EXPLORATION OF THE GOLD NANOPARTICLES–DNA INTERACTION: A NOVEL APPROACH TOWARDS RAPID CANCER DIAGNOSIS
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Keywords
DNA Interaction, Cancer Diagnosis, Nanotechnology
Abstract
Unique optical, physical, and biological characteristics of gold nanoparticles make them suitable for use in medicine, particularly in the detection of cancer. This study synthesized spherical monodispersed gold nanoparticles (AuNPs) by extending a little from established Turkevich, Frens via varying the concentration of HAuCl4/Citrate ratios. These freshly synthesized red color AuNPs are further characterized for size, size distribution, shape and monodispersity by UV/Vis spectrophotometer, DLS, TEM and AFM studies. Next genomic DNA was extracted from cancer and normal individuals’ blood samples by standard protocol and is further characterized by Nano Drop spectrophotometer. It was hypothesized that DNA has diverse tendencies to get adsorb onto AuNPs surface in colloidal solution form. Using this observation, we had planned this novel project based on color variations related to interaction of 40nm citrate capped AuNPs conjugated to non-functionalized genomic DNA both from cancer and normal samples. SPR peak stability vs SPR shift towards longer wavelength upon salt addition has been studied thoroughly, so this novel spectroscopic technique analyzed sample data set (both cancer and normal genomic DNA) adsorption and it was observed to show parabolic trend with SPR peak stability at 536nm in almost all Breast cancer samples where solution color remained red upon salt addition, so this led the basis of cancer detection, while healthy samples showed broadened SPR peak with diminished absorption values at SPR peak showing particle aggregation visible to eye as blue/grey color. Analysis results obtained from colorimetry and UV/Vis spectroscopy for monodisperse to polydisperse AuNPs colloidal solution expose that bigger AuNPs, that make the major volume of the solution, could overshadow the presence of smaller ones and maybe we need higher concentration of DNA to stabilize these AuNPs against aggregation upon salt addition.
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Tahira Ahmed
University of Agriculture Faisalabad, Pakistan
Yasir Jamil
University of Agriculture Faisalabad, Pakistan
Sajid Bashir
Pakistan Insititute of Nuclear Medicine (PINUM)
Yasir Javed
University of Agriculture Faisalabad, Pakistan
Amer Jamil
University of Agriculture Faisalabad, Pakistan