ISOLATION AND IDENTIFICATION OF POTENTIAL ANTI-CANCER PIGMENT-PRODUCING BACTERIA FROM THE GUT OF FISH DEVARIO AEQUIPINNATUS
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Keywords
Anti-Cancerous Property, Devario Aequipinnatus, Pigment Producing Bacteria, GC- MS Analysis
Abstract
Cancer is a major public health problem in the world and it is the second leading cause of mortality worldwide. One in 4 deaths in the world is due to cancer. According to WHO Cancer is a generic term for a large group of diseases that can affect any part of the body. Currently, there are several conventional anti-cancer treatments, including surgery, chemotherapy, and radiotherapy. But these treatments are costly and have harmful side effects. Therefore, the exploration of newer and better anti-cancer agents with low side effects is essential to rectify this deleterious situation.
In the present study pigment producing bacteria from the gut of Devario aequipinnatus was isolated and identified. The pigment was characterized for possible anti-cancer property. The purification of pink pigment produced by the bacterial isolate was carried out using solvent extraction method post solvent optimization. Characterization of the pigment was done by GC- MS analysis and cytotoxicity profiling by MTT assay and anti-cancer property was evaluated via Acridine orange- ethidium bromide (AO/EB) staining. The overall completed study revealed that the pink pigment from Massilia haematophila CCUG 38318(T), isolated from Devario aequipinnatus fish gut has potential anti-cancerous property.
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Sreeshma P.V
Department of Biology, St. Mary’s College, Thrissur, Kerala, India -680020
Jumana Karadan
Department of Biology, St. Mary’s College, Thrissur, Kerala, India -680020
Anu P. Abhimannue
Assistant professor, Department of Biotechnology, St. Mary’s College, Thrissur, Kerala, India -680020