FREQUENCY OF DENTURE HYGIENE PRACTICES AND THEIR IMPACT ON ORAL MICROBIOME IN PATIENTS WEARING REMOVABLE PARTIAL DENTURES
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Keywords
Denture hygiene, Oral microbiome, Removable partial dentures, Oral health, Denture care practices, Microbial load
Abstract
Background: Denture hygiene practices are critical in maintaining oral health, particularly in patients wearing removable partial dentures..
Objectives: To determine the frequency of denture hygiene practices and their impact on oral microbiome in patients wearing removable partial dentures.
Methods: The study was conducted using a cross-sectional design, enrolling 150 patients who had been using RPDs for at least six months at Dental Department Shahida Islam Medical &Dental College, Lodhran from Jan 2021 to June 2021. Inclusion criteria for the study included adult patients aged 18-75 years who were wearing RPDs and were willing to participate in the study. All participants provided written informed consent prior to inclusion in the study. The questionnaire was designed to assess participants' demographic information, denture hygiene practices (including frequency and methods of cleaning), and any reported oral health issues. The data were analyzed using SPSS software (version 26.0).
Results: The majority of participants (40%) were between 51-65 years old, with a higher proportion of males (56.7%) compared to females (43.3%). Patients who cleaned their dentures less frequently (less than once daily) had significantly higher mean counts of Streptococcus mutans, Candida albicans, and Lactobacillus species compared to those who cleaned more often (p<0.05). The microbial load decreased with more frequent cleaning, particularly for those who cleaned their dentures after every meal. The combination of mechanical and chemical cleaning methods was associated with the lowest prevalence of oral lesions (11.1%), whereas mechanical brushing alone resulted in a higher incidence of oral lesions (25.0%).
Conclusion: In conclusion our study confirms that the frequency of denture cleaning has a significant impact on the prevalence of oral microorganisms. Less frequent cleaning is associated with higher counts of Streptococcus mutans, Candida albicans, and Lactobacillus species, highlighting the importance of regular denture maintenance.
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Fareed Ahmad
Associate Professor, Department of Oral Medicine Institute of Dentistry, CMH Lahore Medical College, Lahore, Pakistan, National University of Medical Sciences, Rawalpindi, Pakistan
Ali Farooq
Associate professor, Oral & Maxillofacial Surgery department Sharif Medical and Dental College Lahore
Usman Qadir Khan
Associate Professor, Oral & Maxillofacial surgery Department Shahida Islam Medical &Dental College, Lodhran
Munawar Hussain
M. Phil Oral biology, Senior demonstrator, Oral Biology Shahida Islam medical and dental college Lodhran
Muhammad Aamir Rafique
Assistant Professor, Proathodontics, Demontmorency College Of Dentistry, Lahore
Sobia Siddique
Associate Professor Oral Pathology, HBS Dental College, Islamabad
Saira khalid
BDS, M.Phil, PHD Scholar, Associate Professor Science Of Dental Materials University Of Health Sciences, Lahore