IN VITRO ANTIMICROBIAL ACTIVITY OF POLYHERBAL OINTMENT AGAINST HUMAN PATHOGENIC BACTERIA

Main Article Content

Mahajan Sachin.M.
Bhandari Harshal. R.

Keywords

Medicinal plants, Phytochemical Investigation, Polyherbal Ointment, antimicrobial activity

Abstract

The aim of the study was assess the antimicrobial effect of some medicinal plants and its formulation against Escherichia coli, Candida albicans, Aspergillus niger. Medicinal plants are the important source of potentially useful structures for the development of novel chemotherapeutic agents. Historically, plants have provided a source of the development for novel drugs and plant derived drugs which have made large contributions to human health and wellbeing. The hydro-alcoholic extract of selected medicinal plant show presence of major phyto-constituent such as alkaloids, glycosides, steroids, flavonoids, tannins etc. The antimicrobial activities of selected plants extracts were evaluated using the disk diffusion method .In the present study, polyherbal formulation was screened against microorganism at the concentration of 100 /ml with reference standard DERMIKEM OC Cream. At above concentration there is antibacterial activity found in PHF A and B They not show antifungal activity.

Abstract 44 | PDF Downloads 12

References

1. Muqarrabun L, Ahmat N, Ruzaina SAS , Ismail NH, Sahidin I. Medicinal uses, phytochemistry and pharmacology of Pongamia pinnata (L.) Pierre: A review. J of Ethno, 150(2);2013:395-420.
2. Muqarrabun L, Ahmat N, Ruzaina SAS , Ismail NH, Sahidin I. Medicinal uses, phytochemistry and pharmacology of Pongamia pinnata (L.) Pierre: A review. J of Ethno, 150(2); 2013:395-420.
3. Khan M., Srivastava SK, Jain, ShyamSundan KV. Chemical compostion of fruit and stem essential oil of Lanatacamara form northen india. flavor frangnance Journal 18(5),2003;376-379.
4. Begum S, Zehra SQ, Wahb A, Siddiqui BS. Triterpenoidal secondary matabolites from lanara camaralinn. Chem. info, 38(4) 89; 2007; 1932-1941.
5. Vishwanathan AS, Basaavraj R. A Review on Vitexnegundo L.– A Medicinally Important Plant . EJBS 3 (1); 2010:30-42.
6. Banerji, A, Chadha MS, Malshet VG. Isolation of 5-hydroxy-3,6,7,3’,4’-pentamethoxyflavone from Vitexnegundo', Phytochemistry. 8; 1969:511-512.
7. Dholvitayakhun A, Trachoo N. Using scanning and transmission electron microscopy to investigate the antibacterial mechanism of action Annona squmosalinn. Journal Of Herbal Medicine; 7; 2017:31-36.
8. Gaurav P, Neelam B. Calotropis procera: A phytochemical and pharmacological review, TJPS 2016; 40 (3): 115-131.
9. Brüschweiler F, Stöcklin W, Stöckel K, Reichstein T. Glycosides of Calotropis procera R Br. Glycosides and glycon . Helv. Chim. Acta. 1969;52(7):2086-106.
10. Koushik M, Arkendu C, Somenath B, Raj B, Sonia A, Injamul H. A Review on various Biological and Pharmacological properties of Thevetia peruviana. Int. J. Adv.Res. Biol. Sci. 3(9);2016: 178-182.
11. Riverapasrana DM, Yahima EM, Gonzalez-aguiler GA. Phenolic and carotenoid profiles of papaya fruit and their content under low temperature sorage. J Sci Food Agric 2010;90(14):2358-65.
12. Sleigler DS, Poul GF, Nahsted A, leen R. Cyanogenitic glycoside and glucosides from Passiflora edulis and Carica papaya. Phytochemistry. 2002;60(8):873-82.
13. Tan QG, Cai XH, Dua ZZ, Luo XD.Three terpenoids and a tocopherol-related compound from Ricinus communis. Helv Chim Acta. 92 (12): 2762–8.
14. .Mohammad A, Alzohairy. Therapeutics Role of Azadirachta indica (Neem) and Their Active Constituents in Diseases Prevention and Treatment. Evid Based Complement Alternat Med. 2016; 7382506.
15. Rahmani AH, Almatroudi A, Alrumaihi F, Khan AA. Pharmacological and therapeutic potential of neem (Azadirachta indica). PhcogRev 2018;12:250-5.
16. Kokate C.K, Purohit A.P., Gokhale S.B, Textbook of pharmacognosy, Niraliprakashan.
17. Khandelwal K.R.,Textbook of practical pharmacognosy,Niraliprakashan.(page no 158, 159 ).
18. British Pharmacopoeia (BP) 1988.. Department of health and social security Scottish home and health department. Office of the British Pharmacopoeia Commission, UK, vol. 2, 713, 1988.
19. Mounyr B, Moulay S, Saad KI. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal. 2016; 6(2): 71–79.
20. Indian Pharmacopoeia 1996.