ANTIBIOFILM ACTIVITY OF PROBIOTIC LACTIC ACID BACTERIA FROM COCKROACH (PERIPLANETA AMERICANA) GUT
Main Article Content
Keywords
Cockroach, Lactic acid bacteria, Probiotic, Antibiotic resistance, Biofilm
Abstract
This study has been focused on Antibiofilm activity of probiotic lactic acid bacteria from cockroach (Periplaneta americana) gut. A total of 20 samples were collected from the cockroach gut isolating bacteria using a 10-fold serial dilution on agar plates incubated anaerobically. Further characterization of the isolated strains included assessing their morphology, Gram staining, and performing various biochemical tests. Isolated strains were evaluated for antimicrobial activity against indicators strains i.e., E. coli, p. aeruginosa, s. aureus and klebsiella. Wider zones of inhibition were observed in the case of LAB isolates against E. coli, with a range extending from 34mm to 20mm. Among 08 LAB strains 03 were resistant to streptomycin and vancomycin. Probiotic characterization included NaCl, pH, and bile salt tolerance, temperature resistance, auto-aggregation, co-aggregation, and cell surface hydrophobicity. Probiotic strains Lactobacillus plantarum CE56.8 and Lacticaseibacillus rhamnosus 5974 showed highest growth rate at 8% NaCl concentration. These strains displayed significant growth under various conditions, indicating their potential as probiotics. Importantly, these strains exhibited a notable ability to inhibit biofilm formation by E. coli (88%) and P. aeruginosa (67%), offer promising effect in combatting biofilm-related infections. explored promising possibilities in the domain of beneficial bacteria, including lactic acid bacteria isolated from the gut of cockroach and lays the foundation for future investigations. However further research is needed to fully understand and develop LAB-based therapeutics for such infections.
References
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23. Miquel, S., Lagrafeuille, R., Souweine, B., and Forestier, C. (2016). Anti-biofilm activity as a health issue. Frontiers in Microbiology, 7, 592.
24. Karygianni, L., Ren, Z., Koo, H., and Thurnheer, T. (2020). Biofilm matrixome: extracellular components in structured microbial communities. Trends in Microbiology, 28(8): 668-681.
25. Westhoff, S., Kloosterman, A. M., van Hoesel, S. F., van Wezel, G. P., and Rozen, D.E. (2021). Competition sensing changes antibiotic production in streptomyces.MBio, 12(1): e02729-02720.
26.Tshibangu-Kabamba, E., and Yamaoka, Y. (2021). Helicobacter pylori infection and antibiotic resistance from biology to clinical implications. Nature Reviews Gastroenterology and Hepatology, 18(9): 613-629.
27. Siddiqui, R., Elmashak, Y., and Khan, N. A. (2023). Cockroaches: a potential source of novel bioactive molecule (s) for the benefit of human health. Applied Entomology and Zoology, 58(1): 1-11.
28. Zhang, J., Zhang, Y., Li, J., Liu, M., and Liu, Z. (2016). Midgut transcriptome of the cockroach Periplaneta americana and its microbiota: digestion, detoxification and oxidative stress response. PloS one, 11(5): e0155254.
29. Krausova, G., Hyrslova, I., and Hynstova, I. (2019). In vitro evaluation of adhesion capacity, hydrophobicity, and auto-aggregation of newly isolated potential probiotic strains. Fermentation, 5(4): 100.
30. Sannathimmappa, M. B., Nambiar, V., and Aravindakshan, R. (2021). Antibiotics at the crossroads–Do we have any therapeutic alternatives to control the emergence and spread of antimicrobial resistance? Journal of Education and Health Promotion, 10, 876
31. Dlamini, Z. C., Langa, R. L., Aiyegoro, O. A., and Okoh, A. I. (2019). Safety evaluation and colonisation abilities of four lactic acid bacteria as future probiotics. Probiotics and Antimicrobial Proteins, 11(2): 397-402.
32. Palencia, S. L., García, A., and Palencia, M. (2022). Multiple surface interaction mechanisms direct the anchoring, co-aggregation and formation of dual-species biofilm between Candida albicans and Helicobacter pylori. Journal of Advanced Research, 35, 169-185.
33. Shazadi, K., Ahmad, S. Z., Ahmad, S. S., and Arshad, N. (2021). In vivo prophylactic efficacy of Lactobacillus reuteri MT180537 against aerobic vaginitis. Microbial Pathogenesis, 160, 105197.
34. Campana, R., van Hemert, S., and Baffone, W. (2017). Strain-specific probiotic properties of lactic acid bacteria and their interference with human intestinal pathogens invasion. Gut pathogens, 9(1): 1-12.
35. Salas-Jara, M. J., Ilabaca, A., Vega, M., and García, A. (2016). Biofilm forming Lactobacillus: new challenges for the development of probiotics.Microorganisms, 4(3), 35.
36. Shokri, D., Khorasgani, M. R., Mohkam, M., Fatemi, S. M., Ghasemi, Y., and Taheri- Kafrani, A. (2018). The inhibition effect of lactobacilli against growth and biofilm formation of Pseudomonas aeruginosa. Probiotics and Antimicrobial Proteins, 10(1): 34-42.
37. Arena, M. P., Capozzi, V., Spano, G., and Fiocco, D. (2017). The potential of lactic acid bacteria to colonize biotic and abiotic surfaces and the investigation of their interactions and mechanisms. Applied Microbiology and Biotechnology, 101, 2641-2657.
38. Shazadi, K., and Arshad, N. (2022). Evaluation of inhibitory and probiotic properties of lactic acid bacteria isolated from vaginal microflora. Folia Microbiologica, 67(3): 427-445.
39. Rajoka, M. S. R., Mehwish, H. M., Siddiq, M., Haobin, Z., Zhu, J., Yan, L., Shao, D., Xu, X., and Shi, J. (2017). Identification, characterization, and probiotic potential of Lactobacillus rhamnosus isolated from human milk. LWT, 84, 271- 280.
40. Roe, A. L., Boyte, M.-E., Elkins, C. A., Goldman, V. S., Heimbach, J., Madden, E., Oketch-Rabah, H., Sanders, M. E., Sirois, J., and Smith, A. (2022). Considerations for determining safety of probiotics: A USP perspective. Regulatory Toxicology and Pharmacology, 9, 105266.
41. Additives, E.P.o., and Feed, P.o.S.u.i. A. (2012). Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance. EFSA Journal, 10(6): 2740.
42. Akpınar Kankaya, D., and Tuncer, Y. (2020). Antibiotic resistance in vancomycin‐ resistant lactic acid bacteria (VRLAB) isolated from foods of animal origin. Journal of Food Processing and Preservation, 44(6): 14468.
43. de Melo Pereira, G. V., de Oliveira Coelho, B., Júnior, A. I. M., Thomaz-Soccol, V., and Soccol, C. R. (2018). How to select a probiotic? A review and update of methods and criteria. Biotechnology Advances, 36(8): 2060-2076.
2. Dhasarathan, P., AlSalhi, M. S., Devanesan, S., Subbiah, J., Ranjitsingh, A., Binsalah, M., and Alfuraydi, A. A. (2021). Drug resistance in Candida albicans isolates and related changes in the structural domain of Mdr1 protein. Journal of Infection and Public Health, 14(12): 1848-1853.
3. Rosini, R., Nicchi, S., Pizza, M., and Rappuoli, R. (2020). Vaccines against antimicrobial resistance. Frontiers in immunology, 11, 1048.
4. Clutterbuck, A., Woods, E., Knottenbelt, D., Clegg, P., Cochrane, C., and Percival, S. (2007).
5. Biofilms and their relevance to veterinary medicine. Veterinary Microbiology, 121(1-2): 1-17.
6. Sánchez, B., Delgado, S., Blanco‐Míguez, A., Lourenço, A., Gueimonde, M., and Margolles, A. (2017). Probiotics, gut microbiota, and their influence on host health and disease. Molecular Nutrition and Food Research, 61(1): 1600240.
7. Akova, B., Kıvanç, S., and Kıvanç, M. (2021). Antibiofilm effect of probiotic lactic acid bacteria against Bacillus spp obtained from the ocular surface. Eur. Rev. Med. Pharmacol. Sci, 25, 7799-7805.
8. Medina‐Félix, D., Garibay‐Valdez, E., Vargas‐Albores, F., and Martínez‐Porchas, M. (2023). Fish disease and intestinal microbiota: A close and indivisible relationship. Reviews in Aquaculture, 15(2): 820-839.
8. Flandroy, L., Poutahidis, T., Berg, G., Clarke, G., Dao, M.-C., Decaestecker, E., Furman, E., Haahtela, T., Massart, S., and Plovier, H. (2018). The impact of human activities and lifestyles on the interlinked microbiota and health of humans and of ecosystems. Science of the Total Environment, 627, 1018-1038.
9. Kanauchi, O., Andoh, A., AbuBakar, S., and Yamamoto, N. (2018). Probiotics and paraprobiotics in viral infection: clinical application and effects on the innate and acquired immune systems. Current Pharmaceutical design, 24(6): 710- 717.
10. Yadav, S., and Jha, R. (2019). Strategies to modulate the intestinal microbiota and their effects on nutrient utilization, performance, and health of poultry. Journal of Animal Science and Biotechnology, 10(1): 1-11.
11. Reddy, R. S., Swapna, L., Ramesh, T., Singh, T. R., Vijayalaxmi, N., and Lavanya, R. (2011).
12. Bacteria in oral health-probiotics and prebiotics a review. Int J Biol Med Res, 2(4): 1226-1233.
13. Raksasiri, B. V. (2016). Enhancing the efficiency to utilise of inulin from plants as a prebiotics in goat kid diets School of Animal Production Technology Institute of Agricultural Technology,9, 876.
14. Kumar, M., Nagpal, R., Kumar, R., Hemalatha, R., Verma, V., Kumar, A., Chakraborty, C., Singh, B., Marotta, F., and Jain, S. (2022). Corrigendum to “Cholesterol-Lowering Probiotics as Potential Biotherapeutics for Metabolic Diseases”. Journal of Diabetes Research,6, 334.
15. Kumar, V., Chauhan, M., Pophaly, S., Tripathi, A., Kumar, V., Asgar, S., and Agarwal, A. (2022). Preparation of fermented oat drink using lactococcal isolates, 119, 208.
16. Pandey, N., Malik, R., Kaushik, J., and Singroha, G. (2013). Gassericin A: a circular bacteriocin produced by lactic acid bacteria Lactobacillus gasseri. World Journal of Microbiology and Biotechnology, 29, 1977-1987.
17. Bhogoju, S., and Nahashon, S. (2022). Recent advances in probiotic application in animal health and nutrition: a review. Agriculture, 12(2): 304.
18. Mahto, K. U., Priyadarshanee, M., Samantaray, D. P., and Das, S. (2022). Bacterial biofilm and extracellular polymeric substances in the treatment of environmental pollutants: Beyond the protective role in survivability. Journal of Cleaner Production,14, 134759.
19. Giordani, B., Parolin, C., and Vitali, B. (2021). Lactobacilli as anti-biofilm strategy in oral infectious diseases: a mini review. Frontiers in Medical Technology, 3, 769172.
20. Schulze, A., Mitterer, F., Pombo, J. P., and Schild, S. (2021). Biofilms by bacterial human pathogens: Clinical relevance-development, composition, and regulation-therapeutical strategies. Microbial Cell, 8(2): 28.
21. Khelissa, S. O., Abdallah, M., Jama, C., Faille, C., and Chihib, N.-E. (2017). Bacterial contamination and biofilm formation on abiotic surfaces and strategies to overcome their persistence. J Mater Environ Sci, 8(9): 3326-3346.
22. Bhowmik, A., Malhotra, A., Jana, S., and Chauhan, A. (2021). Biofilm Formation and Pathogenesis. Analytical Methodologies for Biofilm Research, 6, 3-37.
23. Miquel, S., Lagrafeuille, R., Souweine, B., and Forestier, C. (2016). Anti-biofilm activity as a health issue. Frontiers in Microbiology, 7, 592.
24. Karygianni, L., Ren, Z., Koo, H., and Thurnheer, T. (2020). Biofilm matrixome: extracellular components in structured microbial communities. Trends in Microbiology, 28(8): 668-681.
25. Westhoff, S., Kloosterman, A. M., van Hoesel, S. F., van Wezel, G. P., and Rozen, D.E. (2021). Competition sensing changes antibiotic production in streptomyces.MBio, 12(1): e02729-02720.
26.Tshibangu-Kabamba, E., and Yamaoka, Y. (2021). Helicobacter pylori infection and antibiotic resistance from biology to clinical implications. Nature Reviews Gastroenterology and Hepatology, 18(9): 613-629.
27. Siddiqui, R., Elmashak, Y., and Khan, N. A. (2023). Cockroaches: a potential source of novel bioactive molecule (s) for the benefit of human health. Applied Entomology and Zoology, 58(1): 1-11.
28. Zhang, J., Zhang, Y., Li, J., Liu, M., and Liu, Z. (2016). Midgut transcriptome of the cockroach Periplaneta americana and its microbiota: digestion, detoxification and oxidative stress response. PloS one, 11(5): e0155254.
29. Krausova, G., Hyrslova, I., and Hynstova, I. (2019). In vitro evaluation of adhesion capacity, hydrophobicity, and auto-aggregation of newly isolated potential probiotic strains. Fermentation, 5(4): 100.
30. Sannathimmappa, M. B., Nambiar, V., and Aravindakshan, R. (2021). Antibiotics at the crossroads–Do we have any therapeutic alternatives to control the emergence and spread of antimicrobial resistance? Journal of Education and Health Promotion, 10, 876
31. Dlamini, Z. C., Langa, R. L., Aiyegoro, O. A., and Okoh, A. I. (2019). Safety evaluation and colonisation abilities of four lactic acid bacteria as future probiotics. Probiotics and Antimicrobial Proteins, 11(2): 397-402.
32. Palencia, S. L., García, A., and Palencia, M. (2022). Multiple surface interaction mechanisms direct the anchoring, co-aggregation and formation of dual-species biofilm between Candida albicans and Helicobacter pylori. Journal of Advanced Research, 35, 169-185.
33. Shazadi, K., Ahmad, S. Z., Ahmad, S. S., and Arshad, N. (2021). In vivo prophylactic efficacy of Lactobacillus reuteri MT180537 against aerobic vaginitis. Microbial Pathogenesis, 160, 105197.
34. Campana, R., van Hemert, S., and Baffone, W. (2017). Strain-specific probiotic properties of lactic acid bacteria and their interference with human intestinal pathogens invasion. Gut pathogens, 9(1): 1-12.
35. Salas-Jara, M. J., Ilabaca, A., Vega, M., and García, A. (2016). Biofilm forming Lactobacillus: new challenges for the development of probiotics.Microorganisms, 4(3), 35.
36. Shokri, D., Khorasgani, M. R., Mohkam, M., Fatemi, S. M., Ghasemi, Y., and Taheri- Kafrani, A. (2018). The inhibition effect of lactobacilli against growth and biofilm formation of Pseudomonas aeruginosa. Probiotics and Antimicrobial Proteins, 10(1): 34-42.
37. Arena, M. P., Capozzi, V., Spano, G., and Fiocco, D. (2017). The potential of lactic acid bacteria to colonize biotic and abiotic surfaces and the investigation of their interactions and mechanisms. Applied Microbiology and Biotechnology, 101, 2641-2657.
38. Shazadi, K., and Arshad, N. (2022). Evaluation of inhibitory and probiotic properties of lactic acid bacteria isolated from vaginal microflora. Folia Microbiologica, 67(3): 427-445.
39. Rajoka, M. S. R., Mehwish, H. M., Siddiq, M., Haobin, Z., Zhu, J., Yan, L., Shao, D., Xu, X., and Shi, J. (2017). Identification, characterization, and probiotic potential of Lactobacillus rhamnosus isolated from human milk. LWT, 84, 271- 280.
40. Roe, A. L., Boyte, M.-E., Elkins, C. A., Goldman, V. S., Heimbach, J., Madden, E., Oketch-Rabah, H., Sanders, M. E., Sirois, J., and Smith, A. (2022). Considerations for determining safety of probiotics: A USP perspective. Regulatory Toxicology and Pharmacology, 9, 105266.
41. Additives, E.P.o., and Feed, P.o.S.u.i. A. (2012). Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance. EFSA Journal, 10(6): 2740.
42. Akpınar Kankaya, D., and Tuncer, Y. (2020). Antibiotic resistance in vancomycin‐ resistant lactic acid bacteria (VRLAB) isolated from foods of animal origin. Journal of Food Processing and Preservation, 44(6): 14468.
43. de Melo Pereira, G. V., de Oliveira Coelho, B., Júnior, A. I. M., Thomaz-Soccol, V., and Soccol, C. R. (2018). How to select a probiotic? A review and update of methods and criteria. Biotechnology Advances, 36(8): 2060-2076.
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Aug 25, 2024
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Sana Muneer, Sania Naheed, & Ansar Zubair. (2024). ANTIBIOFILM ACTIVITY OF PROBIOTIC LACTIC ACID BACTERIA FROM COCKROACH (PERIPLANETA AMERICANA) GUT. Journal of Population Therapeutics and Clinical Pharmacology, 31(8), 2960-2972. https://doi.org/10.53555/8nszfv07
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Author Biographies
Sana Muneer
Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan
Sania Naheed
Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan
Ansar Zubair
Department of Zoology, University of Education, Lahore, Pakistan
How to Cite
Sana Muneer, Sania Naheed, & Ansar Zubair. (2024). ANTIBIOFILM ACTIVITY OF PROBIOTIC LACTIC ACID BACTERIA FROM COCKROACH (PERIPLANETA AMERICANA) GUT. Journal of Population Therapeutics and Clinical Pharmacology, 31(8), 2960-2972. https://doi.org/10.53555/8nszfv07