BOERHAVIA DIFFUSA: MEDICINAL POTENTIAL, ANTIOXIDANT AND ANTICANCER EFFECTS, WITH M-CSF AND GM-CSF GENE EXPRESSION ANALYSIS IN HCT116 CELL LINES.
Main Article Content
Keywords
Boerhavia diffusa, Flavonoids, Antioxidant activity, Anticancer potential, HCT116 cell lines
Abstract
Humans historically relied on nature for essential needs, including medicine. Medicinal plants, especially in developing nations, play a vital role in healthcare. Boerhavia diffusa, known as Punarnava, is an herb with diverse phytoconstituents, offering various health benefits. This study aimed to evaluate the phytoconstituent value, specifically total flavonoid content and antioxidant activity of Boerhavia diffusa. Additionally, the research explored its anticancer potential, focusing on the HCT116 cell line. Boerhavia diffusa leaves were extracted using hexane, chloroform, methanol, and water. Phytochemical analysis was conducted, and the total flavonoid content was estimated. Antioxidant activity was assessed using DPPH, FRAP, and H2O2 assays. Anticancer activity was examined on HCT116 cell lines, and potent extracts underwent column purification. The active fractions were further purified using HPLC. The isolated compound (Kaempferol) was tested for anticancer activity, and its IC50 was determined. Gene expression analysis was performed to evaluate the regulatory effects of Kaempferol on M-CSF and GM-CSF genes. Methanol and water extracts showed higher flavonoid content and antioxidant activity compared to hexane and chloroform extracts. Kaempferol, isolated from water extract, exhibited significant anticancer activity against HCT116 cell lines with an IC50 of 56.144 µg/ml. Gene expression analysis indicated Kaempferol's potential regulatory role in M-CSF and GM-CSF genes. Boerhavia diffusa extracts, particularly methanol and water extracts, demonstrated potent antioxidant and anticancer properties. Kaempferol, identified as a key bioactive compound, showed significant cytotoxicity against HCT116 cells and potential regulatory effects on immune-related genes. These findings support the medicinal potential of Boerhavia diffusa and warrant further exploration in healthcare applications.
References
[2] Sharma, A., del Carmen Flores-Vallejo, R., Cardoso-Taketa, A., & Villarreal, M. L. (2017). Antibacterial activities of medicinal plants used in Mexican traditional medicine. Journal of ethnopharmacology, 208, 264-329. https://doi.org/10.1016/j.jep.2016.04.045
[3] Benabderrahim, M. A., Yahia, Y., Bettaieb, I., Elfalleh, W., & Nagaz, K. (2019). Antioxidant activity and phenolic profile of a collection of medicinal plants from Tunisian arid and Saharan regions. Industrial Crops and Products, 138, 111427. https://doi.org/10.1016/j.indcrop.2019.05.076
[4] Gunathilake, K. D. P. P., Ranaweera, K. K. D. S., & Rupasinghe, H. P. V. (2018). Change of phenolics, carotenoids, and antioxidant capacity following simulated gastrointestinal digestion and dialysis of selected edible green leaves. Food chemistry, 245, 371-379.
[5] Hazafa, A., Rehman, K. U., Jahan, N., & Jabeen, Z. (2020). The role of polyphenol (flavonoids) compounds in the treatment of cancer cells. Nutrition and cancer, 72(3), 386-397. https://doi.org/10.1080/01635581.2019.1637006
[6] Buyel, J. F. (2018). Plants as sources of natural and recombinant anti-cancer agents. Biotechnology advances, 36(2), 506-520. https://doi.org/10.1016/j.biotechadv.2018.02.002
[7] Nayak, P., & Thirunavoukkarasu, M. (2016). A review of the plant Boerhaavia diffusa: its chemistry, pharmacology and therapeutical potential. J. Phytopharmacol, 5(2), 83-92.
[8] Struwig, M., & Siebert, S. J. (2013). A taxonomic revision of Boerhavia (Nyctaginaceae) in southern Africa. South African Journal of Botany, 86, 116-134. https://doi.org/10.1016/j.sajb.2013.02.172
[9] Ali, S., Singh, A., & Anjali, A. S. K. (2022). Study On Boerhavia Diffusa Linn. As An Important Medicinal Plant Resource And Its Market Economy: A Review. Journal of Pharmaceutical Negative Results, 13(8) 474-478. https://doi.org/10.47750/pnr.2022.13.S08.61
[10] Prathapan, A., Varghese, M. V., Abhilash, S., Mathew, A. K., Nair, A., Nair, R. H., & Raghu, K. G. (2017). Polyphenol rich ethanolic extract from Boerhavia diffusa L. mitigates angiotensin II induced cardiac hypertrophy and fibrosis in rats. Biomedicine & Pharmacotherapy, 87, 427-436. https://doi.org/10.1016/j.biopha.2016.12.114
[11] Chauhan, o., & Sharma, s. (2021). Gene expression analysis of therapeutically active antiproliferative polyphenolic fractions extracted from Rubus ellipticus on human cervical cancer cells (hela). uttar pradesh journal of zoology, 178-194.
[12] Mir, M. A., Parihar, K., Tabasum, U., & Kumari, E. (2016). Estimation of alkaloid, saponin and flavonoid, content in various extracts of Crocus sativa. Journal of Medicinal Plants Studies, 4(5), 171-174.
[13] Mahesh, A. R., Ranganath, M. K., & Harish Kumar, D. R. (2013). Enrichment of flavonoids from the methanolic extract of Boerhaavia diffusa roots by partitioning technique. Research Journal of Chemical Sciences, 3(1), 43-47.
[14] Kewlani, P., Tiwari, D., Rawat, S., & Bhatt, I. D. (2023). Pharmacological and phytochemical potential of Rubus ellipticus: a wild edible with multiple health benefits. Journal of Pharmacy and Pharmacology, 75(2), 143-161. https://doi.org/10.1093/jpp/rgac053
[15] Shibu Prasanth, S. C. R., & Chandran, P. (2017). Phytochemical and antimicrobial analysis of leaf samples of different Rubus species. Intl J Chem Tech Res, 10(4), 359-368.
[16] Pontis JA, LA Mendonca, AD Costa, SJRD Silva, A Flach (2014) Color, phenolics and flavonoid content and antioxidant activity of honey from Roraima, Brazil. Food Sci Tech 34: 69-73.
[17] Sharma, U. S., & Kumar, A. (2011). In vitro antioxidant activity of Rubus ellipticus fruits. Journal of advanced pharmaceutical technology & research, 2(1), 47. https://doi.org/10.4103%2F2231-4040.79805
[18] Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Analytical biochemistry, 239(1), 70–76. https://doi.org/10.1006/abio.1996.0292
[19] Kumar, T., Pande, K. K., Mathur, A., Koranga, M., Pande, B., & Sharma, H. (2020). Antimicrobial And Antioxidant Profile of Solvent Extracts of Fruits of Rubus Ellipticus. Journal of Advanced Scientific Research, 11(04), 100-105.
[20] Zhang, H., Wang, W., Fu, Z. M., Han, C. C., & Song, Y. (2014). Study on comparison of extracting fucoxanthin from Undaria pinnatifida with percolation extraction and refluxing methods. Zhongguo Shipin Tianjiaji, 9, 91-95.
[21] Mbarek, A. L., Ait Mouse, H., Elabbadi, N., Bensalah, M., Gamouh, A., Aboufatima, R., ... & Zyad, A. (2007). Anti-tumor properties of blackseed (Nigella sativa L.) extracts. Brazilian Journal of Medical and Biological Research, 40, 839-847. https://doi.org/10.1590/S0100-879X2006005000108
[22] Rio, D. C., Ares, M., Jr, Hannon, G. J., & Nilsen, T. W. (2010). Purification of RNA using TRIzol (TRI reagent). Cold Spring Harbor protocols, 2010(6), pdb.prot5439. https://doi.org/10.1101/pdb.prot5439
[23] Lu, L., Wang, Y., Ou, R., Feng, Q., Ji, L., Zheng, H., Guo, Y., Qi, X., Kong, A. N., & Liu, Z. (2018). DACT2 Epigenetic Stimulator Exerts Dual Efficacy for Colorectal Cancer Prevention and Treatment. Pharmacological research, 129, 318–328. https://doi.org/10.1016/j.phrs.2017.11.032
[24] Bandyopadhyay, S., Romero, J. R., & Chattopadhyay, N. (2008). Kaempferol and quercetin stimulate granulocyte-macrophage colony-stimulating factor secretion in human prostate cancer cells. Molecular and cellular endocrinology, 287(1-2), 57–64. https://doi.org/10.1016/j.mce.2008.01.015
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Rohit Kumar
Department of Biotechnology, OPJS University, Churu Rajasthan, 331303
Kamlesh Kumar
Department of Biotechnology, OPJS University, Churu Rajasthan, 331303