EXPLORING THE RELATIONSHIP BETWEEN HEMATOLOGICAL AND SEROLOGICAL MARKERS IN DENGUE FEVER CASES
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Keywords
Dengue fever, hematological markers, serological markers, platelet count, NS1 antigen, diagnostic accuracy
Abstract
Background: Dengue fever, a mosquito-borne viral illness, poses significant health challenges globally, with its severity varying widely among patients. Hematological and serological markers are crucial in diagnosing and managing dengue, yet their interrelationships remain underexplored. This study investigates these relationships to enhance diagnostic accuracy and guide treatment strategies.
Objective: To explore the correlations between hematological markers (platelet count, hematocrit levels, and white blood cell count) and serological markers (dengue-specific antibodies and NS1 antigen) in dengue fever patients and assess their clinical significance.
Study Setting& Design: This cross-sectional study was conducted at Microbiology department of Peoples University of Medical and Health Sciences For Women (PUMHSW), Nawabshah
Methodology: A cross-sectional study was conducted with 135 dengue patients at a designated healthcare facility. Hematological parameters were analyzed using standard hematology analyzers, while serological markers were tested via enzyme-linked immunosorbent assays (ELISA) and rapid diagnostic tests (RDTs). Disease severity was categorized based on WHO criteria. Correlation analysis was performed to determine the relationships between hematological and serological markers. Statistical analyses were conducted using SPSS (version 28.0) and R (version 4.2.1), with a significance level set at p < 0.05.
Results: The mean platelet count was 84.7 × 10³/µL, with 88.9% of patients testing positive for dengue IgM and 81.5% for dengue IgG. NS1 antigen was detected in 66.7% of patients. Significant negative correlations were found between platelet count and both dengue IgM (r = -0.52, p < 0.01) and IgG (r = -0.45, p < 0.01). A positive correlation was observed between hematocrit and NS1 antigen levels (r = 0.48, p < 0.01).
Conclusion: The study demonstrates that hematological and serological markers are interrelated and provide valuable insights into disease severity and progression. These findings support the integration of both marker types in dengue diagnosis and management, potentially improving patient outcomes and guiding clinical decisions.
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Sandhiya Kumari
MBBS, M.Phil, Lecturer at INDUS Medical College Tando Mohmmad Khan
Jaya Kumari
MBBS, Medical Officers at INDUS Medical College Tando Mohmmad Khan
Shahzeen Fatima
MBBS, M.Phil in Microbiology from Peoples University of Medical and Health Sciences For Women (PUMHSW), Nawabshah
Amna Safdar
MBBS, M.Phil, Senior Demonstrator, Department of BiochemistryIslam Medical College, Sialkot
Rabail Bashir
MBBS, (M.Phil)Instructorat Peoples University ofMedical and Health Sciences for Women, Nawabshah
Arooj Ali
MBBS, CRCP (M.Phil) Lecturer at Department of Pathology People University of Medical and Health Science for Women, Nawabshah