Choisy. 1977; Taxon.26:235-238.
[20] Fatima N, Rahman MM, Khan MA, Fu J. A review on Ipomoea carnea: pharmacology, toxicology and phytochemistry. J Altern Complement Med. 2014;11:55-62.
[21] Wadnerwar N, Deogade M. Future perspectives of Therapeutic Claims of an Ethnopharmacological Drug Ipomoea carnea Jacq.-A Critical Review. International Journal of Ayurvedic Medicine. 2021;12:177-184.
[22] Haraguchi M, Gorniak SL, Ikeda K, Minami Y, Kato A, Watson AA, Nash RJ, Molyneux RJ, Asano N. Alkaloidal components in the poisonous plant, Ipomoea carnea (Convolvulaceae). J. Agric. Food Chem. 2003; 51:4995-5000.
[23] Lekshmipriya S, Pandya M, Prajapati B. Analytical Profile of an Unparalleled Drug Tinospora crispa WRT Guduchi. J. Pharm. Res. Int. 2021; 33:195-203.
[24] Mainasara MM. Documentation and evaluation of medicinal plants with potential for breast cancer therapy as used by Jakun Community of Kampung Peta, Mersing, Johor Universiti Tun Hussein Onn Malaysia. 2019.
[25] Perry LM, Metzger J. Medicinal plants of East and Southeast Asia: attributed properties and uses. 1980.
[26] Rizvi MA, Ali SA. Medicinal flowers of Pakistan. Int J Adv Res. 2016;4:1313-1341.
[27] Nidhi P, Swati P, Krishnamurthy R. Indian Tinospora species: natural immunomodulators and therapeutic agents. Int J Pharm Biol Chem Sci. 201; 2:01-09.
[28] Nutham N, Sakulmettatham S, Klongthalay S, Chutoam P, Somsak V. Protective effects of Tinospora crispa stem extract on renal damage and hemolysis during Plasmodium berghei infection in mice. Journal of pathogens. 2015; 738608.
[29] Ahmad VU, Basha A. Spectroscopic Data of Saponins: The Triterpenoid Glycosides, 3-Volume Set: CRC press. 2000.
[30] Soomro A, Jafarey N. 2003. Effect of Fagonia indica on experimentally produced tumours in rats. JPMA-Journal of Pakistan Medical Association.53:224-225.
[31] Hammiche V, Maiza K. Traditional medicine in Central Sahara: pharmacopoeia of Tassili N’ajjer. J. Ethnopharmacol. 2006; 105:358-367.
[32] Khattak KF. Microbiological quality assessment of commercially available medicinal plants in Peshawar city, Pakistan. Pak J Bot. 2012;44:1203-1208.
[33] Abirami V, Khosa R, Dhar S, Sahai M. Investigation on Fagonia cretica–its effect on hormonal profile and immunomodulation in rats. Anc. Sci. Life. 1996; 15:259-263.
[34] Mahmood A, Qureshi RA, Mahmood A, Sangi Y, Shaheen H, Ahmad I, Nawaz Z. Ethnobotanical survey of common medicinal plants used by people of district Mirpur, AJK, Pakistan. J Med Plants Res. 2011. 5:4493-4498.
[35] Nagaraj DS, Venkateswarlu B. Antipyretic effect of aqueous extract of Fagonia cretica L. whole plant on brewer's yeast induced pyrexia in wistar rats. Int J Pharmacol Toxicol. 2013; 3:39-42.
[36] Hussain A, Zia M, Mirza B. Cytotoxic and antitumor potential of Fagonia cretica L. Turk J Biol. 2007; 31:19-24.
[37] Seyal RA, Awan H, Tareen S. Can we really treat thalassemia major. Proceedings of the Pakistan Academy of Sciences. 2013; 50:315-325.
[38] Ab Manan WN, Zulkurnain N. Determination of selected heavy metal concentrations in water spinach (Ipomea aquatica). In Materials Science Forum 2021 Apr 29 (Vol. 1025, pp. 279-283).
[39] Amaechi M, Onwuka SU. Determination of the Presence and Level of Heavy Metals in Soils of Automobile Workshops in Awka, Anambra State. Int. J. Environ. Pollut. Res. 2021; 3;9(3):18-32.
[40] Rahal A, Prakash A, Verma AK, Kumar V, Roy D. Proximate and elemental analyses of Tinospora cordifolia stem. Pak J Biol Sci: PJBS. 2014; 1;17(5):744-7.
[41] Bakhsh W, Memon N, Maria G, Ullah I, Lanjwani AH, Memon AN. Metal composition of Fagonia indica of Kech, Balochistan, Pakistan. Rawal Med J. 2019; 22;44(2):398.
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Khadim Ali Gishkori
Pakistan Standard Quality Control Authority, Karachi-Pakistan
Saba Naz
Dr. M. A. Kazi Institute of Chemistry, University of Sindh,76080, Jamshoro-Pakistan
Aftab Ahmed Kandhro
Dr. M. A. Kazi Institute of Chemistry, University of Sindh,76080, Jamshoro-Pakistan
Aijaz Panhwar
PCSIR, Laboratories Complex, Off University Road, 75280, Karachi- Pakistan.
Mohammad Younis Talpur
Dr. M. A. Kazi Institute of Chemistry, University of Sindh,76080, Jamshoro-Pakistan
Muhammad Yousif Seelro
Dr. M. A. Kazi Institute of Chemistry, University of Sindh,76080, Jamshoro-Pakistan
Nusrat Jalbani
PCSIR, Laboratories Complex, Off University Road, 75280, Karachi- Pakistan.